{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T03:01:20Z","timestamp":1787022080848,"version":"build-2736575974"},"reference-count":55,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2022,2,10]],"date-time":"2022-02-10T00:00:00Z","timestamp":1644451200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100012895","name":"Zoetis","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100012895","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>\n                    A cross-sectional prospective cohort study including 1026 heifers administered tulathromycin due to high risk of clinical signs of bovine respiratory disease (BRD), measured poor association between BRD clinical outcomes and results of bacterial culture and tulathromycin susceptibility from BRD isolates of deep nasopharyngeal swabs (DNS) and adequate association with viral polymerase chain reaction (PCR) results from nasal swabs. Isolation rates from DNS collected on day-0 and at 1\n                    <jats:sup>st<\/jats:sup>\n                    BRD-treatment respectively were:\n                    <jats:italic>Mannheimia haemolytica<\/jats:italic>\n                    (10.9% &amp; 34.1%);\n                    <jats:italic>Pasteurella multocida<\/jats:italic>\n                    (10.4% &amp; 7.4%);\n                    <jats:italic>Mycoplasma bovis<\/jats:italic>\n                    (1.0% &amp; 36.6%); and\n                    <jats:italic>Histophilus somni<\/jats:italic>\n                    (0.7% &amp; 6.3%). Prevalence of BRD viral nucleic acid on nasal swabs collected exclusively at 1\n                    <jats:sup>st<\/jats:sup>\n                    BRD-treatment were: bovine parainfluenza virus type-3 (bPIV-3) 34.1%; bovine viral diarrhea virus (BVDV) 26.3%; bovine herpes virus type-1 (BHV-1) 10.8%; and bovine respiratory syncytial virus (BRSV) 54.1%. Increased relative risk, at 95% confidence intervals, of 1\n                    <jats:sup>st<\/jats:sup>\n                    BRD-treatment failure was associated with positive viral PCR results: BVDV 1.39 (1.17\u20131.66), bPIV-3 1.26 (1.06\u20131.51), BHV-1 1.52 (1.25\u20131.83), and BRSV 1.35 (1.11\u20131.63) from nasal swabs collected at 1\n                    <jats:sup>st<\/jats:sup>\n                    BRD-treatment and culture of\n                    <jats:italic>M<\/jats:italic>\n                    .\n                    <jats:italic>haemolytica<\/jats:italic>\n                    1.23 (1.00\u20131.51) from DNS collected at day-0. However, in this population of high-risk feeder heifers, the predictive values of susceptible and resistant isolates had inadequate association with BRD clinical outcome. These results indicate, that using tulathromycin susceptibility testing of isolates of\n                    <jats:italic>M<\/jats:italic>\n                    .\n                    <jats:italic>haemolytica<\/jats:italic>\n                    or\n                    <jats:italic>P<\/jats:italic>\n                    .\n                    <jats:italic>multocida<\/jats:italic>\n                    from DNS collected on arrival or at 1\n                    <jats:sup>st<\/jats:sup>\n                    BRD-treatment to evaluate tulathromycin clinical efficacy, is unreliable.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0247213","type":"journal-article","created":{"date-parts":[[2022,2,10]],"date-time":"2022-02-10T13:31:45Z","timestamp":1644499905000},"page":"e0247213","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":14,"title":["Limitations of bacterial culture, viral PCR, and tulathromycin susceptibility from upper respiratory tract samples in predicting clinical outcome of tulathromycin control or treatment of bovine respiratory disease in high-risk feeder heifers"],"prefix":"10.1371","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6325-090X","authenticated-orcid":true,"given":"Jeffrey J.","family":"Sarchet","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John P.","family":"Pollreisz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David T.","family":"Bechtol","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitchell R.","family":"Blanding","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roger L.","family":"Saltman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick C.","family":"Taube","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2022,2,10]]},"reference":[{"issue":"3","key":"pone.0247213.ref001","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1016\/S0749-0720(15)30302-9","article-title":"Economic impact associated with respiratory disease in beef cattle","volume":"13","author":"D. Griffin","year":"1997","journal-title":"Vet Clin North Am Food Anim Pract."},{"issue":"12","key":"pone.0247213.ref002","first-page":"1351","article-title":"The epidemiology of bovine respiratory disease: what is the evidence for preventive measures?","volume":"51","author":"JD Taylor","year":"2010","journal-title":"Can Vet J"},{"issue":"2","key":"pone.0247213.ref003","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1016\/j.cvfa.2010.04.004","article-title":"Bacterial pathogens of the bovine respiratory disease complex","volume":"26","author":"D Griffin","year":"2010","journal-title":"Vet Clin North Am Food Anim Pract"},{"key":"pone.0247213.ref004","unstructured":"Smith RA. 2004. Feedlot diseases and their control. In Proc. 23rd World Buiatrics Congr., Quebec City, Canada, Vol. 34. No. 50. Ordre des me\u2019decins veterinaires du Quebec, Quebec, Canada."},{"issue":"2","key":"pone.0247213.ref005","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1017\/S1466252307001326","article-title":"Immune evasion by pathogens of bovine respiratory disease complex","volume":"8","author":"S Srikumaran","year":"2007","journal-title":"Anim Health Res Rev"},{"issue":"2","key":"pone.0247213.ref006","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1016\/j.cvfa.2020.03.002","article-title":"The Immunology of Bovine Respiratory Disease: Recent Advancements","volume":"36","author":"JL McGill","year":"2020","journal-title":"Vet Clin North Am Food Anim Pract"},{"issue":"2","key":"pone.0247213.ref007","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1017\/S1466252314000176","article-title":"Review of BRD pathogenesis: the old and the new","volume":"15","author":"D. 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Temperature With Feedlot Cattle Morbidity and Mortality in the Texas Panhandle","volume":"7","author":"PR Broadway","year":"2020","journal-title":"Front Vet Sci"},{"issue":"3","key":"pone.0247213.ref020","doi-asserted-by":"crossref","first-page":"576","DOI":"10.2527\/jas1984.593576x","article-title":"Shipping suppresses lymphocyte blastogenic responses in Angus and Brahman X Angus feeder calves","volume":"59","author":"F Blecha","year":"1984","journal-title":"J Anim Sci"},{"issue":"1\u20132","key":"pone.0247213.ref021","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/j.vetmic.2008.12.024","article-title":"Respiratory disease in calves: microbiological investigations on trans-tracheally aspirated bronchoalveolar fluid and acute phase protein response","volume":"137","author":"O Angen","year":"2009","journal-title":"Vet Microbiol"},{"issue":"1","key":"pone.0247213.ref022","first-page":"327","article-title":"Comparison of Pasteurella spp. Simultaneously Isolated from Nasal and Transtracheal Swabs from Cattle with Clinical Signs of Bovine Respiratory Disease","volume":"38","author":"D De Rosa","year":"2000","journal-title":"Journal of Microbiology"},{"issue":"1","key":"pone.0247213.ref023","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1136\/vr.160.1.22","article-title":"Use of deep nasopharyngeal swabs as a predictive diagnostic method for natural respiratory infections in calves","volume":"160","author":"KS Godinho","year":"2007","journal-title":"Vet Rec"},{"issue":"4","key":"pone.0247213.ref024","first-page":"341","article-title":"The microbial flora of the respiratory tract in feedlot calves: associations between nasopharyngeal and bronchoalveolar lavage cultures","volume":"55","author":"JW Allen","year":"1991","journal-title":"Can J Vet Res"},{"key":"pone.0247213.ref025","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1016\/j.vetmic.2017.07.013","article-title":"Prevalence and antimicrobial susceptibility of Mannheimia 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Med"},{"issue":"11","key":"pone.0247213.ref028","doi-asserted-by":"crossref","first-page":"e00976","DOI":"10.1128\/MRA.00976-18","article-title":"Closed Genome Sequences and Antibiograms of 16 Pasteurella multocida Isolates from Bovine Respiratory Disease Complex Cases and Apparently Healthy Controls","volume":"7","author":"GP Harhay","year":"2018","journal-title":"Microbiol Resour Announc"},{"issue":"3","key":"pone.0247213.ref029","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1128\/CMR.00047-06","article-title":"Setting and revising antibacterial susceptibility breakpoints","volume":"20","author":"J Turnidge","year":"2007","journal-title":"Clin Microbiol Rev"},{"key":"pone.0247213.ref030","unstructured":"Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing, 27th Ed., CLSI supplement 100, Wayne PA: 2017, ISBN 1-56238-805-3, vol 37, no. 1, https:\/\/clsi.org\/media\/3481\/m100ed30_sample.pdf."},{"issue":"9 Suppl","key":"pone.0247213.ref031","first-page":"S11","article-title":"The Clinical Predictive Value (or Lack Thereof) of the Results of In Vitro Antimicrobial Susceptibility Tests","volume":"49","author":"GV Doern","year":"2011","journal-title":"J Clin Microbiol"},{"issue":"8","key":"pone.0247213.ref032","doi-asserted-by":"crossref","first-page":"982","DOI":"10.1086\/342384","article-title":"Has antifungal susceptibility testing come of age?","volume":"35","author":"JH Rex","year":"2002","journal-title":"Clin Infect Dis"},{"issue":"9","key":"pone.0247213.ref033","doi-asserted-by":"crossref","first-page":"1169","DOI":"10.1016\/j.ebiom.2015.08.012","article-title":"Host-dependent Induction of Transient Antibiotic Resistance: A Prelude to Treatment Failure","volume":"2","author":"J. Z. Kubicek-Sutherland","year":"2015","journal-title":"EBioMedicine"},{"issue":"4","key":"pone.0247213.ref034","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1089\/mdr.2005.11.342","article-title":"Assessment of accuracy of disk diffusion tests for the determination of antimicrobial susceptibility of common bovine mastitis pathogens: a novel approach","volume":"11","author":"E Klement","year":"2005","journal-title":"Microb Drug Resist"},{"key":"pone.0247213.ref035","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/j.ebiom.2017.05.026","article-title":"Correcting a Fundamental Flaw in the Paradigm for Antimicrobial Susceptibility Testing","volume":"20","author":"S. C. Ersoy","year":"2017","journal-title":"EBioMedicine"},{"issue":"1","key":"pone.0247213.ref036","doi-asserted-by":"crossref","first-page":"1","DOI":"10.21423\/bovine-vol51no1p1-13","article-title":"Antimicrobial drug use for control and treatment of bovine respiratory disease in US feedlot cattle: A meta-analysis of comparative studies versus tulathromycin","volume":"51","author":"BP Nautrup","year":"2017","journal-title":"The Bovine Practitioner"},{"issue":"2","key":"pone.0247213.ref037","first-page":"626","article-title":"A mixed treatment comparison meta-analysis of metaphylaxis treatments for bovine respiratory disease in beef cattle","volume":"95","author":"KM Abell","year":"2017","journal-title":"J Anim Sci"},{"key":"pone.0247213.ref038","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1016\/j.prevetmed.2016.07.003","article-title":"A mixed treatment meta-analysis of antibiotic treatment options for bovine respiratory disease\u2014An update","volume":"132","author":"AM O\u2019Connor","year":"2016","journal-title":"Prev Vet Med"},{"key":"pone.0247213.ref039","doi-asserted-by":"crossref","first-page":"297","DOI":"10.3389\/fmicb.2013.00297","article-title":"Susceptibility to tulathromycin in Mannheimia haemolytica isolated from feedlot cattle over a 3-year period","volume":"4","author":"TW Alexander","year":"2013","journal-title":"Front Microbiol"},{"key":"pone.0247213.ref040","unstructured":"Leary S, Underwood W, Anthony R, Carter S, Grandin T, Greenacre C, et al. AVMA Guidelines for the Euthanasia of Animals: 2013 Edition, ISBN 978-1-882691-21-0. https:\/\/www.avma.org\/KB\/Policies\/documents\/euthanasia.pdf."},{"key":"pone.0247213.ref041","unstructured":"Beef Quality Assurance, ABOUT US,INTRODUCTION TO BQA,WHAT IS BQA?, https:\/\/www.bqa.org\/about-us\/introduction-to-bqa, accessed 30 March 2021. Accessed 31March2021."},{"issue":"3","key":"pone.0247213.ref042","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1177\/201010581001900316","article-title":"The Correct Interpretation of Confidence Intervals","volume":"19","author":"S Hue Tan","year":"2010","journal-title":"Proceeding of Singapore Healthcare"},{"key":"pone.0247213.ref043","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.rvsc.2014.12.015","article-title":"Nasal isolation of Mannheimia haemolytica and Pasteurella multocida as predictors of respiratory disease in shipped calves","volume":"99","author":"JD Taylor","year":"2015","journal-title":"Res Vet Sci"},{"issue":"1","key":"pone.0247213.ref044","doi-asserted-by":"crossref","first-page":"129","DOI":"10.2460\/javma.239.1.129","article-title":"Relationship of in vitro minimum inhibitory concentrations of tilmicosin against Mannheimia haemolytica and Pasteurella multocida and in vivo tilmicosin treatment outcome among calves with signs of bovine respiratory disease","volume":"239","author":"DG McClary","year":"2011","journal-title":"J Am Vet Med Assoc"},{"issue":"6","key":"pone.0247213.ref045","doi-asserted-by":"crossref","first-page":"507","DOI":"10.2460\/ajvr.76.6.507","article-title":"Immunomodulatory effects of tulathromycin on apoptosis, efferocytosis, and proinflammatory leukotriene B4 production in leukocytes from Actinobacillus pleuropneumoniae-or zymosan-challenged pigs","volume":"76","author":"SC Duquette","year":"2015","journal-title":"Am J Vet Res"},{"issue":"4","key":"pone.0247213.ref046","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1556\/avet.2012.041","article-title":"Effects of tylosin, tilmicosin and tulathromycin on inflammatory mediators in bronchoalveolar lavage fluid of lipopolysaccharide-induced lung injury","volume":"60","author":"A Er","year":"2012","journal-title":"Acta Vet 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Holstein calves following a single subcutaneous administration of 2.5 mg\/kg body weight","volume":"8","author":"S Cox","year":"2010","journal-title":"International Journal of Applied Research in Veterinary Medicine"},{"issue":"1","key":"pone.0247213.ref050","first-page":"60","article-title":"Pharmacokinetics and lung tissue concentrations of tulathromycin, a new triamilide antibiotic, in cattle","volume":"5","author":"MA Nowakowski","year":"2004","journal-title":"Vet Ther"},{"issue":"3","key":"pone.0247213.ref051","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1128\/AAC.42.3.521","article-title":"Pharmacodynamic evaluation of factors associated with the development of bacterial resistance in acutely ill patients during therapy","volume":"42","author":"JK Thomas","year":"1998","journal-title":"Antimicrob Agents 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