{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T03:47:03Z","timestamp":1782877623463,"version":"3.54.5"},"reference-count":10,"publisher":"Public Library of Science (PLoS)","issue":"2","license":[{"start":{"date-parts":[[2021,2,24]],"date-time":"2021-02-24T00:00:00Z","timestamp":1614124800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>\n                    The outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) rapidly reached pandemic levels. Sufficient testing for SARS-CoV-2 has remained essential for tracking and containing the virus. SARS-CoV-2 testing capabilities are still limited in many countries. Here, we explore the use of conventional RNA purification as an alternative to automated systems for detection of SARS-CoV-2 by RT-qPCR. 87 clinical swab specimens were extracted by conventional phenol-chloroform RNA purification and compared to commercial platforms for RNA extraction and the fully integrated Cobas\n                    <jats:sup>\u00ae<\/jats:sup>\n                    6800 diagnostic system. Our results show that the conventional RNA extraction is fully comparable to modern automated systems regarding analytical sensitivity and specificity with respect to detection of SARS-CoV-2 as evaluated by RT-qPCR. Moreover, the method is easily scalable and implemented in conventional laboratories as a low cost and suitable alternative to automated systems for the detection of SARS-CoV-2.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0247524","type":"journal-article","created":{"date-parts":[[2021,2,24]],"date-time":"2021-02-24T13:33:23Z","timestamp":1614173603000},"page":"e0247524","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":11,"title":["Phenol-chloroform-based RNA purification for detection of SARS-CoV-2 by RT-qPCR: Comparison with automated systems"],"prefix":"10.1371","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9170-2168","authenticated-orcid":true,"given":"Henrik","family":"Dimke","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanne 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coronavirus in Wuhan, China","volume":"395","author":"C Huang","year":"2020","journal-title":"Lancet"},{"issue":"10229","key":"pone.0247524.ref002","doi-asserted-by":"crossref","first-page":"1054","DOI":"10.1016\/S0140-6736(20)30566-3","article-title":"Clinical course and risk factors for mortality of adult inpatients with COVID-19 in Wuhan, China: a retrospective cohort study","volume":"395","author":"F Zhou","year":"2020","journal-title":"Lancet"},{"issue":"8","key":"pone.0247524.ref003","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1056\/NEJMoa2001017","article-title":"A Novel Coronavirus from Patients with Pneumonia in China, 2019","volume":"382","author":"N Zhu","year":"2020","journal-title":"N Engl J Med"},{"key":"pone.0247524.ref004","article-title":"Development of a Laboratory-safe and Low-cost Detection Protocol for SARS-CoV-2 of the Coronavirus Disease 2019 (COVID-19)","author":"J Won","year":"2020","journal-title":"Exp 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Surveill"},{"key":"pone.0247524.ref008","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.jviromet.2015.07.002","article-title":"Inactivation and safety testing of Middle East Respiratory Syndrome Coronavirus","volume":"223","author":"M Kumar","year":"2015","journal-title":"J Virol Methods"},{"issue":"2","key":"pone.0247524.ref009","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/j.jviromet.2004.03.015","article-title":"Virus inactivation by nucleic acid extraction reagents","volume":"119","author":"JA Blow","year":"2004","journal-title":"J Virol Methods"},{"key":"pone.0247524.ref010","article-title":"Phenol-chloroform-based RNA purification for detection of SARS-CoV-2 by RT-qPCR: comparison with automated systems","author":"H Dimke","year":"2020","journal-title":"MedRxiv"}],"container-title":["PLOS 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