{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T14:33:21Z","timestamp":1788878001916,"version":"build-2803163510"},"reference-count":49,"publisher":"Public Library of Science (PLoS)","issue":"1","license":[{"start":{"date-parts":[[2021,1,27]],"date-time":"2021-01-27T00:00:00Z","timestamp":1611705600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Low-grade gliomas (LGGs) is a primary invasive brain tumor that grows slowly but is incurable and eventually develops into high malignant glioma. Novel biomarkers for the tumorigenesis and lifetime of LGG are critically demanded to be investigated. In this study, the expression levels of procollagen-lysine, 2-oxoglutarate 5-dioxygenases (PLODs) were analyzed by ONCOMINE, HPA and GEPIA. The GEPIA online platform was applied to evaluate the interrelation between PLODs and survival index in LGG. Furthermore, functions of PLODs and co-expression genes were inspected by the DAVID. Moreover, we used TIMER, cBioportal, GeneMINIA and NetworkAnalyst analysis to reveal the mechanism of PLODs in LGG. We found that expression levels of each PLOD family members were up-regulated in patients with LGG. Higher expression of PLODs was closely related to shorter disease-free survival (DFS) and overall survival (OS). The findings showed that LGG cases with or without alterations were significantly correlated with the OS and DFS. The mechanism of PLODs in LGG may be involved in response to hypoxia, oxidoreductase activity, Lysine degradation and immune cell infiltration. In general, this research has investigated the values of PLODs in LGG, which could serve as biomarkers for diagnosis, prognosis and potential therapeutic targets of LGG patients.<\/jats:p>","DOI":"10.1371\/journal.pone.0246097","type":"journal-article","created":{"date-parts":[[2021,1,27]],"date-time":"2021-01-27T13:25:07Z","timestamp":1611753907000},"page":"e0246097","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":8,"title":["Comprehensive analysis of PLOD family members in low-grade gliomas using bioinformatics methods"],"prefix":"10.1371","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9419-6766","authenticated-orcid":true,"given":"Yonghui","family":"Zhao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junchao","family":"Yao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2021,1,27]]},"reference":[{"issue":"4","key":"pone.0246097.ref001","doi-asserted-by":"crossref","first-page":"450","DOI":"10.23736\/S0390-5616.19.04701-5","article-title":"The management of low-grade gliomas in adults","volume":"63","author":"RA Morshed","year":"2019","journal-title":"J Neurosurg Sci"},{"issue":"3","key":"pone.0246097.ref002","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1007\/s10014-016-0265-5","article-title":"Cancer metabolism as a central driving force of glioma pathogenesis","volume":"33","author":"K Masui","year":"2016","journal-title":"Brain Tumor Pathol"},{"key":"pone.0246097.ref003","first-page":"1","article-title":"Molecular and cellular intratumoral heterogeneity in primary glioblastoma: clinical and translational implications","author":"JD Bernstock","year":"2019","journal-title":"J Neurosurg"},{"key":"pone.0246097.ref004","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.canlet.2020.01.027","article-title":"Glioblastoma precision therapy: From the bench to the clinic","volume":"475","author":"Y Zhou","year":"2020","journal-title":"Cancer Lett"},{"issue":"46","key":"pone.0246097.ref005","doi-asserted-by":"crossref","first-page":"36158","DOI":"10.1074\/jbc.M006203200","article-title":"Lysyl hydroxylase 3 is a multifunctional protein possessing collagen glucosyltransferase activity","volume":"275","author":"J Heikkinen","year":"2000","journal-title":"The Journal of biological chemistry"},{"issue":"4","key":"pone.0246097.ref006","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1016\/j.ajhg.2008.09.004","article-title":"A connective tissue disorder caused by mutations of the lysyl hydroxylase 3 gene","volume":"83","author":"AM Salo","year":"2008","journal-title":"Am J Hum Genet"},{"issue":"7","key":"pone.0246097.ref007","doi-asserted-by":"crossref","first-page":"1376","DOI":"10.1002\/jbmr.3692","article-title":"SiMPLOD, a Structure-Integrated Database of Collagen Lysyl Hydroxylase (LH\/PLOD) Enzyme Variants","volume":"34","author":"L Scietti","year":"2019","journal-title":"J Bone Miner Res"},{"issue":"39","key":"pone.0246097.ref008","doi-asserted-by":"crossref","first-page":"14120","DOI":"10.1073\/pnas.0404966101","article-title":"Premature aggregation of type IV collagen and early lethality in lysyl hydroxylase 3 null mice","volume":"101","author":"K Rautavuoma","year":"2004","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"2","key":"pone.0246097.ref009","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1006\/cyto.1999.0539","article-title":"Tumour necrosis factor alpha enhances the expression of hydroxyl lyase, cytoplasmic antiproteinase-2 and a dual specificity kinase TTK in human chondrocyte-like cells","volume":"12","author":"H Ah-Kim","year":"2000","journal-title":"Cytokine"},{"key":"pone.0246097.ref010","doi-asserted-by":"crossref","first-page":"66","DOI":"10.3389\/fcell.2018.00066","article-title":"Roles of PLODs in Collagen Synthesis and Cancer Progression","volume":"6","author":"Y Qi","year":"2018","journal-title":"Front Cell Dev Biol"},{"issue":"2","key":"pone.0246097.ref011","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1593\/neo.07112","article-title":"Oncomine 3.0: genes, pathways, and networks in a collection of 18,000 cancer gene expression profiles","volume":"9","author":"DR Rhodes","year":"2007","journal-title":"Neoplasia"},{"issue":"6220","key":"pone.0246097.ref012","doi-asserted-by":"crossref","first-page":"1260419","DOI":"10.1126\/science.1260419","article-title":"Proteomics. 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