{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T14:07:52Z","timestamp":1740146872350,"version":"3.37.3"},"reference-count":59,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2021,2,11]],"date-time":"2021-02-11T00:00:00Z","timestamp":1613001600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"funder":[{"DOI":"10.13039\/100000038","name":"U.S. Food and Drug Administration","doi-asserted-by":"publisher","award":["CPOSEL13"],"award-info":[{"award-number":["CPOSEL13"]}],"id":[{"id":"10.13039\/100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000185","name":"Defense Advanced Research Projects Agency","doi-asserted-by":"publisher","award":["DARPA-FDA IAA No. 224-14-6009"],"award-info":[{"award-number":["DARPA-FDA IAA No. 224-14-6009"]}],"id":[{"id":"10.13039\/100000185","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>To evaluate movement quality of upper limb (UL) prosthesis users, performance-based outcome measures have been developed that examine the normalcy of movement as compared to a person with a sound, intact hand. However, the broad definition of \u201cnormal movement\u201d and the subjective nature of scoring can make it difficult to know which areas of the body to evaluate, and the expected magnitude of deviation from normative movement. To provide a more robust approach to characterizing movement differences, the goals of this work are to identify degrees of freedom (DOFs) that will inform abnormal movement for several tasks using unsupervised machine learning (clustering methods) and elucidate the variations in movement approach across two upper-limb prosthesis devices with varying DOFs as compared to healthy controls. 24 participants with no UL disability or impairment were recruited for this study and trained on the use of a body-powered bypass (n = 6) or the DEKA limb bypass (n = 6) prosthetic devices or included as normative controls. 3D motion capture data were collected from all participants as they performed the Jebsen-Taylor Hand Function Test (JHFT) and targeted Box and Blocks Test (tBBT). Range of Motion, peak angle, angular path length, mean angle, peak angular velocity, and number of zero crossings were calculated from joint angle data for the right\/left elbows, right\/left shoulders, torso, and neck and fed into a K-means clustering algorithm. Results show right shoulder and torso DOFs to be most informative in distinguishing between bypass user and norm group movement. The JHFT page turning task and the seated tBBT elicit movements from bypass users that are most distinctive from the norm group. Results can be used to inform the development of movement quality scoring methodology for UL performance-based outcome measures. Identifying tasks across two different devices with known variations in movement can inform the best tasks to perform in a rehabilitation setting that challenge the prosthesis user\u2019s ability to achieve normative movement.<\/jats:p>","DOI":"10.1371\/journal.pone.0246795","type":"journal-article","created":{"date-parts":[[2021,2,12]],"date-time":"2021-02-12T18:39:12Z","timestamp":1613155152000},"page":"e0246795","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":3,"title":["Application of machine learning to the identification of joint degrees of freedom involved in abnormal movement during upper limb prosthesis use"],"prefix":"10.1371","volume":"16","author":[{"given":"Sophie 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and kinematics","volume":"13","author":"LH Huinink","year":"2016","journal-title":"J Neuroeng Rehabil"},{"issue":"1","key":"pone.0246795.ref031","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1097\/01241398-199301000-00014","article-title":"Myoelectric and body-powered prostheses","volume":"13","author":"LM Kruger","year":"1993","journal-title":"J Pediatr Orthop"},{"issue":"1","key":"pone.0246795.ref032","doi-asserted-by":"crossref","first-page":"27","DOI":"10.3109\/03093648609103076","article-title":"Prosthetic use in adult upper limb amputees: a comparison of the body powered and electrically powered prostheses","volume":"10","author":"SG Millstein","year":"1986","journal-title":"Prosthet Orthot Int"},{"issue":"1","key":"pone.0246795.ref033","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1682\/JRRD.2014.03.0087","article-title":"Design and evaluation of voluntary opening and voluntary closing prosthetic terminal device","volume":"52","author":"JW Sensinger","year":"2015","journal-title":"J Rehabil Res Dev"},{"issue":"1","key":"pone.0246795.ref034","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1177\/0309364616631348","article-title":"Movement quality of conventional prostheses and the DEKA Arm during everyday tasks","volume":"41","author":"J Cowley","year":"2017","journal-title":"Prosthet Orthot Int"},{"key":"pone.0246795.ref035","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1186\/1743-0003-11-16","article-title":"Changes in performance over time while learning to use a myoelectric prosthesis","volume":"11","author":"H Bouwsema","year":"2014","journal-title":"J Neuroeng Rehabil"},{"issue":"2","key":"pone.0246795.ref036","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1682\/JRRD.2013.05.0123","article-title":"Comparison of body-powered voluntary opening and voluntary closing prehensor for activities of daily life","volume":"51","author":"K Berning","year":"2014","journal-title":"J Rehabil Res Dev"},{"issue":"3","key":"pone.0246795.ref037","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1053\/apmr.2003.50014","article-title":"Training with an upper-limb prosthetic simulator to enhance transfer of skill across limbs","volume":"84","author":"DL Weeks","year":"2003","journal-title":"Archives of physical medicine and rehabilitation"},{"issue":"6","key":"pone.0246795.ref038","doi-asserted-by":"crossref","first-page":"1","DOI":"10.15761\/PMRR.1000191","article-title":"Creating a standardized, quantitative training protocol for upper limb bypass prostheses","volume":"3","author":"C Bloomer","year":"2018","journal-title":"Phys Med Rehabil Res"},{"issue":"1","key":"pone.0246795.ref039","doi-asserted-by":"crossref","first-page":"e0226563","DOI":"10.1371\/journal.pone.0226563","article-title":"Kinematic analysis of motor learning in upper limb body-powered bypass prosthesis training","volume":"15","author":"C Bloomer","year":"2020","journal-title":"PLoS One"},{"key":"pone.0246795.ref040","unstructured":"Atkins D. Adult Upper Limb Prosthetic Training. Atlas of Limb Prosthetics: Surgical, Prosthetic, and Rehabilitation Principles. 2 ed. Rosemont, IL: American Academy of Orthopedic Surgeons; 1992."},{"issue":"3","key":"pone.0246795.ref041","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/S0268-0890(97)90034-7","article-title":"Prosthetic management and training of adult upper limb amputees","volume":"11","author":"R Dakpa","year":"1997","journal-title":"Current Orthopaedics"},{"key":"pone.0246795.ref042","article-title":"Assessing kinematic variability during performance of Jebsen-Taylor Hand Function Test","author":"KL Kontson","year":"2019","journal-title":"J Hand Ther"},{"issue":"5","key":"pone.0246795.ref043","doi-asserted-by":"crossref","first-page":"e0177965","DOI":"10.1371\/journal.pone.0177965","article-title":"Targeted box and blocks test: Normative data and comparison to standard tests","volume":"12","author":"K Kontson","year":"2017","journal-title":"PLoS One"},{"issue":"3","key":"pone.0246795.ref044","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1016\/j.gaitpost.2004.04.001","article-title":"Soft tissue artefact assessment in humeral axial rotation","volume":"21","author":"AG Cutti","year":"2005","journal-title":"Gait & Posture"},{"key":"pone.0246795.ref045","unstructured":"Muray IA. Determining Upper Limb Kinematics and Dynamics During Everyday Tasks: University of Newcastle; 1999."},{"key":"pone.0246795.ref046","unstructured":"Stagni R. FS, Cappello A., Ussia L., Leardini A. Third Siamoc Congress: Propagation of skin motion artefacts to knee joint kinematics. Gait & Posture. 2002;16:211\u20132."},{"key":"pone.0246795.ref047","article-title":"Evaluation of Performance-Based Outcome Measures for the Upper Limb: A Systematic Review","author":"S Wang","year":"2018","journal-title":"PM & R: the journal of injury, function, and rehabilitation"},{"issue":"1","key":"pone.0246795.ref048","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1186\/1475-925X-13-20","article-title":"Kinematic evaluation of movement smoothness in golf: relationship between the normalized jerk cost of body joints and the clubhead","volume":"13","author":"A Choi","year":"2014","journal-title":"Biomedical engineering online"},{"issue":"3","key":"pone.0246795.ref049","article-title":"Kinematic analysis of the upper extremity after stroke\u2014how far have we reached and what have we grasped?","volume":"20","author":"MAH Murphy","year":"2015","journal-title":"Physical Therapy Reviews"},{"issue":"4","key":"pone.0246795.ref050","doi-asserted-by":"crossref","first-page":"1062","DOI":"10.1016\/j.gaitpost.2014.01.008","article-title":"Accuracy of the Microsoft Kinect sensor for measuring movement in people with Parkinson\u2019s disease","volume":"39","author":"B Galna","year":"2014","journal-title":"Gait Posture"},{"key":"pone.0246795.ref051","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1186\/s12891-016-0957-3","article-title":"Validity and reliability of arm abduction angle measured on smartphone: a cross-sectional study","volume":"17","author":"AI Cuesta-Vargas","year":"2016","journal-title":"BMC Musculoskelet Disord"},{"issue":"5","key":"pone.0246795.ref052","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1016\/j.otsr.2009.04.012","article-title":"Upper extremity kinematics analysis in obstetrical brachial plexus palsy","volume":"95","author":"F Fitoussi","year":"2009","journal-title":"Orthop Traumatol Surg Res"},{"key":"pone.0246795.ref053","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1016\/j.gaitpost.2019.01.037","article-title":"Characterization of normative angular joint kinematics during two functional upper limb tasks","volume":"69","author":"AM Valevicius","year":"2019","journal-title":"Gait Posture"},{"issue":"1","key":"pone.0246795.ref054","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/j.gaitpost.2007.03.002","article-title":"Complete 3D kinematics of upper extremity functional tasks","volume":"27","author":"CJ van Andel","year":"2008","journal-title":"Gait Posture"},{"issue":"10","key":"pone.0246795.ref055","doi-asserted-by":"crossref","first-page":"1874","DOI":"10.1109\/TNSRE.2017.2693234","article-title":"An Integrated Movement Analysis Framework to Study Upper Limb Function: A Pilot Study","volume":"25","author":"KL Kontson","year":"2017","journal-title":"IEEE Trans Neural Syst Rehabil 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