{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,18]],"date-time":"2026-02-18T23:54:16Z","timestamp":1771458856425,"version":"3.50.1"},"reference-count":61,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2021,1,22]],"date-time":"2021-01-22T00:00:00Z","timestamp":1611273600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Ministry of Health of the Czech Republic","award":["16-28587A"],"award-info":[{"award-number":["16-28587A"]}]},{"name":"the project PERSONMED \u2013 Center for the Development of Personalized Medicine in Age-Related Diseases","award":["Reg. Nr. CZ.02.1.01\/0.0\/0.0\/ 17_048\/0007441"],"award-info":[{"award-number":["Reg. Nr. 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Bacterial and<jats:italic>G<\/jats:italic>.<jats:italic>vaginalis<\/jats:italic>DNA were assessed in the cervical fluid samples using quantitative PCR technique. Concentrations of interleukin-6 and MIAC were evaluated in the amniotic fluid samples. Loads of<jats:italic>G<\/jats:italic>.<jats:italic>vaginalis<\/jats:italic>DNA \u2265 1% of the total cervical bacterial DNA were used to define the cervical prevalence of<jats:italic>G<\/jats:italic>.<jats:italic>vaginalis<\/jats:italic>as abundant. Based on the MIAC and IAI, women were categorized into four groups: with intra-amniotic infection (both MIAC and IAI), with sterile IAI (IAI without MIAC), with MIAC without IAI, and without either MIAC or IAI.<\/jats:p><\/jats:sec><jats:sec id=\"sec003\"><jats:title>Results<\/jats:title><jats:p>The presence of the abundant cervical<jats:italic>G<\/jats:italic>.<jats:italic>vaginalis<\/jats:italic>was related to MIAC (with: 65% vs. without: 44%;<jats:italic>p<\/jats:italic>= 0.0004) but not IAI (with: 52% vs. without: 48%;<jats:italic>p<\/jats:italic>= 0.70). Women with MIAC without IAI had the highest load of the cervical<jats:italic>G<\/jats:italic>.<jats:italic>vaginalis<\/jats:italic>DNA (median 2.0 \u00d7 10<jats:sup>4<\/jats:sup>copies DNA\/mL) and the highest presence of abundant cervical<jats:italic>G<\/jats:italic>.<jats:italic>vaginalis<\/jats:italic>(73%).<\/jats:p><\/jats:sec><jats:sec id=\"sec004\"><jats:title>Conclusions<\/jats:title><jats:p>In women with PPROM, the presence of cervical<jats:italic>G<\/jats:italic>.<jats:italic>vaginalis<\/jats:italic>was associated with MIAC, mainly without the concurrent presence of IAI.<\/jats:p><\/jats:sec>","DOI":"10.1371\/journal.pone.0245937","type":"journal-article","created":{"date-parts":[[2021,1,22]],"date-time":"2021-01-22T22:39:32Z","timestamp":1611355172000},"page":"e0245937","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":19,"title":["Cervical Gardnerella vaginalis in women with preterm prelabor rupture 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