{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T20:35:39Z","timestamp":1778704539109,"version":"3.51.4"},"reference-count":42,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2021,1,22]],"date-time":"2021-01-22T00:00:00Z","timestamp":1611273600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"The Russian Science Foundation grant","award":["19-44-02027 (MSP)"],"award-info":[{"award-number":["19-44-02027 (MSP)"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>The processed pseudogene <jats:italic>PTENP1<\/jats:italic> is involved in the regulation of the expression of the <jats:italic>PTEN<\/jats:italic> and acts as a tumor suppressor in many types of malignances. In our previous study we showed that <jats:italic>PTENP1<\/jats:italic> methylation is present not only in tumor, but also in normal endometrium tissues of women over 45 years old. Here we used methylation-specific PCR to analyze methylation status of CpG island located near promoter region of <jats:italic>PTENP1<\/jats:italic> in malignant and non-malignant endometrium tissues collected from 236 women of different age groups. To confirm our results, we also analyzed RNA sequencing and microarray data from 431 women with endometrial cancer from TCGA database. We demonstrated that methylation of <jats:italic>PTENP1<\/jats:italic> is significantly increased in older patients. We also found an age-dependent increase in the level of <jats:italic>PTENP1<\/jats:italic> expression in endometrial tissue. According to our data, <jats:italic>PTENP1<\/jats:italic> methylation elevates the level of the pseudogene sense transcript. In turn, a high level of this transcript correlates with a more favorable prognosis in endometrial cancer. The data obtained suggested that <jats:italic>PTENP1<\/jats:italic> methylation is associated with age-related changes in normal and hyperplastic endometrial tissues. We assumed that age-related increase in <jats:italic>PTENP1<\/jats:italic> methylation and subsequent elevation of its expression may serve as a protective mechanism aimed to prevent malignant transformation of endometrial tissue in women during the perimenopause, menopause, and postmenopause periods.<\/jats:p>","DOI":"10.1371\/journal.pone.0243093","type":"journal-article","created":{"date-parts":[[2021,1,22]],"date-time":"2021-01-22T22:49:24Z","timestamp":1611355764000},"page":"e0243093","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":15,"title":["Methylation of the PTENP1 pseudogene as potential epigenetic marker of age-related changes in human endometrium"],"prefix":"10.1371","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6091-892X","authenticated-orcid":true,"given":"Tatyana 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