{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T03:54:11Z","timestamp":1788926051088,"version":"build-2803163510"},"reference-count":43,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2020,12,29]],"date-time":"2020-12-29T00:00:00Z","timestamp":1609200000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000987","name":"Melbourne Research, University of Melbourne","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000987","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012504","name":"Royal Melbourne Hospital Neuroscience Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100012504","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100015572","name":"National Imaging Facility","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100015572","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>The default mode network (DMN) is the main large-scale network of the resting brain and the PCC\/precuneus is a major hub of this network. Glutamate and GABA (\u03b3-amino butyric acid) are the main excitatory and inhibitory neurotransmitters in the CNS, respectively. We studied glutamate and GABA concentrations in the PCC\/precuneus via magnetic resonance spectroscopy (MRS) at 7T in relation to age and correlated them with functional connectivity between this region and other DMN nodes in ten healthy right-handed volunteers ranging in age between 23\u201368 years. Mean functional connectivity of the PCC\/precuneus to the other DMN nodes and the glutamate\/GABA ratio significantly correlated with age (<jats:italic>r<\/jats:italic> = 0.802, <jats:italic>p<\/jats:italic> = 0.005 and <jats:italic>r<\/jats:italic> = 0.793, <jats:italic>p<\/jats:italic> = 0.006, respectively) but not with each other. Glutamate and GABA alone did not significantly correlate with age nor with functional connectivity within the DMN. The glutamate\/GABA ratio and functional connectivity of the PCC\/precuneus are, therefore, independent age-related biomarkers of the DMN and may be combined in a multimodal pipeline to study DMN alterations in various disease states.<\/jats:p>","DOI":"10.1371\/journal.pone.0244491","type":"journal-article","created":{"date-parts":[[2020,12,29]],"date-time":"2020-12-29T19:01:20Z","timestamp":1609268480000},"page":"e0244491","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":11,"title":["Resting-state functional connectivity and quantitation of glutamate and GABA of the PCC\/precuneus by magnetic resonance spectroscopy at 7T in healthy individuals"],"prefix":"10.1371","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3833-9128","authenticated-orcid":true,"given":"Ofer M.","family":"Gonen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bradford A.","family":"Moffat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Kwan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Terence J.","family":"O\u2019Brien","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia M.","family":"Desmond","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elaine","family":"Lui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2020,12,29]]},"reference":[{"key":"pone.0244491.ref001","article-title":"Common blood flow changes across visual tasks: II. 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