{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T22:05:16Z","timestamp":1790719516512,"version":"4.1.0"},"reference-count":21,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2021,3,10]],"date-time":"2021-03-10T00:00:00Z","timestamp":1615334400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:sec id=\"sec001\">\n<jats:title>Background<\/jats:title>\n<jats:p>Gut colonization by ESBL-producing <jats:italic>Enterobacteriaceae<\/jats:italic> (ESBL-PE) is widespread and is promoted by antibiotic exposure. Higher fecal abundance of ESBL-PE promotes the dissemination of the bacteria in the environment and is associated with increased risk of infection. Ceftriaxone and temocillin are commonly used antibiotics with a different activity on gut flora. Their impact on fecal abundance of ESBL-producing <jats:italic>Enterobacteriaceae<\/jats:italic> has not been studied. The objective of this study was to compare the propensity of ceftriaxone and temocillin to modify the abundance of ESBL-producing <jats:italic>Escherichia coli<\/jats:italic> in feces of colonized mice.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec002\">\n<jats:title>Methods<\/jats:title>\n<jats:p>Mice received broad-spectrum antibiotics in order to disrupt their normal gut flora. A CTX-M-type ESBL-producing <jats:italic>E<\/jats:italic>. <jats:italic>coli<\/jats:italic> clinical isolate was then administered orally, leading to durable colonization. Thirty days later, mice received either temocillin or ceftriaxone with drinking water at a concentration simulating human intestinal exposure. Third-generation-cephalosporin resistant (3GCR) <jats:italic>E<\/jats:italic>. <jats:italic>coli<\/jats:italic> were enumerated in feces on selective medium before, 2 days and 10 days after the end of antibiotic exposure. The experiment was performed with two <jats:italic>E<\/jats:italic>. <jats:italic>coli<\/jats:italic> isolates with different temocillin minimum inhibitory concentrations.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec003\">\n<jats:title>Results<\/jats:title>\n<jats:p>Exposure to ceftriaxone induced an increase in the fecal abundance of 3GCR <jats:italic>E<\/jats:italic>. <jats:italic>coli<\/jats:italic>. In contrast, temocillin had no effect or transiently decreased the number of 3GCR <jats:italic>E<\/jats:italic>. <jats:italic>coli<\/jats:italic>. Results obtained with the two strains were similar.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec004\">\n<jats:title>Conclusion<\/jats:title>\n<jats:p>Contrary to ceftriaxone, temocillin does not promote expansion of ESBL-producing <jats:italic>E<\/jats:italic>. <jats:italic>coli<\/jats:italic> in feces of colonized mice. Thus temocillin may be a therapeutic of choice when a temocillin-susceptible strain infection is suspected or proven to prevent the expansion of ESBL-PE in a previously colonized patient.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pone.0248177","type":"journal-article","created":{"date-parts":[[2021,3,10]],"date-time":"2021-03-10T18:40:30Z","timestamp":1615401630000},"page":"e0248177","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":17,"title":["Impact of ceftriaxone and temocillin on fecal abundance of extended-spectrum \u03b2-lactamase producing Escherichia coli in a mouse model"],"prefix":"10.1371","volume":"16","author":[{"given":"Rachel","family":"Chenouard","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael","family":"Mahieu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Luque Paz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4917-9665","authenticated-orcid":true,"given":"Estelle","family":"Marion","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthieu","family":"Eveillard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9982-4741","authenticated-orcid":true,"given":"Vincent","family":"Dub\u00e9e","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2021,3,10]]},"reference":[{"key":"pone.0248177.ref001","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1093\/cid\/ciw283","article-title":"Fecal Colonization With Extended-spectrum Beta-lactamase-Producing Enterobacteriaceae and Risk Factors Among Healthy Individuals: A Systematic Review and Metaanalysis","volume":"63","author":"S Karanika","year":"2016","journal-title":"Clin Infect Dis"},{"key":"pone.0248177.ref002","doi-asserted-by":"crossref","first-page":"1431","DOI":"10.1017\/ice.2018.254","article-title":"Poor clinical outcomes associated with community-onset urinary tract infections due to extended-spectrum cephalosporin-resistant Enterobacteriaceae","volume":"39","author":"JA Anesi","year":"2018","journal-title":"Infect Control Hosp Epidemiol"},{"key":"pone.0248177.ref003","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1016\/j.jinf.2007.04.007","article-title":"Mortality following bacteraemic infection caused by extended spectrum beta-lactamase (ESBL) producing E. coli compared to non-ESBL producing E. coli","volume":"55","author":"M Melzer","year":"2007","journal-title":"J Infect"},{"key":"pone.0248177.ref004","doi-asserted-by":"crossref","first-page":"4512","DOI":"10.1128\/AAC.00238-13","article-title":"Relative fecal abundance of extended-spectrum-\u03b2-lactamase-producing Escherichia coli strains and their occurrence in urinary tract infections in women","volume":"57","author":"E Rupp\u00e9","year":"2013","journal-title":"Antimicrob Agents Chemother"},{"key":"pone.0248177.ref005","doi-asserted-by":"crossref","first-page":"129","DOI":"10.3389\/fmicb.2013.00129","article-title":"Causes, consequences, and perspectives in the variations of intestinal density of colonization of multidrug-resistant enterobacteria","volume":"4","author":"E Rupp\u00e9","year":"2013","journal-title":"Front Microbiol"},{"key":"pone.0248177.ref006","doi-asserted-by":"crossref","first-page":"6941","DOI":"10.1128\/AAC.01328-16","article-title":"Prospective Cohort Study of the Relative Abundance of Extended-Spectrum-Beta-Lactamase-Producing Escherichia coli in the Gut of Patients Admitted to Hospitals","volume":"60","author":"V de Lastours","year":"2016","journal-title":"Antimicrob Agents Chemother"},{"key":"pone.0248177.ref007","doi-asserted-by":"crossref","first-page":"3044","DOI":"10.1128\/AAC.00194-07","article-title":"Effects of imipenem-cilastatin, ertapenem, piperacillin-tazobactam, and ceftriaxone treatments on persistence of intestinal colonization by extended-spectrum-beta-lactamase-producing Klebsiella pneumoniae strains in mice","volume":"51","author":"MJ Pultz","year":"2007","journal-title":"Antimicrob Agents Chemother"},{"key":"pone.0248177.ref008","doi-asserted-by":"crossref","first-page":"478","DOI":"10.1016\/j.ijantimicag.2019.06.015","article-title":"Comparison of antibiotic treatment guidelines for urinary tract infections in 15 European countries: Results of an online survey","volume":"54","author":"K Malmros","year":"2019","journal-title":"Int J Antimicrob Agents"},{"key":"pone.0248177.ref009","article-title":"Levels of evidence supporting European and American community-acquired pneumonia guidelines","author":"J Ferreira-Coimbra","year":"2020","journal-title":"Eur J Clin Microbiol Infect Dis"},{"key":"pone.0248177.ref010","doi-asserted-by":"crossref","first-page":"1466","DOI":"10.1093\/jac\/dku542","article-title":"Activity of temocillin in a murine model of urinary tract infection due to Escherichia coli producing or not producing the ESBL CTX-M-15","volume":"70","author":"JF Soubirou","year":"2015","journal-title":"J Antimicrob Chemother"},{"key":"pone.0248177.ref011","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1128\/AAC.33.2.204","article-title":"Imipenem concentrations in colorectal surgery and impact on the colonic microflora","volume":"33","author":"L Kager","year":"1989","journal-title":"Antimicrob Agents Chemother"},{"key":"pone.0248177.ref012","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1007\/BF02148656","article-title":"Interpretive criteria for temocillin disk diffusion susceptibility testing","volume":"4","author":"PC Fuchs","year":"1985","journal-title":"Eur J Clin Microbiol"},{"key":"pone.0248177.ref013","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.jinf.2017.04.008","article-title":"Impact of faecal microbiota transplantation on mouse digestive colonization with two extensively resistant bacteria","volume":"75","author":"R Mahieu","year":"2017","journal-title":"J Infect"},{"key":"pone.0248177.ref014","first-page":"1","article-title":"Iterative Fecal Microbiota Transplantations for Eradicating Digestive Colonization With Carbapenemase-Producing Enterobacteriaceae: Is It Worth It?","author":"R Mahieu","year":"2017","journal-title":"Infect Control Hosp Epidemiol"},{"key":"pone.0248177.ref015","doi-asserted-by":"crossref","first-page":"3765","DOI":"10.1128\/AAC.48.10.3765-3772.2004","article-title":"Ertapenem pharmacokinetics and impact on intestinal microflora, in comparison to those of ceftriaxone, after multiple dosing in male and female volunteers","volume":"48","author":"MWR Pletz","year":"2004","journal-title":"Antimicrob Agents Chemother"},{"key":"pone.0248177.ref016","doi-asserted-by":"crossref","first-page":"3610","DOI":"10.1128\/AAC.47.11.3610-3612.2003","article-title":"Effect of parenteral antibiotic administration on establishment of intestinal colonization in mice by Klebsiella pneumoniae strains producing extended-spectrum beta-lactamases","volume":"47","author":"CK Hoyen","year":"2003","journal-title":"Antimicrob Agents Chemother"},{"key":"pone.0248177.ref017","doi-asserted-by":"crossref","first-page":"949","DOI":"10.1086\/428090","article-title":"Mechanisms by which anaerobic microbiota inhibit the establishment in mice of intestinal colonization by vancomycin-resistant Enterococcus","volume":"191","author":"NJ Pultz","year":"2005","journal-title":"J Infect Dis"},{"key":"pone.0248177.ref018","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1007\/s10096-005-1356-0","article-title":"Bowel colonization with resistant gram-negative bacilli after antimicrobial therapy of intra-abdominal infections: observations from two randomized comparative clinical trials of ertapenem therapy","volume":"24","author":"MJ Dinubile","year":"2005","journal-title":"Eur J Clin Microbiol Infect Dis"},{"key":"pone.0248177.ref019","doi-asserted-by":"crossref","first-page":"1925","DOI":"10.1056\/NEJM200012283432604","article-title":"Effect of antibiotic therapy on the density of vancomycin-resistant enterococci in the stool of colonized patients","volume":"343","author":"CJ Donskey","year":"2000","journal-title":"N Engl J Med"},{"key":"pone.0248177.ref020","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/j.medmal.2018.03.005","article-title":"Practice guidelines for the management of adult community-acquired urinary tract infections","volume":"48","author":"F Caron","year":"2018","journal-title":"M\u00e9decine et Maladies Infectieuses"},{"key":"pone.0248177.ref021","doi-asserted-by":"crossref","first-page":"1848","DOI":"10.1093\/jac\/dky101","article-title":"Temocillin against Enterobacteriaceae isolates from community-acquired urinary tract infections: low rate of resistance and good accuracy of routine susceptibility testing methods","volume":"73","author":"K Alexandre","year":"2018","journal-title":"J Antimicrob Chemother"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0248177","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,3,10]],"date-time":"2021-03-10T18:41:02Z","timestamp":1615401662000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0248177"}},"subtitle":[],"editor":[{"given":"Monica","family":"Cartelle Gestal","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2021,3,10]]},"references-count":21,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2021,3,10]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0248177","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,3,10]]}}}