{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T13:13:44Z","timestamp":1786626824217,"version":"build-2736575974"},"reference-count":17,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2021,1,15]],"date-time":"2021-01-15T00:00:00Z","timestamp":1610668800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"the Japan Society for the Promotion of Science","doi-asserted-by":"crossref","award":["JP16K15535, JP17H05294, JP18K07876 and JP26220004"],"award-info":[{"award-number":["JP16K15535, JP17H05294, JP18K07876 and JP26220004"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Some human organs are composed of bifurcated structures. Two simple branching modes\u2014monopodial and dipodial\u2014have been proposed. With monopodial branching, child branches extend from the sidewall of the parent branch. With dipodial branching, the tip of the bronchus bifurcates. However, the branching modes of the human bronchial tree have not been elucidated precisely. A total of 48 samples between Carnegie stage (CS) 15 and CS23 belonging to the Kyoto Collection were used to acquire imaging data with phase-contrast X-ray computed tomography. Bronchial trees of all samples were three-dimensionally reconstructed from the image data. We analyzed the lobar bronchus, segmental bronchus, and subsegmental bronchus. After calculating each bronchus length, we categorized the branching mode of the analyzed bronchi based on whether the parent bronchus was divided after generation of the analyzed bronchi. All lobar bronchi were formed with monopodial branching. Twenty-five bifurcations were analyzed to categorize the branching mode of the segmental and subsegmental bronchi; 22 bifurcations were categorized as monopodial branching, two bifurcations were not categorized as any branching pattern, and the only lingular bronchus that bifurcated from the left superior lobar bronchus was categorized as dipodial branching. The left superior lobar bronchus did not shorten during the period from CS17 or CS18, when the child branch was generated, to CS23. All analyzed bronchi that could be categorized, except for one, were categorized as monopodial branching. The branching modes of the lobar bronchus and segmental bronchus were similar in the mouse lung and human lung; however, the modes of the subsegmental bronchi were different. Furthermore, remodeling, such as shrinkage of the bronchus, was not observed during the analysis period. Our three-dimensional reconstructions allowed precise calculation of the bronchus length, thereby improving the knowledge of branching morphogenesis in the human embryonic lung.<\/jats:p>","DOI":"10.1371\/journal.pone.0245558","type":"journal-article","created":{"date-parts":[[2021,1,15]],"date-time":"2021-01-15T18:30:01Z","timestamp":1610735401000},"page":"e0245558","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":11,"title":["Bronchial tree of the human embryo: Categorization of the branching mode as monopodial and dipodial"],"prefix":"10.1371","volume":"16","author":[{"given":"Sena","family":"Fujii","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taiga","family":"Muranaka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Matsubayashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigehito","family":"Yamada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6925-6723","authenticated-orcid":true,"given":"Akio","family":"Yoneyama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9660-9384","authenticated-orcid":true,"given":"Tetsuya","family":"Takakuwa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2021,1,15]]},"reference":[{"key":"pone.0245558.ref001","first-page":"69","article-title":"Early developmental stages of the human lung","volume":"36","author":"DM Palmer","year":"1936","journal-title":"Ohio J Sci"},{"issue":"7196","key":"pone.0245558.ref002","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1038\/nature07005","article-title":"The branching programme of mouse lung development","volume":"453","author":"RJ Metzger","year":"2008","journal-title":"Nature"},{"issue":"1","key":"pone.0245558.ref003","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/j.ydbio.2004.03.025","article-title":"Real-time analysis of ureteric bud branching morphogenesis in vitro","volume":"271","author":"T Watanabe","year":"2004","journal-title":"Dev Biol"},{"key":"pone.0245558.ref004","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1111\/joa.13199","article-title":"The bronchial tree of the human embryo: an analysis of variations in the bronchial segments","volume":"237","author":"S Fujii","year":"2020","journal-title":"J Anat"},{"key":"pone.0245558.ref005","first-page":"133","article-title":"Developmental horizons in human embryos. 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