{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T03:01:20Z","timestamp":1787022080713,"version":"build-2736575974"},"reference-count":45,"publisher":"Public Library of Science (PLoS)","issue":"3","license":[{"start":{"date-parts":[[2021,3,15]],"date-time":"2021-03-15T00:00:00Z","timestamp":1615766400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005076","name":"Agricultural Research Development Agency","doi-asserted-by":"crossref","award":["CRP6205030570"],"award-info":[{"award-number":["CRP6205030570"]}],"id":[{"id":"10.13039\/501100005076","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Ratchadapisek Sompoch Endowment fund"},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Overseas Research Experience Scholarship, Chulalongkorn University"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>\n                    The emergence and dissemination of extended-spectrum \u03b2-lactamase (ESBL)-producing\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    is a global health issue. Food-producing animals, including pigs, are significant reservoirs of antimicrobial resistance (AMR), which can be transmitted to humans. Thus, the rapid detection of ESBLs is required for efficient epidemiological control and treatment. In this study, multiplex recombinase polymerase amplification (RPA) combined with a single-stranded tag hybridization chromatographic printed-array strip (STH-PAS), as a lateral flow strip assay (LFA), was established for the rapid and simultaneous detection of multiple\n                    <jats:italic>bla<\/jats:italic>\n                    genes in a single reaction. Visible blue lines, indicating the presence of the\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>CTX-M<\/jats:sub>\n                    ,\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>SHV<\/jats:sub>\n                    , and\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>OXA<\/jats:sub>\n                    genes, were observed within 10 min by the naked eye. The limit of detection of all three genes was 2.5 ng\/25 \u03bcL, and no cross-reactivity with seven commensal aerobic bacteria was observed. A total of 93.9% (92\/98) and 96% (48\/50) of the\n                    <jats:italic>E<\/jats:italic>\n                    .\n                    <jats:italic>coli<\/jats:italic>\n                    isolates from pork meat and fecal samples, respectively, expressed an ESBL-producing phenotype. Nucleotide sequencing of the PCR amplicons showed that\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>CTX-M<\/jats:sub>\n                    was the most prevalent type (91.3\u201395.83%), of which the main form was\n                    <jats:italic>bla<\/jats:italic>\n                    <jats:sub>CTX-M-55<\/jats:sub>\n                    . The sensitivity and specificity of the RPA-LFA were 99.2% and 100%, respectively, and were in almost perfect agreement (\u03ba = 0.949\u20131.000) with the results from PCR sequencing. Thus, the RPA-LFA is a promising tool for rapid and equipment-free ESBL detection and may facilitate clinical diagnosis in human and veterinary medicine, as well as AMR monitoring and surveillance.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0248536","type":"journal-article","created":{"date-parts":[[2021,3,15]],"date-time":"2021-03-15T13:25:02Z","timestamp":1615814702000},"page":"e0248536","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":41,"title":["Rapid detection of extended spectrum \u03b2-lactamase producing Escherichia coli isolated from fresh pork meat and pig cecum samples using multiplex recombinase polymerase amplification and lateral flow strip analysis"],"prefix":"10.1371","volume":"16","author":[{"given":"Sitthichai","family":"Kanokudom","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thachaporn","family":"Assawakongkarat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukihiro","family":"Akeda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Panan","family":"Ratthawongjirakul","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rungtip","family":"Chuanchuen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0717-5466","authenticated-orcid":true,"given":"Nuntaree","family":"Chaichanawongsaroj","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2021,3,15]]},"reference":[{"issue":"1","key":"pone.0248536.ref001","first-page":"4","volume":"127","author":"TF Landers","year":"2012","journal-title":"A Review of Antibiotic Use in Food Animals: Perspective, Policy, and Potential"},{"key":"pone.0248536.ref002","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1186\/1746-6148-8-21","article-title":"Occurrence and characteristics of extended-spectrum \u03b2-lactamase (ESBL) producing Enterobacteriaceae in food producing animals, minced meat and raw milk","volume":"8","author":"N Geser","year":"2012","journal-title":"BMC veterinary research"},{"issue":"11","key":"pone.0248536.ref003","doi-asserted-by":"crossref","first-page":"3612","DOI":"10.1007\/s13197-017-2820-4","article-title":"Antimicrobial resistance in diarrheagenic Escherichia coli from ready-to-eat foods","volume":"54","author":"CM Lima","year":"2017","journal-title":"J Food Sci Tech Mys"},{"issue":"4","key":"pone.0248536.ref004","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1590\/S0036-46652014000400012","article-title":"Antimicrobial drug resistance in strains of Escherichia coli isolated from food sources","volume":"56","author":"MU Rasheed","year":"2014","journal-title":"Rev Inst Med Trop Sao Paulo"},{"issue":"10","key":"pone.0248536.ref005","doi-asserted-by":"crossref","first-page":"917","DOI":"10.1016\/j.cmi.2015.05.032","article-title":"Carriage of extended-spectrum \u03b2-lactamases in pig farmers is associated with occurrence in pigs","volume":"21","author":"W Dohmen","year":"2015","journal-title":"Clin Microbiol Infect"},{"issue":"3","key":"pone.0248536.ref006","doi-asserted-by":"crossref","first-page":"e0174094","DOI":"10.1371\/journal.pone.0174094","article-title":"Risk factors for ESBL-producing Escherichia coli on pig farms: A longitudinal study in the context of reduced use of antimicrobials","volume":"12","author":"W Dohmen","year":"2017","journal-title":"PLoS One"},{"key":"pone.0248536.ref007","unstructured":"Tacconelli E, Magrini N. 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