{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T03:01:19Z","timestamp":1787022079059,"version":"build-2736575974"},"reference-count":84,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2021,2,2]],"date-time":"2021-02-02T00:00:00Z","timestamp":1612224000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Lovelace Respiratory Research Institute"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>\n                    Prostaglandin E\n                    <jats:sub>2<\/jats:sub>\n                    (PGE\n                    <jats:sub>2<\/jats:sub>\n                    )-induced coughs\n                    <jats:italic>in vivo<\/jats:italic>\n                    and vagal nerve depolarization\n                    <jats:italic>in vitro<\/jats:italic>\n                    are inhibited by systemic and local administration of prostaglandin EP3 receptor (L-798106) and TRPV1 antagonists (JNJ 17203212). These results indicate a modulating effect of TRPV1 on the EP3 receptor-mediated cough responses to PGE\n                    <jats:sub>2<\/jats:sub>\n                    likely through the vagal sensory nerve. This study aimed to determine whether 1) inhalation of aerosolized JNJ 17203212 and L-798106 affected cough responses to citric acid (CA, mainly stimulating TRPV1) and PGE\n                    <jats:sub>2<\/jats:sub>\n                    ; 2) TRPV1 and EP3 receptor morphologically are co-expressed and electrophysiologically functioned in the individual of vagal pulmonary C-neurons (cell bodies of bronchopulmonary C-fibers in the nodose\/jugular ganglia); and 3) there was a cross-effect of TRPV1 and EP3 receptor on these neural excitations. To this end, aerosolized CA or PGE\n                    <jats:sub>2<\/jats:sub>\n                    was inhaled by unanesthetized guinea pigs pretreated without or with each antagonist given in aerosol form. Immunofluorescence was applied to identify the co-expression of TRPV1 and EP3 receptor in vagal pulmonary C-neurons (retrogradely traced by DiI). Whole-cell voltage patch clamp approach was used to detect capsaicin (CAP)- and PGE\n                    <jats:sub>2<\/jats:sub>\n                    -induced currents in individual vagal pulmonary C-neurons and determine the effects of the TRPV1 and EP3 receptor antagonists on the evoked currents. We found that PGE\n                    <jats:sub>2<\/jats:sub>\n                    -induced cough was attenuated by JNJ 17203212 or L-798106 and CA-evoked cough greatly suppressed only by JNJ 17203212. Approximately 1\/4 of vagal pulmonary C-neurons co-expressed EP3 with a cell size &lt; 20 \u03bcm. Both CAP- and PGE\n                    <jats:sub>2<\/jats:sub>\n                    -induced currents could be recorded in the individuals of some vagal pulmonary C-neurons. The former was largely inhibited only by JNJ 17203212, while the latter was suppressed by JNJ 17203212 or L-798106. The similarity of the cross-effect of both antagonists on cough and vagal pulmonary C-neural activity suggests that a subgroup of vagal pulmonary C-neurons co-expressing TRPV1 and EP3 receptor is, at least in part, responsible for the cough response to PGE\n                    <jats:sub>2<\/jats:sub>\n                    .\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0246375","type":"journal-article","created":{"date-parts":[[2021,2,2]],"date-time":"2021-02-02T14:23:13Z","timestamp":1612275793000},"page":"e0246375","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":7,"title":["Cross-effect of TRPV1 and EP3 receptor on coughs and bronchopulmonary C-neural activities"],"prefix":"10.1371","volume":"16","author":[{"given":"Xiuping","family":"Gao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4579-046X","authenticated-orcid":true,"given":"Jianguo","family":"Zhuang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wan","family":"Wei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fadi","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2021,2,2]]},"reference":[{"issue":"3","key":"pone.0246375.ref001","doi-asserted-by":"crossref","first-page":"1007","DOI":"10.1152\/jappl.1988.65.3.1007","article-title":"Afferent neural pathways in cough and reflex bronchoconstriction","volume":"65","author":"JA Karlsson","year":"1988","journal-title":"Journal of applied physiology"},{"key":"pone.0246375.ref002","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1007\/BF02713631","article-title":"Cough: physiology, evaluation, and treatment","volume":"164","author":"AS Banner","year":"1986","journal-title":"Lung"},{"issue":"159","key":"pone.0246375.ref003","first-page":"1","article-title":"Ambulatory care visits to physician offices, hospital outpatient departments, and emergency departments: United States, 2001\u201302","author":"SM Schappert","year":"2006","journal-title":"Vital and health statistics Series 13, Data from the National Health Survey"},{"issue":"3","key":"pone.0246375.ref004","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1111\/j.1742-1241.1998.tb11595.x","article-title":"A retrospective survey of diagnosis and management of patients presenting with chronic cough to a general chest clinic","volume":"52","author":"LP McGarvey","year":"1998","journal-title":"International journal of clinical practice"},{"issue":"11","key":"pone.0246375.ref005","doi-asserted-by":"crossref","first-page":"975","DOI":"10.1136\/thx.2006.060087","article-title":"Cough in the community: a cross sectional survey and the relationship to gastrointestinal symptoms","volume":"61","author":"AC Ford","year":"2006","journal-title":"Thorax"},{"issue":"187","key":"pone.0246375.ref006","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1007\/978-3-540-79842-2_2","article-title":"Cough sensors. I. Physiological and pharmacological properties of the afferent nerves regulating cough","author":"BJ Canning","year":"2009","journal-title":"Handbook of experimental pharmacology"},{"issue":"2","key":"pone.0246375.ref007","doi-asserted-by":"crossref","first-page":"R369","DOI":"10.1152\/ajpregu.00044.2010","article-title":"Encoding of the cough reflex in anesthetized guinea pigs","volume":"300","author":"BJ Canning","year":"2011","journal-title":"Am J Physiol Regul Integr Comp Physiol"},{"issue":"6","key":"pone.0246375.ref008","doi-asserted-by":"crossref","first-page":"1943","DOI":"10.1164\/ajrccm.159.6.9808078","article-title":"Identification and substance P content of vagal afferent neurons innervating the epithelium of the guinea pig trachea","volume":"159","author":"DD Hunter","year":"1999","journal-title":"Am J Respir Crit Care Med"},{"issue":"5585","key":"pone.0246375.ref009","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1038\/264451a0","article-title":"Stimulation of \u2019irritant\u2019 receptors and afferent C-fibres in the lungs by prostaglandins","volume":"264","author":"HM Coleridge","year":"1976","journal-title":"Nature"},{"issue":"2","key":"pone.0246375.ref010","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1111\/j.1748-1716.1986.tb07981.x","article-title":"Cough induced by stimulation of capsaicin-sensitive sensory neurons in conscious guinea-pigs","volume":"128","author":"K Forsberg","year":"1986","journal-title":"Acta physiologica Scandinavica"},{"issue":"5","key":"pone.0246375.ref011","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1016\/j.pupt.2013.03.010","article-title":"Acid-sensing by airway afferent nerves","volume":"26","author":"LY Lee","year":"2013","journal-title":"Pulmonary pharmacology & therapeutics"},{"issue":"5905","key":"pone.0246375.ref012","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1038\/302251a0","article-title":"Capsaicin-induced desensitization of airway mucosa to cigarette smoke, mechanical and chemical irritants","volume":"302","author":"JM Lundberg","year":"1983","journal-title":"Nature"},{"issue":"1","key":"pone.0246375.ref013","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/0952-0600(88)90008-7","article-title":"Cough and bronchoconstriction mediated by capsaicin-sensitive sensory neurons in the guinea-pig","volume":"1","author":"K Forsberg","year":"1988","journal-title":"Pulmonary pharmacology"},{"issue":"10","key":"pone.0246375.ref014","doi-asserted-by":"crossref","first-page":"923","DOI":"10.1164\/rccm.200903-0388OC","article-title":"Prostaglandin E2 mediates cough via the EP3 receptor: implications for future disease therapy","volume":"180","author":"SA Maher","year":"2009","journal-title":"Am J Respir Crit Care Med"},{"issue":"Suppl 1","key":"pone.0246375.ref015","doi-asserted-by":"crossref","first-page":"S9","DOI":"10.1007\/s00408-009-9190-2","article-title":"Prostanoids and the cough reflex","volume":"188","author":"SA Maher","year":"2010","journal-title":"Lung"},{"issue":"1","key":"pone.0246375.ref016","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1111\/j.1476-5381.1991.tb12383.x","article-title":"Prostacyclin activates tachykinin release from capsaicin-sensitive afferents in guinea-pig bronchi through a ruthenium red-sensitive pathway","volume":"104","author":"CE Mapp","year":"1991","journal-title":"British journal of pharmacology"},{"key":"pone.0246375.ref017","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1186\/1745-9974-3-2","article-title":"Prostaglandin I2 enhances cough reflex sensitivity to capsaicin in the asthmatic airway","volume":"3","author":"Y Ishiura","year":"2007","journal-title":"Cough"},{"issue":"6653","key":"pone.0246375.ref018","doi-asserted-by":"crossref","first-page":"816","DOI":"10.1038\/39807","article-title":"The capsaicin receptor: a heat-activated ion channel in the pain pathway","volume":"389","author":"MJ Caterina","year":"1997","journal-title":"Nature"},{"issue":"2","key":"pone.0246375.ref019","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1038\/sj.bjp.0703050","article-title":"The endogenous lipid anandamide is a full agonist at the human vanilloid receptor (hVR1)","volume":"129","author":"D Smart","year":"2000","journal-title":"British journal of pharmacology"},{"issue":"1","key":"pone.0246375.ref020","doi-asserted-by":"crossref","first-page":"L58","DOI":"10.1152\/ajplung.00517.2005","article-title":"Characterization of acid signaling in rat vagal pulmonary sensory neurons","volume":"291","author":"Q Gu","year":"2006","journal-title":"American journal of physiology Lung cellular and molecular physiology"},{"issue":"9","key":"pone.0246375.ref021","doi-asserted-by":"crossref","first-page":"644","DOI":"10.1007\/s13238-017-0395-5","article-title":"TRPV1 and TRPA1 in cutaneous neurogenic and chronic inflammation: pro-inflammatory response induced by their activation and their sensitization","volume":"8","author":"O Gouin","year":"2017","journal-title":"Protein & cell"},{"issue":"8","key":"pone.0246375.ref022","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1038\/nrd3456","article-title":"Transient receptor potential channels as therapeutic targets","volume":"10","author":"MM Moran","year":"2011","journal-title":"Nature reviews Drug discovery"},{"issue":"16","key":"pone.0246375.ref023","doi-asserted-by":"crossref","first-page":"11613","DOI":"10.1074\/jbc.R600038200","article-title":"Prostaglandin E receptors","volume":"282","author":"Y Sugimoto","year":"2007","journal-title":"The Journal of biological chemistry"},{"issue":"3","key":"pone.0246375.ref024","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/j.coph.2011.06.005","article-title":"G-protein coupled receptors regulating cough","volume":"11","author":"SA Maher","year":"2011","journal-title":"Current opinion in pharmacology"},{"issue":"4","key":"pone.0246375.ref025","doi-asserted-by":"crossref","first-page":"1193","DOI":"10.1152\/physrev.1999.79.4.1193","article-title":"Prostanoid receptors: structures, properties, and functions","volume":"79","author":"S Narumiya","year":"1999","journal-title":"Physiological reviews"},{"issue":"10","key":"pone.0246375.ref026","doi-asserted-by":"crossref","first-page":"891","DOI":"10.1136\/thoraxjnl-2011-201443","article-title":"Transient receptor potential channels mediate the tussive response to prostaglandin E2 and bradykinin","volume":"67","author":"M Grace","year":"2012","journal-title":"Thorax"},{"issue":"1","key":"pone.0246375.ref027","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1164\/ajrccm\/140.1.137","article-title":"Sensitivity of the human cough reflex: effect of inflammatory mediators prostaglandin E2, bradykinin, and histamine","volume":"140","author":"NB Choudry","year":"1989","journal-title":"The American review of respiratory disease"},{"key":"pone.0246375.ref028","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.pupt.2017.06.009","article-title":"Cough and airway disease: The role of ion channels","volume":"47","author":"SJ Bonvini","year":"2017","journal-title":"Pulmonary pharmacology & therapeutics"},{"issue":"4","key":"pone.0246375.ref029","doi-asserted-by":"crossref","first-page":"1985","DOI":"10.1152\/jn.00748.2002","article-title":"Ca2+ transient evoked by chemical stimulation is enhanced by PGE2 in vagal sensory neurons: role of cAMP\/PKA signaling pathway","volume":"89","author":"Q Gu","year":"2003","journal-title":"Journal of neurophysiology"},{"issue":"4","key":"pone.0246375.ref030","doi-asserted-by":"crossref","first-page":"1419","DOI":"10.1152\/japplphysiol.00382.2002","article-title":"PGE(2) sensitizes cultured pulmonary vagal sensory neurons to chemical and electrical stimuli","volume":"93","author":"K Kwong","year":"2002","journal-title":"Journal of applied physiology"},{"issue":"2","key":"pone.0246375.ref031","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1111\/j.1471-4159.1991.tb08182.x","article-title":"Characterization of prostaglandin E2-induced Ca2+ mobilization in single bovine adrenal chromaffin cells by digital image microscopy","volume":"56","author":"S Ito","year":"1991","journal-title":"Journal of neurochemistry"},{"issue":"48","key":"pone.0246375.ref032","doi-asserted-by":"crossref","first-page":"14496","DOI":"10.1021\/bi00252a016","article-title":"Isoforms of the EP3 subtype of human prostaglandin E2 receptor transduce both intracellular calcium and cAMP signals","volume":"33","author":"S An","year":"1994","journal-title":"Biochemistry"},{"issue":"5","key":"pone.0246375.ref033","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1016\/j.ceca.2013.08.005","article-title":"Lack of correlation between the amplitudes of TRP channel-mediated responses to weak and strong stimuli in intracellular Ca(2+) imaging experiments","volume":"54","author":"YA Alpizar","year":"2013","journal-title":"Cell calcium"},{"issue":"9","key":"pone.0246375.ref034","doi-asserted-by":"crossref","first-page":"L922","DOI":"10.1152\/ajplung.00241.2014","article-title":"Prenatal nicotinic exposure augments cardiorespiratory responses to activation of bronchopulmonary C-fibers","volume":"308","author":"J Zhuang","year":"2015","journal-title":"American journal of physiology Lung cellular and molecular physiology"},{"key":"pone.0246375.ref035","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.taap.2015.10.023","article-title":"Prenatal nicotinic exposure upregulates pulmonary C-fiber NK1R expression to prolong pulmonary C-fiber-mediated apneic response","volume":"290","author":"L Zhao","year":"2016","journal-title":"Toxicology and applied pharmacology"},{"issue":"4","key":"pone.0246375.ref036","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1038\/sj.bjp.0706212","article-title":"Prostaglandin E2-induced modification of tetrodotoxin-resistant Na+ currents involves activation of both EP2 and EP4 receptors in neonatal rat nodose ganglion neurones","volume":"145","author":"S Matsumoto","year":"2005","journal-title":"British journal of pharmacology"},{"issue":"33","key":"pone.0246375.ref037","doi-asserted-by":"crossref","first-page":"13648","DOI":"10.1073\/pnas.1300820110","article-title":"Neuronal prostaglandin E2 receptor subtype EP3 mediates antinociception during inflammation","volume":"110","author":"G Natura","year":"2013","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"issue":"10","key":"pone.0246375.ref038","doi-asserted-by":"crossref","first-page":"4325","DOI":"10.1096\/fj.201700163R","article-title":"Prenatal nicotinic exposure prolongs superior laryngeal C-fiber-mediated apnea and bradycardia through enhancing neuronal TRPV1 expression and excitation","volume":"31","author":"X Gao","year":"2017","journal-title":"FASEB journal: official publication of the Federation of American Societies for Experimental Biology"},{"issue":"5","key":"pone.0246375.ref039","doi-asserted-by":"crossref","first-page":"e0217366","DOI":"10.1371\/journal.pone.0217366","article-title":"An advanced recording and analysis system for the differentiation of guinea pig cough responses to citric acid and prostaglandin E2 in real time","volume":"14","author":"J Zhuang","year":"2019","journal-title":"PloS one"},{"issue":"2","key":"pone.0246375.ref040","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1124\/jpet.107.127258","article-title":"Pharmacology and antitussive efficacy of 4-(3-trifluoromethyl-pyridin-2-yl)-piperazine-1-carboxylic acid (5-trifluoromethyl-pyridin-2-yl)-amide (JNJ17203212), a transient receptor potential vanilloid 1 antagonist in guinea pigs","volume":"323","author":"A Bhattacharya","year":"2007","journal-title":"The Journal of pharmacology and experimental therapeutics"},{"key":"pone.0246375.ref041","first-page":"fj201900279RR","article-title":"Prolongation of bronchopulmonary C-fiber-mediated apnea by prenatal nicotinic exposure in rat pups: role of 5-HT3 receptors","author":"L Zhao","year":"2019","journal-title":"FASEB journal: official publication of the Federation of American Societies for Experimental Biology"},{"issue":"1","key":"pone.0246375.ref042","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/j.bbrc.2014.11.053","article-title":"Prostaglandin potentiates 5-HT responses in stomach and ileum innervating visceral afferent sensory neurons","volume":"456","author":"S Kim","year":"2015","journal-title":"Biochemical and biophysical research communications"},{"issue":"4","key":"pone.0246375.ref043","doi-asserted-by":"crossref","first-page":"863","DOI":"10.1111\/j.1476-5381.2010.01087.x","article-title":"Roles of affinity and lipophilicity in the slow kinetics of prostanoid receptor antagonists on isolated smooth muscle preparations","volume":"162","author":"RL Jones","year":"2011","journal-title":"British journal of pharmacology"},{"issue":"3","key":"pone.0246375.ref044","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1124\/jpet.117.242099","article-title":"Preclinical Pharmacology and Pharmacokinetics of Inhaled Hexadecyl-Treprostinil (C16TR), a Pulmonary Vasodilator Prodrug","volume":"363","author":"MR Corboz","year":"2017","journal-title":"The Journal of pharmacology and experimental therapeutics"},{"issue":"187","key":"pone.0246375.ref045","first-page":"1","article-title":"Cough: setting the scene","author":"KF Chung","year":"2009","journal-title":"Handbook of experimental pharmacology"},{"issue":"4","key":"pone.0246375.ref046","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1111\/j.1365-2125.1985.tb05077.x","article-title":"Characteristics of prostaglandin induced cough in man","volume":"20","author":"JF Costello","year":"1985","journal-title":"Br J Clin Pharmacol"},{"issue":"1","key":"pone.0246375.ref047","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1164\/ajrccm.159.1.9804030","article-title":"Protective effects of inhaled PGE2 on allergen-induced airway responses and airway inflammation","volume":"159","author":"GM Gauvreau","year":"1999","journal-title":"Am J Respir Crit Care Med"},{"issue":"2","key":"pone.0246375.ref048","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1007\/BF00562439","article-title":"Evaluation of aerosols of prostaglandins E1 and E2 as bronchodilators","volume":"6","author":"Y Kawakami","year":"1973","journal-title":"European journal of clinical pharmacology"},{"key":"pone.0246375.ref049","first-page":"A667","article-title":"Distinct patterns of response to tussive agents in a guinea-pig model of cough: effect of cigarette smoke exposure","volume":"41","author":"J BM Nasra","year":"2007","journal-title":"Am J Respir Crit Care Med"},{"issue":"1","key":"pone.0246375.ref050","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1254\/jphs.FPJ05014X","article-title":"Mechanisms of capsaicin- and citric-acid-induced cough reflexes in guinea pigs","volume":"99","author":"M Tanaka","year":"2005","journal-title":"Journal of pharmacological sciences"},{"issue":"Suppl 3","key":"pone.0246375.ref051","doi-asserted-by":"crossref","first-page":"S59","DOI":"10.33549\/physiolres.931478","article-title":"Calcium-dependent desensitization of vanilloid receptor TRPV1: a mechanism possibly involved in analgesia induced by topical application of capsaicin","volume":"57","author":"L Vyklicky","year":"2008","journal-title":"Physiological research"},{"issue":"1","key":"pone.0246375.ref052","doi-asserted-by":"crossref","first-page":"122","DOI":"10.2174\/138920111793937826","article-title":"A \"cute\" desensitization of TRPV1","volume":"12","author":"F Touska","year":"2011","journal-title":"Current pharmaceutical biotechnology"},{"key":"pone.0246375.ref053","doi-asserted-by":"crossref","first-page":"5806321","DOI":"10.1155\/2019\/5806321","article-title":"Multifunctional TRPV1 Ion Channels in Physiology and Pathology with Focus on the Brain, Vasculature, and Some Visceral Systems","volume":"2019","author":"MV Storozhuk","year":"2019","journal-title":"BioMed research international"},{"issue":"4\u20135","key":"pone.0246375.ref054","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1002\/mnfr.200500207","article-title":"The differential role of prostaglandin E2 receptors EP3 and EP4 in regulation of fever","volume":"50","author":"M Lazarus","year":"2006","journal-title":"Molecular nutrition & food research"},{"issue":"6","key":"pone.0246375.ref055","doi-asserted-by":"crossref","first-page":"F1269","DOI":"10.1152\/ajprenal.00230.2004","article-title":"Activation of EP4 receptors contributes to prostaglandin E2-mediated stimulation of renal sensory nerves","volume":"287","author":"UC Kopp","year":"2004","journal-title":"American journal of physiology Renal physiology"},{"issue":"22","key":"pone.0246375.ref056","doi-asserted-by":"crossref","first-page":"9471","DOI":"10.1523\/JNEUROSCI.18-22-09471.1998","article-title":"Activation of vagal afferents after intravenous injection of interleukin-1beta: role of endogenous prostaglandins","volume":"18","author":"M Ek","year":"1998","journal-title":"The Journal of neuroscience: the official journal of the Society for Neuroscience"},{"issue":"3","key":"pone.0246375.ref057","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1016\/j.resp.2006.11.003","article-title":"Prostaglandin E2 enhances the sensitizing effect of hyperthermia on pulmonary C-fibers in rats","volume":"156","author":"G Zhang","year":"2007","journal-title":"Respiratory physiology & neurobiology"},{"issue":"6","key":"pone.0246375.ref058","doi-asserted-by":"crossref","first-page":"1918","DOI":"10.1152\/japplphysiol.01276.2005","article-title":"Prostaglandin involvement in lung C-fiber activation by substance P in guinea pigs","volume":"100","author":"DR Bergren","year":"2006","journal-title":"Journal of applied physiology"},{"key":"pone.0246375.ref059","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.pupt.2015.08.003","article-title":"Interaction between TRPA1 and TRPV1: Synergy on pulmonary sensory nerves","volume":"35","author":"LY Lee","year":"2015","journal-title":"Pulmonary pharmacology & therapeutics"},{"key":"pone.0246375.ref060","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1113\/jphysiol.1993.sp019804","article-title":"Effects of aerosol-applied capsaicin, histamine and prostaglandin E2 on airway sensory receptors of anaesthetized cats","volume":"469","author":"SP Mohammed","year":"1993","journal-title":"J Physiol"},{"issue":"2 Pt 1","key":"pone.0246375.ref061","first-page":"528","article-title":"Prostaglandin E(2) enhances chemical and mechanical sensitivities of pulmonary C fibers in the rat","volume":"162","author":"CY Ho","year":"2000","journal-title":"Am J Respir Crit Care Med"},{"issue":"5","key":"pone.0246375.ref062","doi-asserted-by":"crossref","first-page":"1679","DOI":"10.1152\/jappl.1995.79.5.1679","article-title":"Pulmonary chemoreflex sensitivity is enhanced by prostaglandin E2 in anesthetized rats","volume":"79","author":"LY Lee","year":"1995","journal-title":"Journal of applied physiology"},{"issue":"2\u20133","key":"pone.0246375.ref063","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.lfs.2003.09.002","article-title":"Functional domains essential for Gs activity in prostaglandin EP2 and EP3 receptors","volume":"74","author":"Y Sugimoto","year":"2003","journal-title":"Life sciences"},{"issue":"6","key":"pone.0246375.ref064","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1093\/oxfordjournals.jbchem.a003165","article-title":"Prostaglandin receptors: advances in the study of EP3 receptor signaling","volume":"131","author":"N Hatae","year":"2002","journal-title":"Journal of biochemistry"},{"key":"pone.0246375.ref065","article-title":"Diacylglycerol kinases regulate TRPV1 channel activity","author":"L Liu","year":"2020","journal-title":"The Journal of biological chemistry"},{"issue":"1","key":"pone.0246375.ref066","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/0306-4522(91)90255-M","article-title":"Further confirmation of the role of adenyl cyclase and of cAMP-dependent protein kinase in primary afferent hyperalgesia","volume":"44","author":"YO Taiwo","year":"1991","journal-title":"Neuroscience"},{"issue":"3","key":"pone.0246375.ref067","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1016\/0306-4522(89)90280-7","article-title":"Mediation of primary afferent peripheral hyperalgesia by the cAMP second messenger system","volume":"32","author":"YO Taiwo","year":"1989","journal-title":"Neuroscience"},{"issue":"4","key":"pone.0246375.ref068","article-title":"Role of Transient Receptor Potential Channels Trpv1 and Trpm8 in Diabetic Peripheral Neuropathy","volume":"2017","author":"MR Pabbidi","year":"2017","journal-title":"J Diabetes Treat"},{"issue":"17","key":"pone.0246375.ref069","doi-asserted-by":"crossref","first-page":"7444","DOI":"10.1523\/JNEUROSCI.22-17-07444.2002","article-title":"Prostaglandin and protein kinase A-dependent modulation of vanilloid receptor function by metabotropic glutamate receptor 5: potential mechanism for thermal hyperalgesia","volume":"22","author":"HJ Hu","year":"2002","journal-title":"The Journal of neuroscience: the official journal of the Society for Neuroscience"},{"issue":"16","key":"pone.0246375.ref070","doi-asserted-by":"crossref","first-page":"6081","DOI":"10.1523\/JNEUROSCI.18-16-06081.1998","article-title":"The cAMP transduction cascade mediates the prostaglandin E2 enhancement of the capsaicin-elicited current in rat sensory neurons: whole-cell and single-channel studies","volume":"18","author":"JC Lopshire","year":"1998","journal-title":"The Journal of neuroscience: the official journal of the Society for Neuroscience"},{"issue":"50","key":"pone.0246375.ref071","doi-asserted-by":"crossref","first-page":"50080","DOI":"10.1074\/jbc.M306619200","article-title":"Desensitization of capsaicin-activated currents in the vanilloid receptor TRPV1 is decreased by the cyclic AMP-dependent protein kinase pathway","volume":"278","author":"DP Mohapatra","year":"2003","journal-title":"The Journal of biological chemistry"},{"issue":"23","key":"pone.0246375.ref072","doi-asserted-by":"crossref","first-page":"19462","DOI":"10.1074\/jbc.M111.289751","article-title":"Agonist- and Ca2+-dependent desensitization of TRPV1 channel targets the receptor to lysosomes for degradation","volume":"287","author":"L Sanz-Salvador","year":"2012","journal-title":"The Journal of biological chemistry"},{"issue":"2","key":"pone.0246375.ref073","doi-asserted-by":"crossref","first-page":"466","DOI":"10.1164\/ajrccm\/147.2.466","article-title":"Airway and pulmonary tissue responses to capsaicin in guinea pigs assessed with the alveolar capsule technique","volume":"147","author":"MA Martins","year":"1993","journal-title":"The American review of respiratory disease"},{"issue":"2","key":"pone.0246375.ref074","doi-asserted-by":"crossref","first-page":"798","DOI":"10.1152\/jappl.1993.75.2.798","article-title":"Effects of capsaicin desensitization on nasal mucosal secretion in guinea pigs in vivo","volume":"75","author":"AZ Gawin","year":"1993","journal-title":"Journal of applied physiology"},{"issue":"1","key":"pone.0246375.ref075","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1164\/rccm.200308-1092OC","article-title":"Induced sputum inflammatory mediator concentrations in chronic cough","volume":"169","author":"SS Birring","year":"2004","journal-title":"Am J Respir Crit Care Med"},{"issue":"4","key":"pone.0246375.ref076","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1016\/S0091-6749(03)01889-X","article-title":"Increased prostaglandin E2 concentrations and cyclooxygenase-2 expression in asthmatic subjects with sputum eosinophilia","volume":"112","author":"M Profita","year":"2003","journal-title":"The Journal of allergy and clinical immunology"},{"issue":"1","key":"pone.0246375.ref077","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1164\/ajrccm\/143.1.188","article-title":"Arachidonic acid metabolism. Implications of biological chemistry for lung function and disease","volume":"143","author":"MJ Holtzman","year":"1991","journal-title":"The American review of respiratory disease"},{"issue":"3","key":"pone.0246375.ref078","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1183\/09031936.95.08030416","article-title":"The effect of 1 ppm nitrogen dioxide on bronchoalveolar lavage cells and inflammatory mediators in normal and asthmatic subjects","volume":"8","author":"R Jorres","year":"1995","journal-title":"Eur Respir J"},{"issue":"1","key":"pone.0246375.ref079","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/0090-6980(88)90275-4","article-title":"Profiling of bisenoic prostaglandins and thromboxane B2 in bronchoalveolar fluid from the lower respiratory tract of human subjects by combined capillary gas chromatography-mass spectrometry","volume":"35","author":"MC Liu","year":"1988","journal-title":"Prostaglandins"},{"issue":"1\u20133","key":"pone.0246375.ref080","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/j.ejphar.2005.12.063","article-title":"The transient receptor potential vanilloid 1: role in airway inflammation and disease","volume":"533","author":"P Geppetti","year":"2006","journal-title":"European journal of pharmacology"},{"issue":"2","key":"pone.0246375.ref081","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1152\/jappl.1998.84.2.417","article-title":"Role of pulmonary C fibers in adenosine-induced respiratory inhibition in anesthetized rats","volume":"84","author":"K Kwong","year":"1998","journal-title":"Journal of applied physiology"},{"issue":"4","key":"pone.0246375.ref082","doi-asserted-by":"crossref","first-page":"1446","DOI":"10.1152\/japplphysiol.00767.2002","article-title":"Acute hypoxia prolongs the apnea induced by right atrial injection of capsaicin","volume":"94","author":"F Xu","year":"2003","journal-title":"Journal of applied physiology"},{"issue":"12","key":"pone.0246375.ref083","doi-asserted-by":"crossref","first-page":"1276","DOI":"10.1164\/rccm.200402-174OC","article-title":"Increased expression of transient receptor potential vanilloid-1 in airway nerves of chronic cough","volume":"170","author":"DA Groneberg","year":"2004","journal-title":"Am J Respir Crit Care Med"},{"issue":"Suppl 1","key":"pone.0246375.ref084","doi-asserted-by":"crossref","first-page":"S88","DOI":"10.1007\/s00408-007-9068-0","article-title":"Irritant-induced chronic cough: irritant-induced TRPpathy","volume":"186","author":"SM Brooks","year":"2008","journal-title":"Lung"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0246375","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,19]],"date-time":"2023-10-19T21:45:09Z","timestamp":1697751909000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0246375"}},"subtitle":[],"editor":[{"given":"Alexander G","family":"Obukhov","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2021,2,2]]},"references-count":84,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2021,2,2]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0246375","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,2,2]]}}}