{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T03:01:13Z","timestamp":1787022073750,"version":"build-2736575974"},"reference-count":45,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2020,12,29]],"date-time":"2020-12-29T00:00:00Z","timestamp":1609200000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:sec id=\"sec001\">\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Reduced antiplatelet activity of aspirin (ALR) or clopidogrel (CLR) is associated with an increased risk of thromboembolic events. The reported prevalence data for low-responders vary widely and there have been few investigations in vascular surgery patients even though they are at high risk for thromb-embolic complications. The aim of this prospective observational monocentric study was to elucidate possible changes in ALR or CLR after common vascular procedures.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec002\">\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Activity of aspirin and clopidogrel was measured by impedance aggregometry using a multiple electrode aggregometer (Multiplate\u00ae). Possible risk factors for ALR or CLR were identified by demographical, clinical data and laboratory parameters. In addition, a follow-up aggregometry was performed after completion of the vascular procedure to identify changes in antiplatelet response.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec003\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>A total of 176 patients taking antiplatelet medications aspirin and\/or clopidogrel with peripheral artery disease (PAD) and\/or carotid stenosis (CS) were included in the study. The prevalence of ALR was 13.1% and the prevalence of CLR was 32% in the aggregometry before vascular treatment. Potential risk factors identified in the aspirin group were concomitant insulin medication (p = 0.0006) and elevated C-reactive protein (CRP) (p = 0.0021). The overall ALR increased significantly postoperatively to 27.5% (p = 0.0006); however, there was no significant change in CLR that was detected. In a subgroup analysis elevation of the platelet count was associated with a post-procedure increase of ALR incidence.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"sec004\">\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The incidence of ALR in vascular surgery patients increases after vascular procedures. An elevated platelet count was detected as a risk factor. Further studies are necessary to analyse this potential influence on patency rates of vascular reconstructions.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1371\/journal.pone.0244330","type":"journal-article","created":{"date-parts":[[2020,12,29]],"date-time":"2020-12-29T13:43:03Z","timestamp":1609249383000},"page":"e0244330","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":7,"title":["Perioperative changes of response to antiplatelet medication in vascular surgery patients"],"prefix":"10.1371","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6931-5930","authenticated-orcid":true,"given":"Thomas","family":"Hummel","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saskia Hannah","family":"Meves","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Breuer-Kaiser","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan-Ole","family":"D\u00fcsterwald","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominic","family":"M\u00fchlberger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Achim","family":"Mumme","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Horst","family":"Neubauer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2020,12,29]]},"reference":[{"issue":"9","key":"pone.0244330.ref001","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1093\/eurheartj\/ehx095","article-title":"2017 ESC Guidelines on the Diagnosis and Treatment of Peripheral Arterial Diseases, in collaboration with the European Society for Vascular Surgery (ESVS).","volume":"39","author":"V Aboyans","year":"2018","journal-title":"Eur Heart J"},{"key":"pone.0244330.ref002","doi-asserted-by":"crossref","first-page":"2888","DOI":"10.1161\/CIRCULATIONAHA.105.596627","article-title":"Residual arachidonic acid-induced platelet activation via an adenosine diphosphate-dependent but cyclooxygenase-1 and cyclooxygenase 2-independent pathway. A 700-patient study of aspirin resistance","volume":"113","author":"AL Frelinger","year":"2006","journal-title":"Circulation"},{"key":"pone.0244330.ref003","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/j.ahj.2006.10.040","article-title":"Prevalence of persistent platelet reactivity despite use of aspirin: a systematic review","volume":"153","author":"MM Hovens","year":"2007","journal-title":"Am Heart J"},{"issue":"4","key":"pone.0244330.ref004","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1080\/AC.67.4.2170686","article-title":"The prevalence and factors associated with aspirin resistance in patients premedicated with aspirin.","volume":"67","author":"SI Al-Azzam","year":"2012","journal-title":"Acta cardiol"},{"issue":"2","key":"pone.0244330.ref005","first-page":"CD000535","article-title":"Antiplatelet agents for preventing thrombosis after peripheral arterial bypass surgery","volume":"2015","author":"R Bedenis","year":"2015","journal-title":"Cochrane Database Syst Rev"},{"key":"pone.0244330.ref006","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/09537104.2020.1742314","article-title":"Impact of high on-treatment platelet reactivity after angioplasty in patients with peripheral arterial disease.","volume":"7","author":"L Busch","year":"2020","journal-title":"Platelets"},{"issue":"5","key":"pone.0244330.ref007","first-page":"446","article-title":"Prevalence of acetylsalicylic acid (ASA)\u2014low response in vascular surgery.","volume":"87","author":"T Hummel","year":"2016","journal-title":"Chirurg"},{"issue":"6","key":"pone.0244330.ref008","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1016\/0049-3848(89)90136-9","article-title":"Transient aggregation resistance of human blood platelets in fresh plasma. I. Method-ology.","volume":"54","author":"MR Hardeman","year":"1989","journal-title":"Thromb Res"},{"issue":"3","key":"pone.0244330.ref009","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1016\/0049-3848(86)90011-3","article-title":"Storage of plate-lets for tests of platelet function: effects of temperature on platelet aggregation, platelet morphology and liberation of beta-thromboglobulin","volume":"44","author":"SE Watts","year":"1986","journal-title":"Thromb Res"},{"issue":"5","key":"pone.0244330.ref010","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1093\/ajcp\/92.5.676","article-title":"Whole blood aggregometry. Influence of sample collection and delay in study performance on test results","volume":"92","author":"D Sweeney J","year":"1989","journal-title":"Am J Clin Pathol"},{"issue":"4","key":"pone.0244330.ref011","doi-asserted-by":"crossref","first-page":"786","DOI":"10.3174\/ajnr.A0917","article-title":"Monitoring of clopidogrel-related platelet inhibition: correlation of nonresponse with clinical outcome in supra-aortic stenting.","volume":"29","author":"S Muller-Schunk","year":"2008","journal-title":"Am J Neuroradiol"},{"issue":"6","key":"pone.0244330.ref012","doi-asserted-by":"crossref","first-page":"1791","DOI":"10.1213\/ane.0b013e3181865733","article-title":"An evaluation of cyclooxygenase-1 inhibition before coronary artery surgery: aggregometry versus patient self-reporting","volume":"107","author":"N Rahe-Meyer","year":"2008","journal-title":"Anesth Analg"},{"issue":"6","key":"pone.0244330.ref013","first-page":"781","article-title":"Multiple electrode aggregometry: a new device to measure platelet aggregation in whole blood","volume":"96","author":"O Toth","year":"2006","journal-title":"Thromb Haemost"},{"issue":"6","key":"pone.0244330.ref014","doi-asserted-by":"crossref","first-page":"1798","DOI":"10.1213\/ane.0b013e31818524c1","article-title":"Point-of-care whole blood impedance aggregometry ver-sus classical light transmission aggregometry for detecting aspirin and clopidogrel: the results of a pilot study","volume":"107","author":"C Velik-Salchner","year":"2008","journal-title":"Anesth Analg"},{"issue":"10","key":"pone.0244330.ref015","doi-asserted-by":"crossref","first-page":"849","DOI":"10.1016\/j.jacc.2008.11.030","article-title":"Platelet reactivity after clopidogrel treatment assessed with point-of-care analysis and early drug-eluting stent thrombosis","volume":"53","author":"D Sibbing","year":"2009","journal-title":"J Am Coll Cardiol"},{"issue":"15","key":"pone.0244330.ref016","doi-asserted-by":"crossref","first-page":"1593","DOI":"10.1001\/archinte.167.15.1593","article-title":"Association of laboratory-defined aspirin resistance with a higher risk of recurrent cardiovascular events\u2013A systematic review and meta-analysis","volume":"167","author":"JD Snoep","year":"2007","journal-title":"Arch Intern Med"},{"key":"pone.0244330.ref017","doi-asserted-by":"crossref","first-page":"1702","DOI":"10.1093\/eurheartj\/ehm226","article-title":"A comparison of six major platelet function tests to determine the prevalence of aspirin resistance in patients with stable coronary artery disease","volume":"28","author":"M Lordkipanidz\u00e9","year":"2007","journal-title":"Eur Heart J"},{"issue":"3","key":"pone.0244330.ref018","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1111\/j.1538-7836.2007.02378.x","article-title":"Mooe, T. Platelet aggregation and aspirin non-responsiveness increase when an acute coronary syndrome is complicated by an infection","volume":"5","author":"A Modica","year":"2007","journal-title":"J Thromb Haemost"},{"issue":"8","key":"pone.0244330.ref019","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1097\/MBC.0b013e328201c77e","article-title":"Inflammatory changes during the \u2018common cold\u2019 are associated with platelet activation and increased reactivity of platelets to agonists.","volume":"18","author":"RP Kreutz","year":"2007","journal-title":"Blood Coagul Fibrinolysis"},{"issue":"5","key":"pone.0244330.ref020","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1111\/j.1742-7843.2006.pto_343.x","article-title":"Reduced blood platelet sensitivity to aspirin in coronary artery disease: are dyslipidaemia and inflammatory states possible factors predisposing to sub-optimal platelet response to aspirin?","volume":"98","author":"L Markuszewski","year":"2006","journal-title":"Basic Clin Pharmacol Toxicol"},{"key":"pone.0244330.ref021","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1111\/j.1365-2141.2004.04983.x","article-title":"Platelet degranulation and monocyte-platelet complex formation are increased in the acute and convalescent phases after ischaemic stroke or transient ischaemic attack","volume":"125","author":"DJ McCabe","year":"2004","journal-title":"Br J Haematol"},{"key":"pone.0244330.ref022","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1111\/j.1749-6632.2010.05733.x","article-title":"Platelet-leukocyte interactions link inflammatatory and thrombo-embolic events in ischemic stroce.","volume":"1207","author":"ZG Franks","year":"2010","journal-title":"Ann N Y Acad Aci"},{"key":"pone.0244330.ref023","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.thromres.2011.08.019","article-title":"Platelet aggregation is dependent on platelet count in patients with coronary artery disease","volume":"129","author":"M W\u00fcrtz","year":"2012","journal-title":"Thromb Res"},{"key":"pone.0244330.ref024","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/j.amjcard.2009.03.022","article-title":"Assessment, Mechanisms, and Clinical Implication of Variability in Platelet Response to Aspirin and Clopidogrel Therapy.","volume":"104","author":"I Ben-Dor","year":"2009","journal-title":"Am J Cardiol"},{"issue":"4","key":"pone.0244330.ref025","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1093\/eurheartj\/ehn562","article-title":"Interindividual variability in the response to oral antiplatelet drugs: a position paper of the Working Group on antiplatelet drugs resistance appointed by the Section of Cardiovascular Interventions of the Polish Cardiac Society, endorsed by the Working Group on Thrombosis of the European Society of Cardiology","volume":"30","author":"W Kuliczkowski","year":"2009","journal-title":"Eur Heart J"},{"key":"pone.0244330.ref026","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.phrs.2013.04.010","article-title":"Homocysteine is a novel risk factor for suboptimal response of blood platelets to acetylsalicylic acid in coronary artery disease: a randomized multicenter study","volume":"74","author":"K Karolczak","year":"2013","journal-title":"Pharmacol Res"},{"key":"pone.0244330.ref027","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1160\/TH03-12-0758","article-title":"Platelet inhibition by aspirin is diminished in patients during carotid surgery: a form of transient aspirin resistance","volume":"92","author":"DA Payne","year":"2004","journal-title":"Thromb Haemost"},{"key":"pone.0244330.ref028","doi-asserted-by":"crossref","first-page":"2028","DOI":"10.1111\/j.1538-7836.2007.02694.x","article-title":"Platelet activation, myocardial ischemic events and postoperative non-response to aspirin in patients undergoing major vascular surgery","volume":"5","author":"S Rajagopalan","year":"2007","journal-title":"J Thromb Haemost"},{"key":"pone.0244330.ref029","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1097\/00003643-201306001-00204","article-title":"Postoperative low aspirin response in vascular surgery: a pilot study: 4AP4\u20109","volume":"30","author":"F Hagedorn","year":"2013","journal-title":"Eur J Anaest"},{"key":"pone.0244330.ref030","doi-asserted-by":"crossref","first-page":"1366","DOI":"10.1182\/blood.V71.5.1366.1366","article-title":"Shearinduced platelet aggregation can be mediated by vWF released from platelets, as well as by exogenous large or unusually large vWF multimers, requires adenosine diphosphate, and is resistant to aspirin","volume":"71","author":"JL Moake","year":"1988","journal-title":"Blood"},{"key":"pone.0244330.ref031","doi-asserted-by":"crossref","first-page":"652","DOI":"10.1160\/TH05-10-0653","article-title":"Dose- and time-dependent antiplatelet effects of aspirin","volume":"95","author":"C Perneby","year":"2006","journal-title":"Thromb Haemost"},{"key":"pone.0244330.ref032","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1016\/j.jacc.2008.05.031","article-title":"Role of reticu-lated platelets and platelet size heterogeneity on platelet activity after dual an-tiplatelet therapy with aspirin and clopidogrel in patients with stable coronary artery disease","volume":"52","author":"S Guthikonda","year":"2008","journal-title":"J Am Coll Cardiol"},{"issue":"6","key":"pone.0244330.ref033","doi-asserted-by":"crossref","first-page":"918","DOI":"10.1002\/cyto.b.21642","article-title":"Platelet characteristics in patients with essential thrombocytosis.","volume":"94","author":"OH Pedersen","year":"2018","journal-title":"Cytometry B Clin Cytom."},{"key":"pone.0244330.ref034","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1186\/1741-7015-9-3","article-title":"Tailored antiplatelet therapy can overcome clopidogrel and aspirin resistance\u2013The BOchum CLopidogrel and Aspirin Plan (BOCLA-Plan) to improve antiplatelet therapy.","volume":"9","author":"H Neubauer","year":"2011","journal-title":"BMC Med"},{"key":"pone.0244330.ref035","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1136\/bmj.39430.529549.BE","article-title":"Aspirin \u201cresistance\u201d and risk of cardiovascular morbidity: systemic review and meta-analyses.","volume":"336","author":"G Krasopoulos","year":"2008","journal-title":"BMJ"},{"key":"pone.0244330.ref036","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1160\/TH11-01-0040","article-title":"High platelet reactivity and clinical outcome\u2013Fact and fiction","volume":"106","author":"D Sibbing","year":"2011","journal-title":"Thromb Haemost"},{"key":"pone.0244330.ref037","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/j.ahj.2007.04.014","article-title":"Clopidogrel nonresponsiveness in patients undergoing percutaneous coronary intervention with stenting: a systematic review and meta-analyses","volume":"154","author":"JD Snoep","year":"2007","journal-title":"Am Heart J"},{"key":"pone.0244330.ref038","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1160\/TH08-01-0056","article-title":"Clinical implications of aspirin resistance","volume":"100","author":"N Zimmermann","year":"2008","journal-title":"Thromb Haemost"},{"key":"pone.0244330.ref039","doi-asserted-by":"crossref","first-page":"1339","DOI":"10.1016\/j.amjcard.2009.01.341","article-title":"Predictors of Heightened Platelet Reactivity Despite Dual-Antiplatelet Therapy in Patients Undergoing Percutaneous Coronary Intervention.","volume":"103","author":"MJ Price","year":"2009","journal-title":"Am J Cardiol"},{"issue":"2","key":"pone.0244330.ref040","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1024\/0301-1526\/a000510","article-title":"High on-treatment platelet reactivity and outcomes after percutaneous endovascular procedures in patients with peripheral artery disease","volume":"45","author":"I Bernlochner","year":"2016","journal-title":"Vasa"},{"key":"pone.0244330.ref041","doi-asserted-by":"crossref","first-page":"2428","DOI":"10.1016\/j.jacc.2013.03.036","article-title":"Platelet responsiveness to clopidogrel treatment after peripheral endovascular procedures: the PRECLOP study: clinical impact and optimal cutoff value of on-treatment high platelet reactivity","volume":"61","author":"S Spiliopoulos","year":"2013","journal-title":"J Am Coll Cardiol"},{"key":"pone.0244330.ref042","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1186\/1479-5876-6-46","article-title":"Non-compliance is the predominant cause of aspirin resistance in chronic coronary arterial disease patients.","volume":"6","author":"KA Schwartz","year":"2008","journal-title":"J Transl Med"},{"issue":"5","key":"pone.0244330.ref043","doi-asserted-by":"crossref","first-page":"598","DOI":"10.1001\/jamacardio.2020.0116","article-title":"Rationale and Design of the Aspirin 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