{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:48:12Z","timestamp":1792788492453,"version":"4.5.0"},"reference-count":48,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2021,8,9]],"date-time":"2021-08-09T00:00:00Z","timestamp":1628467200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Dairy Research Cluster Initiative II"},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Resilient Dairy Genome Project"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>\n                    The aim of this study was to determine the association between concentrations of progesterone\n                    <jats:bold>(P4)<\/jats:bold>\n                    during previous the estrous cycle with the intensity of spontaneous or estrogen-induced estrous expression and pregnancy per artificial insemination\n                    <jats:bold>(P\/AI)<\/jats:bold>\n                    . A total of 1,953 AI events from lactating Holstein cows were used, consisting of 1,289 timed AI events from experiment 1\n                    <jats:bold>(Exp. 1)<\/jats:bold>\n                    and 664 AI events from experiment 2\n                    <jats:bold>(Exp. 2)<\/jats:bold>\n                    . In Exp. 1, cows were bred after a timed AI protocol based on estradiol and P4. In Exp. 2 animals were bred upon spontaneous estrus detection. In both experiments cows were continuously monitored by an automated activity monitor\n                    <jats:bold>(AAM)<\/jats:bold>\n                    , in Exp.1 a relative increase of activity was calculated (i.e., percentage of increase activity at estrus compared to cow\u2019s baseline activity) and in Exp.2, activity data from each cow were computed into an index value that ranged from 0 to 100. In Exp.2 duration (hours) of estrus were calculated and defined as the total time above the threshold (35 index). The intensity of estrous expression was determined for each event and classified as either high or low intensity using the median of each experiment. Blood samples were collected for P4 analysis in Exp. 1 at \u20134 d, 0 d, and 7 d relative to timed AI, and in Exp. 2 immediately following estrus (0 d), 7 d, 14 d, and 21 d post-AI. Concentration of P4 was classified as greater or lower according to the median value in each experiment. Cows with lower concentrations of P4 at AI had greater estrous expression in Exp. 1 (363.6 \u00b1 5.2 vs. 275.9 \u00b1 8.0% relative increase) and Exp. 2 (76.7 \u00b1 1.9 vs. 67.4 \u00b1 4.7 index; and 12.5 \u00b1 0.5 vs. 9.3 \u00b1 1.8 hours). Cows with a greater intensity of estrous expression at timed AI had greater concentrations of P4 at \u20134 d than cows with lower intensity estrus or no estrous expression (4.6 \u00b1 0.2 vs. 3.6 \u00b1 0.2 vs. 3.7 \u00b1 0.2 ng\/mL). Cows with greater concentrations of P4 at \u20134 d had greater P\/AI (32.8 \u00b1 4.4 vs. 22.4 \u00b1 4.5%), whereas cows with lesser concentrations of P4 at d0 for either timed AI (35.2 \u00b1 3.4 vs. 19.6 \u00b1 4.6%) or spontaneous estrus (31.8 \u00b1 2.8 vs. 23.4 \u00b1 3.2%) had greater P\/AI. Cows with greater concentrations of P4 7 d post-timed AI had greater P\/AI compared with cows that had lower concentration of P4 (39.1 \u00b1 2.9 vs. 24.7 \u00b1 2.6%). Similarly, cows that had lower concentrations of P4 at 7 d, 14 d and 21 d post-spontaneous estrus tended to have lower P\/AI when compared with cows with greater concentrations of P4. Overall, concentrations of P4 prior to and at AI were associated with greater estrous intensity and P\/AI at both spontaneous and timed AI events.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0248453","type":"journal-article","created":{"date-parts":[[2021,8,9]],"date-time":"2021-08-09T13:24:03Z","timestamp":1628515443000},"page":"e0248453","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":26,"title":["Plasma concentrations of progesterone in the preceding estrous cycle are associated with the intensity of estrus and fertility of Holstein cows"],"prefix":"10.1371","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9696-8478","authenticated-orcid":true,"given":"Augusto M. 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