{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T07:34:11Z","timestamp":1767771251536,"version":"3.37.3"},"reference-count":72,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2021,2,2]],"date-time":"2021-02-02T00:00:00Z","timestamp":1612224000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["PI19\/01282"],"award-info":[{"award-number":["PI19\/01282"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012818","name":"Comunidad de Madrid","doi-asserted-by":"publisher","award":["S2013\/ABI-2783, \u201cINSPIRA1-CM\u201d"],"award-info":[{"award-number":["S2013\/ABI-2783, \u201cINSPIRA1-CM\u201d"]}],"id":[{"id":"10.13039\/100012818","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"Fondo Europeo de Desarrollo Regional","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Androgens may exert cardiovascular protective actions by regulating the release and function of different vascular factors. In addition, testosterone (TES) and its 5-reduced metabolites, 5\u03b1- and 5\u03b2-dihydrotestosterone (5\u03b1- and 5\u03b2-DHT) induce vasorelaxant and hypotensive effects. Furthermore, hypertension has been reported to alter the release and function of the neurotransmitters nitric oxide (NO), calcitonin gene-related peptide (CGRP) and noradrenaline (NA). Since the mesenteric arteries possess a dense perivascular innervation and significantly regulate total peripheral vascular resistance, the objective of this study was to analyze the effect of TES, 5\u03b1- and 5\u03b2-DHT on the neurogenic release and vasomotor function of NO, CGRP and NA. For this purpose, the superior mesenteric artery from male spontaneously hypertensive rats (SHR) and normotensive Wistar Kyoto (WKY) rats was used to analyze: (i) the effect of androgens (10 nM, incubated for 30 min) on the neurogenic release of NO, CGRP and NA and (ii) the vasoconstrictor-response to NA and the vasodilator responses to the NO donor, sodium nitroprusside (SNP) and exogenous CGRP. The results showed that TES, 5\u03b1- or 5\u03b2-DHT did not modify the release of NO, CGRP or NA induced by electrical field stimulation (EFS) in the arteries of SHR; however, in the arteries of WKY rats androgens only caused an increase in EFS-induced NO release. Moreover, TES, and especially 5\u03b2-DHT, increased the vasodilator response induced by SNP and CGRP in the arteries of SHR. These findings could be contributing to the hypotensive\/antihypertensive efficacy of 5\u03b2-DHT previously described in conscious SHR and WKY rats, pointing to 5\u03b2- DHT as a potential drug for the treatment of hypertension.<\/jats:p>","DOI":"10.1371\/journal.pone.0246254","type":"journal-article","created":{"date-parts":[[2021,2,2]],"date-time":"2021-02-02T20:19:42Z","timestamp":1612297182000},"page":"e0246254","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":3,"title":["Vasomotor action of androgens in the mesenteric artery of hypertensive rats. Role of perivascular innervation"],"prefix":"10.1371","volume":"16","author":[{"given":"Luc\u00eda","family":"Isidoro-Garc\u00eda","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diva M.","family":"Villalpando","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4670-7146","authenticated-orcid":true,"given":"Mercedes","family":"Ferrer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2021,2,2]]},"reference":[{"key":"pone.0246254.ref001","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/j.niox.2005.02.003","article-title":"Androgens deprivation increases neuronal nitric oxide metabolism and its vasodilatador effect in rat mesenteric arteries","volume":"12","author":"M Mart\u00edn","year":"2005","journal-title":"Nitric Oxide"},{"key":"pone.0246254.ref002","doi-asserted-by":"crossref","first-page":"526","DOI":"10.1159\/000088342","article-title":"Male castration increases neuronal nitric oxide synthase activity in the rat mesenteric artery through protein kinase C activation","volume":"42","author":"J Blanco-Rivero","year":"2005","journal-title":"J Vasc Res"},{"key":"pone.0246254.ref003","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1159\/000098820","article-title":"Orchidectomy increases beta-adrenoceptor activation-mediated neuronal nitric oxide and noradrenaline release in rat mesenteric artery","volume":"84","author":"J Blanco-Rivero","year":"2006","journal-title":"Neuroendocrinology"},{"key":"pone.0246254.ref004","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1093\/cvr\/cvm059","article-title":"Orchidectomy increases the formation of prostanoids and modulates their role in the acetylcholine-induced relaxation in the rat aorta","volume":"77","author":"A Martorell","year":"2008","journal-title":"Cardiovasc Res"},{"key":"pone.0246254.ref005","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1677\/JOE-07-0647","article-title":"Orchidectomy increases the formation of non-endothelial thromboxane A2 and modulates its role in the electrical field stimulation-induced response in rat mesenteric artery","volume":"197","author":"L del Campo","year":"2008","journal-title":"J Endocrinol"},{"issue":"7","key":"pone.0246254.ref006","doi-asserted-by":"crossref","first-page":"e102523","DOI":"10.1371\/journal.pone.0102523","article-title":"Time-dependent effect of orchidectomy on vascular nitric oxide and thromboxane A2 release. 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