{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T03:11:44Z","timestamp":1792293104729,"version":"4.4.0"},"reference-count":50,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2021,2,22]],"date-time":"2021-02-22T00:00:00Z","timestamp":1613952000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"One Health Graduate Training Award, University of Calgary"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Livestock farming plays an important role in supporting the livelihood of resource-poor subsistence farmers in Bhutan. However, ticks and tick-borne diseases (TBDs) are one of the major constraints to livestock farming due to their negative effect on health and production. To date, no study has been conducted in Bhutan to assess farmers\u2019 knowledge, attitude, and practices (KAP) about ticks and TBDs in cattle, although such information is essential in ensuring the development and adoption of effective prevention and control measures. Therefore, a KAP survey was conducted among 246 cattle owners in the Samkhar sub-district of eastern Bhutan in June 2019, using a structured questionnaire. Based on our scoring criteria, 52% [95%CI: 45.5\u201358.4] had adequate knowledge about ticks as potential vectors of diseases. Logistic regression analysis showed that the individuals who practiced a stall-feeding system of cattle rearing were 2.8 times [OR = 2.8 (95%CI: 1.66\u20134.78)] more likely to have adequate knowledge than others. Sixty-eight percent [95%CI: 62.5\u201374.4] had a favorable attitude toward tick prevention and control programs. Men were 1.95 times [OR = 1.95 (95%CI: 1.09\u20133.55)] more likely to have a favorable attitude than women, and the individuals who practiced a stall-feeding system were 2.59 times [OR = 2.59 95%CI: 1.45\u20134.78)] more likely to have a favorable attitude than others, after adjusting for the effect of other variables in the model. Overall, only 38% [95%CI 32.5\u201345] of the respondents reported tick infestation as one of the most important animal health problems, but 100% reported using acaricides to control ticks in cattle. Despite a high level of acaricide usage, the level of knowledge was low among the farmers interviewed. Findings from this study underline the importance of considering identified knowledge gaps and initiating education efforts to improve the adoption of effective tick prevention and control measures among farmers.<\/jats:p>","DOI":"10.1371\/journal.pone.0247302","type":"journal-article","created":{"date-parts":[[2021,2,22]],"date-time":"2021-02-22T13:30:03Z","timestamp":1614000603000},"page":"e0247302","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":42,"title":["A knowledge, attitudes, and practices study on ticks and tick-borne diseases in cattle among farmers in a selected area of eastern Bhutan"],"prefix":"10.1371","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4889-478X","authenticated-orcid":true,"given":"Jamyang","family":"Namgyal","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tenzin","family":"Tenzin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvia","family":"Checkley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tim J.","family":"Lysyk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sangay","family":"Rinchen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ratna B.","family":"Gurung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sithar","family":"Dorjee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Couloigner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8200-3584","authenticated-orcid":true,"given":"Susan C.","family":"Cork","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2021,2,22]]},"reference":[{"key":"pone.0247302.ref001","unstructured":"National Statistics Bureau. 2017 population & housing census of Bhutan: national report = \u02bcBrug gi mi rlobs dang khyim gyi grangs rtsis 2017. 2018. Available from: http:\/\/www.nsb.gov.bt\/publication\/files\/PHCB2017_national.pdf"},{"key":"pone.0247302.ref002","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.prevetmed.2016.05.012","article-title":"A cross-sectional survey of population demographics, the prevalence of major disease conditions and reason-specific proportional mortality of domestic cattle in the Kingdom of Bhutan","volume":"130","author":"A Hidano","year":"2016","journal-title":"Prev Vet Med"},{"key":"pone.0247302.ref003","unstructured":"Phanchung, Dorji P, Sonam T, Pelden K. Smallholder Dairy Farming in Bhutan: Characteristics, Constraints, and Development Opportunities. 2007. Available from: http:\/\/lib.icimod.org\/record\/21365\/files\/c_attachment_79_552.pdf"},{"key":"pone.0247302.ref004","unstructured":"NCAH. Status of Notifiable Animal Diseases in Bhutan, 2011\u20132012. 2013 [cited 19 Jan 2021]. Available from: https:\/\/www.ncah.gov.bt\/download_reports.php"},{"key":"pone.0247302.ref005","unstructured":"Minjauw B, McLeod A. Tick-borne diseases and poverty. The impact of ticks and tickborne diseases on the livelihood of small-scale and marginal livestock owners in India and eastern and southern Africa. 2003 [cited 16 Feb 2020]. Available: http:\/\/agris.fao.org\/agris-search\/search.do?recordID=GB2012100456"},{"key":"pone.0247302.ref006","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/S0304-4017(97)00033-2","article-title":"Sustainable tick and tickborne disease control in livestock improvement in developing countries","volume":"71","author":"JJ de Castro","year":"1997","journal-title":"Vet Parasitol"},{"key":"pone.0247302.ref007","unstructured":"Estrada-Pena A, Mans BJ. Tick-induced paralysis and toxicoses. illustrated, revised ed. Page 313 Biology of Ticks Volume 2. illustrated, revised ed. OUP USA, 2013; 2013. p. 496."},{"key":"pone.0247302.ref008","doi-asserted-by":"crossref","first-page":"117","DOI":"10.4103\/2224-3151.206248","article-title":"Scrub typhus in Bhutan: a synthesis of data from 2009 to 2014","volume":"5","author":"T Tshokey","year":"2016","journal-title":"WHO South-East Asia J Public Health"},{"key":"pone.0247302.ref009","doi-asserted-by":"crossref","first-page":"e0006107","DOI":"10.1371\/journal.pntd.0006107","article-title":"Seroprevalence of rickettsial infections and Q fever in Bhutan","volume":"11","author":"T Tshokey","year":"2017","journal-title":"PLoS Negl Trop Dis"},{"key":"pone.0247302.ref010","doi-asserted-by":"crossref","first-page":"417","DOI":"10.4103\/0971-5916.159279","article-title":"DHR-ICMR Guidelines for diagnosis & management of Rickettsial diseases in India","volume":"141","author":"M Rahi","year":"2015","journal-title":"Indian J Med Res"},{"key":"pone.0247302.ref011","doi-asserted-by":"crossref","first-page":"1108","DOI":"10.1111\/j.1469-0691.2004.01019.x","article-title":"Guidelines for the diagnosis of tick-borne bacterial diseases in Europe","volume":"10","author":"P Brouqui","year":"2004","journal-title":"Clin Microbiol Infect"},{"key":"pone.0247302.ref012","first-page":"35","volume-title":"External parasiticides. 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PHCB Trashigang. 2018. Available from: http:\/\/www.nsb.gov.bt\/publication\/publications.php?id=2"},{"key":"pone.0247302.ref024","unstructured":"DoL. Livestock Statistics. Department of Livestock, Ministry of Agriculture and Forests, Royal Government of Bhutan; 2018. Available from: http:\/\/www.dol.gov.bt\/?page_id=394"},{"key":"pone.0247302.ref025","doi-asserted-by":"crossref","first-page":"1279","DOI":"10.2460\/javma.242.9.1279","article-title":"Evaluation of knowledge, attitudes, and practices of deer owners following identification of a cluster of captive deer with rabies in Pennsylvania in July 2010","volume":"242","author":"DM Tack","year":"2013","journal-title":"Javma-J Am Vet Med Assoc"},{"key":"pone.0247302.ref026","doi-asserted-by":"crossref","first-page":"e102028","DOI":"10.1371\/journal.pone.0102028","article-title":"Knowledge, Attitude and Practice Regarding Dengue Fever among the Healthy Population of Highland and Lowland Communities in Central Nepal","volume":"9","author":"M Dhimal","year":"2014","journal-title":"PLOS ONE"},{"key":"pone.0247302.ref027","doi-asserted-by":"crossref","first-page":"e0007305","DOI":"10.1371\/journal.pntd.0007305","article-title":"A community-based knowledge, attitude, and practice survey on rabies among cattle owners in selected areas of Bhutan","volume":"13","author":"S Rinchen","year":"2019","journal-title":"PLoS Negl Trop Dis"},{"key":"pone.0247302.ref028","doi-asserted-by":"crossref","first-page":"e0117318","DOI":"10.1371\/journal.pone.0117318","article-title":"A Study of Knowledge, Attitudes and Practices Relating to Brucellosis among Small-Scale Dairy Farmers in an Urban and Peri-Urban Area of Tajikistan","volume":"10","author":"E Lindahl","year":"2015","journal-title":"PLOS ONE"},{"key":"pone.0247302.ref029","unstructured":"Allison P. When Can You Safely Ignore Multicollinearity? In: When Can You Safely Ignore Multicollinearity? [Internet]. 2012. Available from: https:\/\/statisticalhorizons.com\/multicollinearity"},{"key":"pone.0247302.ref030","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1186\/s40066-018-0229-6","article-title":"Climate change and potential impacts on agriculture in Bhutan: a discussion of pertinent issues","volume":"7","author":"N Chhogyel","year":"2018","journal-title":"Agric Food Secur"},{"key":"pone.0247302.ref031","doi-asserted-by":"crossref","first-page":"53","DOI":"10.20506\/rst.34.1.2345","article-title":"Ticks as vectors: taxonomy, biology and ecology: -EN- -FR- Les tiques en tant que vecteurs: taxonomie, biologie et \u00e9cologie -ES- Las garrapatas como vectores: taxonom\u00eda, biolog\u00eda y ecolog\u00eda","volume":"34","author":"A. Estrada-Pe\u00f1a","year":"2015","journal-title":"Rev Sci Tech OIE"},{"key":"pone.0247302.ref032","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1111\/j.1708-8305.2007.00176.x","article-title":"What Makes Ticks Tick? Climate Change, Ticks, and Tick\u2010Borne Diseases","volume":"15","author":"J S\u00fcss","year":"2008","journal-title":"J Travel Med"},{"key":"pone.0247302.ref033","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1080\/09291016.2017.1395527","article-title":"Prevalence of hard tick infestations in cattle of West Bengal, India","volume":"49","author":"A Debbarma","year":"2018","journal-title":"Biol Rhythm Res"},{"key":"pone.0247302.ref034","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1111\/pim.12140","article-title":"Host resistance in cattle to infestation with the cattle tick Rhipicephalus microplus","volume":"36","author":"NN Jonsson","year":"2014","journal-title":"Parasite Immunol"},{"key":"pone.0247302.ref035","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.livsci.2010.05.014","article-title":"Crossbreeding and intensification of smallholder crop-cattle farming systems in Bhutan","volume":"132","author":"T Samdup","year":"2010","journal-title":"Livest Sci"},{"key":"pone.0247302.ref036","first-page":"346","article-title":"An epidemiological survey on investigation of tick infestation in cattle at Chittagong District, Bangladesh","volume":"5","author":"MHB Kabir","year":"2011","journal-title":"Afr J Microbiol Res"},{"key":"pone.0247302.ref037","doi-asserted-by":"crossref","DOI":"10.1155\/2016\/9618291","article-title":"Infestation and Identification of Ixodid Tick in Cattle: The Case of Arbegona District, Southern Ethiopia","volume":"2016","author":"J Kemal","year":"2016","journal-title":"J Vet Med"},{"key":"pone.0247302.ref038","first-page":"479","article-title":"Survey of tick infestation of cattle at four selected grazing sites in the tropics","volume":"12","author":"JE Eyo","year":"2014","journal-title":"Glob Vet"},{"key":"pone.0247302.ref039","unstructured":"Spickler AR. Bovine Babesiosis. 2018 [cited 15 Jul 2020]. Available from: http:\/\/www.cfsph.iastate.edu\/DiseaseInfo\/factsheets.php"},{"key":"pone.0247302.ref040","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.prevetmed.2017.02.011","article-title":"Revisiting an old disease? Risk factors for bovine enzootic haematuria in the Kingdom of Bhutan","volume":"140","author":"A Hidano","year":"2017","journal-title":"Prev Vet Med"},{"key":"pone.0247302.ref041","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1186\/s13071-017-2138-0","article-title":"Distribution of ticks infesting ruminants and risk factors associated with high tick prevalence in livestock farms in the semi-arid and arid agro-ecological zones of Pakistan","volume":"10","author":"A Rehman","year":"2017","journal-title":"Parasit Vectors"},{"key":"pone.0247302.ref042","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1080\/10357820308713382","article-title":"Can a nationalised forest management system uphold local institutions? The case of leaf litter forest [sokshing] management in Bhutan","volume":"27","author":"L Dorji","year":"2003","journal-title":"Asian Stud Rev"},{"key":"pone.0247302.ref043","doi-asserted-by":"crossref","DOI":"10.3390\/insects10080227","article-title":"Impacts of Deciduous Leaf Litter and Snow Presence on Nymphal Ixodes scapularis (Acari: Ixodidae) Overwintering Survival in Coastal New England, USA","volume":"10","author":"MA Linske","year":"2019","journal-title":"Insects"},{"key":"pone.0247302.ref044","article-title":"Knowledge and perception on ticks, tick-borne diseases and indigenous cattle tolerance to East Coast fever in agro-pastoral communities of Lake Zone in Tanzania","volume":"27","author":"E Laisser","year":"2015","journal-title":"Livest Res Rural Dev"},{"key":"pone.0247302.ref045","unstructured":"Kuensel. Saga Dawa, the auspicious month. 2016 [cited 5 Mar 2020]. Available from: https:\/\/kuenselonline.com\/saga-dawa-the-auspicious-month\/"},{"key":"pone.0247302.ref046","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1590\/S1984-29612013005000006","article-title":"Acaricides efficiency on Rhipicephalus(Boophilus) microplus from Bahia state North-Central region","volume":"22","author":"JT Raynal","year":"2013","journal-title":"Rev Bras Parasitol Veterin\u00e1ria"},{"key":"pone.0247302.ref047","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1016\/j.jep.2018.08.010","article-title":"Zanthoxylum armatum DC.: Current knowledge, gaps and opportunities in Nepal","volume":"229","author":"N Phuyal","year":"2019","journal-title":"J Ethnopharmacol"},{"key":"pone.0247302.ref048","first-page":"353","article-title":"Documentation of ethnoveterinary practices used for treatment of different ailments in a selected hilly area of Pakistan","volume":"12","author":"Z-D Sindhu","year":"2010","journal-title":"Int J Agric Biol"},{"key":"pone.0247302.ref049","doi-asserted-by":"crossref","first-page":"971","DOI":"10.1603\/ME13236","article-title":"Acaricidal Properties of the Essential Oil From Zanthoxylum caribaeum Against Rhipicephalus microplus","volume":"51","author":"J Nogueira","year":"2014","journal-title":"J Med Entomol"},{"key":"pone.0247302.ref050","doi-asserted-by":"crossref","first-page":"414","DOI":"10.1071\/AR9570414","article-title":"The spelling of pasture in cattle tick control","volume":"8","author":"PR Wilkinson","year":"1957","journal-title":"Aust J Agric Res"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0247302","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,2,22]],"date-time":"2021-02-22T13:30:54Z","timestamp":1614000654000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0247302"}},"subtitle":[],"editor":[{"given":"Simon","family":"Clegg","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2021,2,22]]},"references-count":50,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2021,2,22]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0247302","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,2,22]]}}}