{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T22:10:12Z","timestamp":1781647812861,"version":"3.54.5"},"reference-count":68,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2020,12,8]],"date-time":"2020-12-08T00:00:00Z","timestamp":1607385600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p><jats:italic>Pseudomonas aeruginosa<\/jats:italic> is an opportunistic pathogen that can cause a variety of diseases especially in the hospital environment. However, this pathogen also exhibits antimicrobial activity against Gram-positive bacteria and fungi. This study aimed to characterize different virulence factors, secreted metabolites and to study their role in the suppression of <jats:italic>Candida<\/jats:italic> growth. Fifteen <jats:italic>P<\/jats:italic>. <jats:italic>aeruginosa<\/jats:italic> isolates were tested for their anticandidal activity against 3 different <jats:italic>Candida<\/jats:italic> spp. by the cross-streak method. The effect on hyphae production was tested microscopically using light and scanning electron microscopy (SEM). Polymerase chain reaction was used in the detection of some virulence genes. Lipopolysaccharide profile was performed using SDS-polyacrylamide gel stained with silver. Fatty acids were analyzed by GC-MS as methyl ester derivatives. It was found that 5 <jats:italic>P<\/jats:italic>. <jats:italic>aeruginosa<\/jats:italic> isolates inhibited all tested <jats:italic>Candida<\/jats:italic> spp. (50\u2013100% inhibition), one isolate inhibited <jats:italic>C<\/jats:italic>. <jats:italic>glabrata<\/jats:italic> only and 3 isolates showed no activity against the tested <jats:italic>Candida<\/jats:italic> spp. The <jats:italic>P<\/jats:italic>. <jats:italic>aeruginosa<\/jats:italic> isolates inhibiting all <jats:italic>Candida<\/jats:italic> spp. were positive for all virulence genes. GC-Ms analysis revealed that isolates with high anticandidal activity showed spectra for several compounds, each known for their antifungal activity in comparison to those with low or no anticandidal activity. Hence, clinical isolates of <jats:italic>P<\/jats:italic>. <jats:italic>aeruginosa<\/jats:italic> showed <jats:italic>Candida<\/jats:italic> species-specific interactions by different means, giving rise to the importance of studying microbial interaction in polymicrobial infections and their contribution to causing disease.<\/jats:p>","DOI":"10.1371\/journal.pone.0243418","type":"journal-article","created":{"date-parts":[[2020,12,8]],"date-time":"2020-12-08T20:54:38Z","timestamp":1607460878000},"page":"e0243418","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":12,"title":["Virulence profiles of some Pseudomonas aeruginosa clinical isolates and their association with the suppression of Candida growth in polymicrobial infections"],"prefix":"10.1371","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6094-5653","authenticated-orcid":true,"given":"Rehab Mahmoud","family":"Abd El-Baky","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sahar A.","family":"Mandour","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eman Farouk","family":"Ahmed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4216-8584","authenticated-orcid":true,"given":"Zeinab Shawky","family":"Hashem","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tim","family":"Sandle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Doaa Safwat","family":"Mohamed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2020,12,8]]},"reference":[{"key":"pone.0243418.ref001","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.micpath.2016.07.013","article-title":"Molecular detection of virulence genes in Pseudomonas aeruginosa isolated from children with Cystic Fibrosis and burn wounds in Iran","volume":"99","author":"F Faraji","year":"2016","journal-title":"Microbial pathogenesis"},{"issue":"1","key":"pone.0243418.ref002","first-page":"37","article-title":"A study of purified pyorubrin produced by local Pseudomonas aeruginosa","volume":"46","author":"S Kandela","year":"1997","journal-title":"Acta Microbiologica Polonica"},{"issue":"2","key":"pone.0243418.ref003","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1128\/JCM.32.2.525-527.1994","article-title":"Suppression of fungal growth exhibited by Pseudomonas aeruginosa","volume":"32","author":"J. Kerr","year":"1994","journal-title":"Journal of clinical microbiology"},{"issue":"3","key":"pone.0243418.ref004","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1046\/j.1469-8137.2003.00686.x","article-title":"Phenazines and their role in biocontrol by Pseudomonas bacteria","volume":"157","author":"TFC Chin-A-Woeng","year":"2003","journal-title":"New Phytologist"},{"issue":"1","key":"pone.0243418.ref005","doi-asserted-by":"crossref","first-page":"69","DOI":"10.3109\/07388559009038205","article-title":"Microbial fungicides: recent approaches to elucidating mechanisms","volume":"10","author":"N. Gutterson","year":"1990","journal-title":"Critical reviews in biotechnology"},{"issue":"1","key":"pone.0243418.ref006","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1111\/j.1439-0507.2006.01322.x","article-title":"Anticandidal activity of Pseudomonas aeruginosa strains isolated from clinical specimens","volume":"50","author":"I Kaleli","year":"2007","journal-title":"Mycoses"},{"issue":"6","key":"pone.0243418.ref007","doi-asserted-by":"crossref","first-page":"1379","DOI":"10.1111\/j.1365-2958.2010.07414.x","article-title":"Antifungal mechanisms by which a novel Pseudomonas aeruginosa phenazine toxin kills Candida albicans in biofilms","volume":"78","author":"DK Morales","year":"2010","journal-title":"Molecular microbiology"},{"issue":"3","key":"pone.0243418.ref008","first-page":"73","article-title":"Epidemiology of candidemia","volume":"17","author":"P. Sandven","year":"2000","journal-title":"Revista Iberoamericana de Micologia"},{"key":"pone.0243418.ref009","author":"FF Rinzan","year":"2009","journal-title":"Pseudomonas Aeruginosa-Candida Albicans Interactions From Ecological and Molecular Perspectives"},{"issue":"13","key":"pone.0243418.ref010","doi-asserted-by":"crossref","first-page":"1368","DOI":"10.7150\/ijms.18896","article-title":"Isolation and determination of four potential antimicrobial components from Pseudomonas aeruginosa extracts","volume":"14","author":"L-Q Xu","year":"2017","journal-title":"International journal of medical sciences"},{"issue":"7","key":"pone.0243418.ref011","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/S0966-842X(01)02094-7","article-title":"Virulence factors of Candida albicans","volume":"9","author":"RA Calderone","year":"2001","journal-title":"Trends in microbiology"},{"issue":"5","key":"pone.0243418.ref012","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1136\/jcp.52.5.385","article-title":"Pseudomonas aeruginosa pyocyanin and 1-hydroxyphenazine inhibit fungal growth","volume":"52","author":"J Kerr","year":"1999","journal-title":"Journal of clinical pathology"},{"issue":"1","key":"pone.0243418.ref013","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1111\/j.1574-6968.2008.01301.x","article-title":"Cell wall glycans and soluble factors determine the interactions between the hyphae of Candida albicans and Pseudomonas aeruginosa","volume":"287","author":"A Brand","year":"2008","journal-title":"FEMS microbiology letters"},{"key":"pone.0243418.ref014","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1146\/annurev.phyto.44.013106.145710","article-title":"Phenazine compounds in fluorescent Pseudomonas spp. biosynthesis and regulation","volume":"44","author":"DV Mavrodi","year":"2006","journal-title":"Annu Rev Phytopathol"},{"issue":"4","key":"pone.0243418.ref015","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1007\/s00284-006-0444-4","article-title":"Characterization of a phenazine-producing strain Pseudomonas chlororaphis GP72 with broad-spectrum antifungal activity from green pepper rhizosphere","volume":"54","author":"H Liu","year":"2007","journal-title":"Current microbiology"},{"issue":"3","key":"pone.0243418.ref016","doi-asserted-by":"crossref","first-page":"1663","DOI":"10.1021\/cr020473j","article-title":"Phenazine natural products: biosynthesis, synthetic analogues, and biological activity","volume":"104","author":"JB Laursen","year":"2004","journal-title":"Chemical reviews"},{"issue":"2","key":"pone.0243418.ref017","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1128\/AEM.01037-08","article-title":"Pseudomonas aeruginosa-Candida albicans interactions: localization and fungal toxicity of a phenazine derivative","volume":"75","author":"J Gibson","year":"2009","journal-title":"Applied and Environmental Microbiology"},{"issue":"1","key":"pone.0243418.ref018","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1099\/00222615-5-1-67","article-title":"The effect of 1-hydroxyphenazine and pyocyanin from Pseudomonas aeruginosa on mammalian cell respiration","volume":"5","author":"DE Stewart-Tull","year":"1972","journal-title":"Journal of medical microbiology"},{"issue":"1","key":"pone.0243418.ref019","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1172\/JCI112787","article-title":"Pyocyanin and 1-hydroxyphenazine produced by Pseudomonas aeruginosa inhibit the beating of human respiratory cilia in vitro","volume":"79","author":"R Wilson","year":"1987","journal-title":"The Journal of clinical investigation"},{"issue":"1","key":"pone.0243418.ref020","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1007\/s10156-012-0457-9","article-title":"Reduction of virulence factor pyocyanin production in multidrug-resistant Pseudomonas aeruginosa","volume":"19","author":"K Fuse","year":"2013","journal-title":"Journal of Infection and Chemotherapy"},{"issue":"7","key":"pone.0243418.ref021","doi-asserted-by":"crossref","first-page":"4275","DOI":"10.1128\/IAI.72.7.4275-4278.2004","article-title":"Pseudomonas aeruginosa pyocyanin is critical for lung infection in mice","volume":"72","author":"GW Lau","year":"2004","journal-title":"Infection and immunity"},{"issue":"2","key":"pone.0243418.ref022","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1128\/AM.24.2.219-225.1972","article-title":"Epidemiology of Pseudomonas aeruginosa in a burns hospital: surveillance by a combined typing system","volume":"24","author":"P Edmonds","year":"1972","journal-title":"Applied microbiology"},{"issue":"4","key":"pone.0243418.ref023","first-page":"556","article-title":"Degradation of castor oil and lipase production by Pseudomonas aeruginosa","volume":"5","author":"AA Amara","year":"2009","journal-title":"American-Eurasian J Agric Environ Sci"},{"issue":"3","key":"pone.0243418.ref024","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1128\/MR.43.3.422-442.1979","article-title":"Secondary metabolites of the fluorescent pseudomonads","volume":"43","author":"T Leisinger","year":"1979","journal-title":"Microbiological Reviews"},{"issue":"1","key":"pone.0243418.ref025","first-page":"7","article-title":"Pyocyanine biosynthetic genes in clinical and environmental isolates of Pseudomonas aeruginosa and detection of pyocyanine\u2019s antimicrobial effects with or without colloidal silver nanoparticles","volume":"14","author":"J Nowroozi","year":"2012","journal-title":"Cell Journal (Yakhteh)"},{"issue":"9","key":"pone.0243418.ref026","doi-asserted-by":"crossref","first-page":"4839","DOI":"10.1128\/IAI.68.9.4839-4849.2000","article-title":"Bacterial quorum sensing in pathogenic relationships","volume":"68","author":"TR De Kievit","year":"2000","journal-title":"Infection and immunity"},{"issue":"1","key":"pone.0243418.ref027","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1186\/1471-2180-12-281","article-title":"Evaluation of adhesion forces of Staphylococcus aureus along the length of Candida albicanshyphae","volume":"12","author":"ES Ovchinnikova","year":"2012","journal-title":"BMC Microbiology"},{"issue":"5","key":"pone.0243418.ref028","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1136\/jcp.52.5.385","article-title":"Pseudomonas aeruginosa pyocyanin and 1-hydroxyphenazine inhibit fungal growth","volume":"52","author":"JR Kerr","year":"1999","journal-title":"Journal of clinical pathology"},{"key":"pone.0243418.ref029","volume-title":"Proceedings of the international conference on cement microscopy","author":"PE Stutzman","year":"1999"},{"key":"pone.0243418.ref030","article-title":"Preparation of genomic DNA from bacteria","author":"K. Wilson","year":"2001","journal-title":"Current protocols in molecular biology"},{"key":"pone.0243418.ref031","first-page":"83","article-title":"Bacterial lipopolysaccharides extraction with phenol-water and further applications of the procedure","volume":"5","author":"O. Westphal","year":"1965","journal-title":"Methods Carbohydr Chem"},{"issue":"63","key":"pone.0243418.ref032","first-page":"e3916","article-title":"Purification and visualization of lipopolysaccharide from Gram-negative bacteria by hot aqueous-phenol extraction","author":"MR Davis","year":"2012","journal-title":"JoVE (Journal of Visualized Experiments)"},{"issue":"2","key":"pone.0243418.ref033","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1172\/JCI118423","article-title":"A novel Escherichia coli lipid A mutant that produces an antiinflammatory lipopolysaccharide","volume":"97","author":"JE Somerville","year":"1996","journal-title":"The Journal of clinical investigation"},{"issue":"1","key":"pone.0243418.ref034","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1186\/1471-2180-10-125","article-title":"Pseudomonas aeruginosa inhibits in-vitro Candida biofilm development","volume":"10","author":"H Bandara","year":"2010b","journal-title":"BMC Microbiology"},{"issue":"6","key":"pone.0243418.ref035","doi-asserted-by":"crossref","first-page":"2473","DOI":"10.2353\/ajpath.2009.090166","article-title":"Pseudomonas aeruginosa exotoxin pyocyanin causes cystic fibrosis airway pathogenesis","volume":"175","author":"CC Caldwell","year":"2009","journal-title":"The American journal of pathology"},{"issue":"12","key":"pone.0243418.ref036","doi-asserted-by":"crossref","first-page":"1716","DOI":"10.1111\/cmi.12674","article-title":"Polymicrobial infections involving clinically relevant Gram\u2010negative bacteria and fungi","volume":"18","author":"S Dhamgaye","year":"2016","journal-title":"Cellular microbiology"},{"issue":"21","key":"pone.0243418.ref037","doi-asserted-by":"crossref","first-page":"6454","DOI":"10.1128\/JB.183.21.6454-6465.2001","article-title":"Functional analysis of genes for biosynthesis of pyocyanin and phenazine-1-carboxamide from Pseudomonas aeruginosa PAO1","volume":"183","author":"DV Mavrodi","year":"2001","journal-title":"Journal of bacteriology"},{"issue":"5576","key":"pone.0243418.ref038","doi-asserted-by":"crossref","first-page":"2229","DOI":"10.1126\/science.1070784","article-title":"Pseudomonas-Candida interactions: an ecological role for virulence factors","volume":"296","author":"DA Hogan","year":"2002","journal-title":"Science (New York, NY)"},{"issue":"2","key":"pone.0243418.ref039","first-page":"458","article-title":"Rapid identification of 4-hydroxy-2-alkylquinolines produced by Pseudomonas aeruginosa using gas chromatography\u2014electron-capture mass spectrometry. Journal of Chromatography B","volume":"664","author":"GW Taylor","year":"1995","journal-title":"Biomedical Sciences and Applications"},{"key":"pone.0243418.ref040","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1007\/978-981-10-6141-7_16","volume-title":"Pathophysiological Aspects of Proteases","author":"ACM Galdino","year":"2017"},{"key":"pone.0243418.ref041","doi-asserted-by":"crossref","first-page":"9936","DOI":"10.1038\/srep09936","article-title":"Mechanistic insights into elastin degradation by pseudolysin, the major virulence factor of the opportunistic pathogen Pseudomonas aeruginosa","volume":"5","author":"J Yang","year":"2015","journal-title":"Scientific reports"},{"issue":"11","key":"pone.0243418.ref042","doi-asserted-by":"crossref","first-page":"e27091","DOI":"10.1371\/journal.pone.0027091","article-title":"Pseudomonas aeruginosa elastase provides an escape from phagocytosis by degrading the pulmonary surfactant protein-A","volume":"6","author":"Z Kuang","year":"2011","journal-title":"PloS one"},{"issue":"4","key":"pone.0243418.ref043","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1139\/cjm-2013-0667","article-title":"Elastase LasB of Pseudomonas aeruginosa promotes biofilm formation partly through rhamnolipid-mediated regulation","volume":"60","author":"H Yu","year":"2014","journal-title":"Canadian journal of microbiology"},{"key":"pone.0243418.ref044","article-title":"Molecular and phenotypic study of virulence genes in a pathogenic strain of Pseudomonas aeruginosa isolated from various clinical origins by PCR: profiles of genes and toxins","author":"A Almarzoqi","year":"2015"},{"issue":"5","key":"pone.0243418.ref045","first-page":"274","article-title":"Genetic identification of Pseudomonas aeruginosa virulence genes among different isolates","volume":"7","author":"M Khattab","year":"2015","journal-title":"J Microb Biochem Technol"},{"issue":"5","key":"pone.0243418.ref046","doi-asserted-by":"crossref","first-page":"2371","DOI":"10.1128\/IAI.67.5.2371-2376.1999","article-title":"Pseudomonas aeruginosa hemolytic phospholipase C suppresses neutrophil respiratory burst activity","volume":"67","author":"LS Terada","year":"1999","journal-title":"Infection and Immunity"},{"issue":"4","key":"pone.0243418.ref047","doi-asserted-by":"crossref","first-page":"1234","DOI":"10.1099\/mic.0.033985-0","article-title":"Candida albicans sphingolipid C9-methyltransferase is involved in hyphal elongation","volume":"156","author":"T Oura","year":"2010","journal-title":"Microbiology"},{"issue":"14","key":"pone.0243418.ref048","doi-asserted-by":"crossref","first-page":"9503","DOI":"10.1074\/jbc.274.14.9503","article-title":"Pseudomonas aeruginosa exoenzyme S, a double ADP-ribosyltransferase, resembles vertebrate mono-ADP-ribosyltransferases","volume":"274","author":"AK Ganesan","year":"1999","journal-title":"The Journal of biological chemistry"},{"issue":"9","key":"pone.0243418.ref049","doi-asserted-by":"crossref","first-page":"1314","DOI":"10.1128\/EC.00138-09","article-title":"Glucose Sensing Network in Candida albicans: a Sweet Spot for Fungal Morphogenesis","volume":"8","author":"J Sabina","year":"2009","journal-title":"Eukaryotic Cell"},{"issue":"3","key":"pone.0243418.ref050","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1046\/j.1365-2958.2001.02672.x","article-title":"Ras links cellular morphogenesis to virulence by regulation of the MAP kinase and cAMP signalling pathways in the pathogenic fungus Candida albicans","volume":"42","author":"E Leberer","year":"2001","journal-title":"Molecular microbiology"},{"issue":"357","key":"pone.0243418.ref051","article-title":"Increased Virulence of Bloodstream Over Peripheral Isolates of P. aeruginosa Identified Through Post-transcriptional Regulation of Virulence Factors","volume":"8","author":"C Hickey","year":"2018","journal-title":"Frontiers in Cellular and Infection Microbiology"},{"issue":"5","key":"pone.0243418.ref052","doi-asserted-by":"crossref","first-page":"1212","DOI":"10.1111\/j.1365-2958.2004.04349.x","article-title":"A Pseudomonas aeruginosa quorum-sensing molecule influences Candida albicans morphology","volume":"54","author":"DA Hogan","year":"2004","journal-title":"Mol Microbiol"},{"issue":"Pt 10","key":"pone.0243418.ref053","doi-asserted-by":"crossref","first-page":"1225","DOI":"10.1099\/jmm.0.021832-0","article-title":"Bacterial lipopolysaccharides variably modulate in vitro biofilm formation of Candida species","volume":"59","author":"HM Bandara","year":"2010a","journal-title":"Journal of medical microbiology"},{"issue":"Pt 12","key":"pone.0243418.ref054","doi-asserted-by":"crossref","first-page":"1623","DOI":"10.1099\/jmm.0.012989-0","article-title":"Escherichia coli and its lipopolysaccharide modulate in vitro Candida biofilm formation","volume":"58","author":"HM Bandara","year":"2009","journal-title":"Journal of medical microbiology"},{"issue":"1","key":"pone.0243418.ref055","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1111\/omi.12006","article-title":"Pseudomonas aeruginosa lipopolysaccharide inhibits Candida albicans hyphae formation and alters gene expression during biofilm development","volume":"28","author":"K Bandara HMHN","year":"2013","journal-title":"Molecular Oral Microbiology"},{"key":"pone.0243418.ref056","first-page":"544","article-title":"Oxidative responses and defense mechanism of hyperpigmented P","volume":"17","author":"C Thippakorn","year":"2018","journal-title":"aeruginosa as characterized by proteomics and metabolomics. 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