{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T06:53:07Z","timestamp":1790751187502,"version":"4.1.0"},"reference-count":31,"publisher":"Public Library of Science (PLoS)","issue":"12","license":[{"start":{"date-parts":[[2020,12,31]],"date-time":"2020-12-31T00:00:00Z","timestamp":1609372800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100002156","name":"Israel Cancer Association USA","doi-asserted-by":"publisher","award":["8037815"],"award-info":[{"award-number":["8037815"]}],"id":[{"id":"10.13039\/100002156","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:sec id=\"sec001\">\n<jats:title>Objectives<\/jats:title>\n<jats:p>To compare hematologic and serological parameters among patients with Sjogren\u2019s syndrome (SS), dry eye syndrome (DES) and controls, and validate a novel multiplex-serology method for identifying auto-antibodies in these populations.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec002\">\n<jats:title>Methods<\/jats:title>\n<jats:p>In a clinic-based case-control study a total of 422 participants were recruited, including 91 with SS, 120 DES, and 211 controls (age and sex frequency-matched). We measured blood counts, anti-nuclear-antibodies (ANA), anti-SSA\/SSB, anti-ribonucleoprotein (RNP), anti-double-stranded-DNA (DS-DNA), and rheumatoid factor (RF) using the \u201c<jats:italic>Immunodot\u201d<\/jats:italic> qualitative-ELISA assay. Immunoglobulins, C3 and C4 were measured by immune-fluorescence. Autoantibodies were also quantified with a newly-developed method using glutathione-S-transferase fusion proteins of SSA\/Ro 52 and 60kD and SSB\/La (multiplex-serology), measuring median fluorescence intensity (MFI).<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec003\">\n<jats:title>Results<\/jats:title>\n<jats:p>Among DES patients, only 2% (95%CI: 0.36\u20136.3) had positive immune serology. SS patients had lower lymphocyte, hemoglobin and C3 levels but higher prevalence of RF, ANA, anti-SSA\/B and higher IgG and MFI levels, compared to DES and controls (P&lt;0.001). Presence of anti-SSA\/Ro-52kD was associated with SS [odds ratio (OR) = 2.05, 95% confidence interval (CI): 1.46\u20132.88]. Anti-SSB\/La was inversely associated with DES (OR = 0.81, 95%CI: 0.65\u20131.00) compared to controls. Positivity to RF (adjusted for age, gender and ethnicity OR = 5.03, 95%CI: 1.78\u201314.21), ANA (OR = 14.75, 95%CI: 4.09\u201353.17), or combination of anti-SSA\/B (OR = 20.97, 95%CI: 4.60\u201395.54) were more likely in SS compared to DES. The novel multiplex-serology method correctly identified anti-SSA\/B autoantibodies by ELISA among SS, DES patients and controls (sensitivity = 1.0, negative-predictive-value = 1.0).<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec004\">\n<jats:title>Conclusions<\/jats:title>\n<jats:p>Serologic parameters distinguish SS from DES patients and controls. A newly-developed multiplex-serology technique may be useful to detect autoantibodies in large epidemiologic studies.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pone.0244712","type":"journal-article","created":{"date-parts":[[2020,12,31]],"date-time":"2020-12-31T19:26:12Z","timestamp":1609442772000},"page":"e0244712","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":3,"title":["Serological and hematological characteristics of Sjogren\u2019s syndrome and dry eye syndrome patients using a novel immune serology technique"],"prefix":"10.1371","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2832-4221","authenticated-orcid":true,"given":"Hadas","family":"Ben-Eli","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abraham","family":"Solomon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Doron J.","family":"Aframian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eldad","family":"Ben-Chetrit","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dror","family":"Mevorach","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geffen","family":"Kleinstern","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tim","family":"Waterboer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martina","family":"Willhauck-Fleckenstein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4720-8306","authenticated-orcid":true,"given":"Michael","family":"Pawlita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ora","family":"Paltiel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2020,12,31]]},"reference":[{"issue":"1","key":"pone.0244712.ref001","doi-asserted-by":"crossref","DOI":"10.1136\/bmjopen-2015-009675","article-title":"Prevalence of dry eye disease in visual display terminal workers: a systematic review and meta-analysis","volume":"6","author":"R Courtin","year":"2016","journal-title":"BMJ Open"},{"key":"pone.0244712.ref002","unstructured":"The definition and classification of dry eye disease: report of the Definition and Classification Subcommittee of the International Dry Eye WorkShop (2007). 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