{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T07:06:49Z","timestamp":1792220809181,"version":"4.4.0"},"reference-count":26,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2021,1,14]],"date-time":"2021-01-14T00:00:00Z","timestamp":1610582400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development","award":["R03 HD101765"],"award-info":[{"award-number":["R03 HD101765"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Genome association studies in human and genetic studies in mouse implicated members of the transmembrane protein 132 (TMEM132) family in multiple conditions including panic disorder, hearing loss, limb and kidney malformation. However, the presence of five TMEM132 paralogs in mammalian genomes makes it extremely challenging to reveal the full requirement for these proteins in vivo. In contrast, there is only one TMEM132 homolog, <jats:italic>detonator<\/jats:italic> (<jats:italic>dtn<\/jats:italic>), in the genome of fruit fly <jats:italic>Drosophila melanogaster<\/jats:italic>, enabling straightforward research into its <jats:italic>in vivo<\/jats:italic> function. In the current study, we generate multiple loss-of-function <jats:italic>dtn<\/jats:italic> mutant fly strains through a polycistronic tRNA-gRNA approach, and show that most embryos lacking both maternal and paternal dtn fail to hatch into larvae, indicating an essential role of <jats:italic>dtn<\/jats:italic> in <jats:italic>Drosophila<\/jats:italic> reproduction.<\/jats:p>","DOI":"10.1371\/journal.pone.0245454","type":"journal-article","created":{"date-parts":[[2021,1,14]],"date-time":"2021-01-14T19:13:43Z","timestamp":1610651623000},"page":"e0245454","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":6,"title":["Efficient multiplexed genome engineering with a polycistronic tRNA and CRISPR guide-RNA reveals an important role of detonator in reproduction of Drosophila 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