{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T14:07:16Z","timestamp":1740146836594,"version":"3.37.3"},"reference-count":22,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2021,1,28]],"date-time":"2021-01-28T00:00:00Z","timestamp":1611792000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["grant #17-16-01099"],"award-info":[{"award-number":["grant #17-16-01099"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>In this article, the method of analysis of GB-speckles (gene-based speckles) has been adapted to the problem of detecting the differences in a group of genes (usually 5\u20137)<jats:strike>,<\/jats:strike> used in Multi Locus Sequence Typing (MLST). This method is based on <jats:italic>s-LASCA<\/jats:italic> imaging (<jats:italic>s<\/jats:italic>patial <jats:italic>La<\/jats:italic>ser <jats:italic>S<\/jats:italic>peckle <jats:italic>C<\/jats:italic>ontrast <jats:italic>A<\/jats:italic>nalysis) of virtual GB-speckle and on the technique of RGB coordinates for GB-speckles<jats:strike>,<\/jats:strike> processed by the <jats:italic>s-LASCA<\/jats:italic> method. A very high sensitivity and accuracy of the new method for detecting gene polymorphism as a great alternative to classical MLST has been demonstrated. The analysis of GB-speckles<jats:strike>,<\/jats:strike> obtained for the concatenated sequences of seven genes (<jats:italic>gatA<\/jats:italic>, <jats:italic>gidA<\/jats:italic>, <jats:italic>enoA<\/jats:italic>, <jats:italic>fumC<\/jats:italic>, <jats:italic>hemN<\/jats:italic>, <jats:italic>hflX<\/jats:italic>, <jats:italic>oppA<\/jats:italic>) of three different <jats:italic>Chlamydia trachomatis<\/jats:italic> strains (E\/Bour, ST94; G\/9301, ST95; G\/11222, ST94) has been applied as the model. The high efficiency of usage of s-<jats:italic>LASCA<\/jats:italic>-imaging of GB-speckles has been shown. The data obtained represent a significant progress in digital biology as a whole and improvements in the bio-digitalization of bacterial DNA.<\/jats:p>","DOI":"10.1371\/journal.pone.0245657","type":"journal-article","created":{"date-parts":[[2021,1,28]],"date-time":"2021-01-28T18:37:15Z","timestamp":1611859035000},"page":"e0245657","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":9,"title":["Could LASCA-imaging of GB-speckles be applied for a high discrimination and typing of pathogenic bacteria?"],"prefix":"10.1371","volume":"16","author":[{"given":"Onega","family":"Ulianova","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3030-6927","authenticated-orcid":true,"given":"Sergey","family":"Ulyanov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1373-8229","authenticated-orcid":true,"given":"Sergey","family":"Zaytsev","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuriy","family":"Saltykov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander","family":"Ulyanov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valentina","family":"Feodorova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2021,1,28]]},"reference":[{"volume-title":"Topics in applied physics","year":"1975","author":"JC Dainty","key":"pone.0245657.ref001"},{"key":"pone.0245657.ref002","first-page":"1","volume-title":"Optical Diagnostics of Biological Fluids and Advanced Techniques in Analytical Cytology","author":"Y Aizu","year":"1997"},{"key":"pone.0245657.ref003","doi-asserted-by":"crossref","first-page":"R35","DOI":"10.1088\/0967-3334\/22\/4\/201","article-title":"Laser doppler, speckle and related techniques for blood perfusion mapping and imaging","volume":"22","author":"DJ Briers","year":"2001","journal-title":"Physiol. 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