{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T00:37:30Z","timestamp":1792802250545,"version":"4.5.0"},"reference-count":33,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2021,3,3]],"date-time":"2021-03-03T00:00:00Z","timestamp":1614729600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"government-wide R&D fund project for infectious disease research","award":["HG18C0012"],"award-info":[{"award-number":["HG18C0012"]}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"crossref","award":["NRF-2016R1A5A1010148"],"award-info":[{"award-number":["NRF-2016R1A5A1010148"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"crossref"}]},{"name":"the Ministry of Health & Welfare, Republic of Korea","award":["HR20C0021"],"award-info":[{"award-number":["HR20C0021"]}],"id":[{"id":"https:\/\/ror.org\/00vxgjw72","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>A newly identified coronavirus, designated as severe acute respiratory syndrome coronavirus 2 (SARS CoV-2), has spread rapidly from its epicenter in China to more than 150 countries across six continents. In this study, we have designed three reverse-transcription loop-mediated isothermal amplification (RT-LAMP) primer sets to detect the RNA-dependent RNA polymerase (RdRP), Envelope (E) and Nucleocapsid protein (N) genes of SARS CoV-2. For one tube reaction, the detection limits for five combination SARS CoV-2 LAMP primer sets (RdRP\/E, RdRP\/N, E\/N, RdRP\/E\/N and RdRP\/N\/Internal control (actin beta)) were evaluated with a clinical nasopharyngeal swab sample. Among the five combination, the RdRP\/E and RdRP\/N\/IC multiplex LAMP assays showed low detection limits. The sensitivity and specificity of the RT-LAMP assay were evaluated and compared to that of the widely used Allplex\u2122 2019-nCoV Assay (Seegene, Inc., Seoul, South Korea) and PowerChek\u2122 2019-nCoV Real-time PCR kit (Kogenebiotech, Seoul, South Korea) for 130 clinical samples from 91 SARS CoV-2 patients and 162 NP specimens from individuals with (72) and without (90) viral respiratory infections. The multiplex RdRP (FAM)\/N (CY5)\/IC (Hex) RT-LAMP assay showed comparable sensitivities (RdRP: 93.85%, N: 94.62% and RdRP\/N: 96.92%) to that of the Allplex\u2122 2019-nCoV Assay (100%) and superior to those of PowerChek\u2122 2019-nCoV Real-time PCR kit (RdRP: 92.31%, E: 93.85% and RdRP\/E: 95.38%).<\/jats:p>","DOI":"10.1371\/journal.pone.0248042","type":"journal-article","created":{"date-parts":[[2021,3,3]],"date-time":"2021-03-03T14:42:03Z","timestamp":1614782523000},"page":"e0248042","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":68,"title":["Development of a multiplex Loop-Mediated Isothermal Amplification (LAMP) assay for on-site diagnosis of SARS CoV-2"],"prefix":"10.1371","volume":"16","author":[{"given":"Woong Sik","family":"Jang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Da 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