{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T14:07:42Z","timestamp":1740146862464,"version":"3.37.3"},"reference-count":51,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2021,4,1]],"date-time":"2021-04-01T00:00:00Z","timestamp":1617235200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012309","name":"Royal Golden Jubilee (RGJ) Ph.D. Programme","doi-asserted-by":"publisher","award":["grant number PHD\/0108\/2557"],"award-info":[{"award-number":["grant number PHD\/0108\/2557"]}],"id":[{"id":"10.13039\/501100012309","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Center of Excellence on Biodiversity (BDC) Office of Higher Education Commission","award":["BDC-PG3-163005"],"award-info":[{"award-number":["BDC-PG3-163005"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Q fever is one of the most important zoonotic diseases caused by the obligate intracellular bacteria,<jats:italic>Coxiella burnetii<\/jats:italic>. This bacterial infection has been frequently reported in both humans and animals, especially ruminants. Ticks are important ectoparasite and serve as reservoir hosts of<jats:italic>Coxiella<\/jats:italic>-like endosymbionts (CLEs). In this study, we have attempted to express chaperone-coding genes from CLEs of<jats:italic>Rhipicephalus annulatus<\/jats:italic>ticks collected fromcow path. The partial DnaK coding sequence has been amplified and expressed by<jats:italic>Escherichia coli<\/jats:italic>. Amino acid sequences have been analyzed by MS-MS spectrometry and the UniProt database. Despites nucleotide sequences indicating high nucleotide variation and diversity, many nucleotide substitutions are synonymous. In addition, amino acid substitutions compensate for the physicochemical properties of the original amino acids. Immune Epitope Database and Analysis Resource (IEDB-AR) was employed to indicate the antigenicity of the partial DnaK protein and predict the epitopes of B-and T-cells. Interestingly, some predicted HLA-A and B alleles of the MHC-I and HLA-DR alleles belonging to MHC-II were similar to T-cell responses to<jats:italic>C<\/jats:italic>.<jats:italic>burnetii<\/jats:italic>in Q fever patients. Therefore, the partial DnaK protein of CLE from<jats:italic>R<\/jats:italic>.<jats:italic>annulatus<\/jats:italic>could be considered a vaccine candidate and immunogenic marker with future prospects.<\/jats:p>","DOI":"10.1371\/journal.pone.0249354","type":"journal-article","created":{"date-parts":[[2021,4,1]],"date-time":"2021-04-01T18:14:28Z","timestamp":1617300868000},"page":"e0249354","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":1,"title":["Partial DnaK protein expression from Coxiella-like endosymbiont of Rhipicephalus annulatus tick"],"prefix":"10.1371","volume":"16","author":[{"given":"Pornpiroon","family":"Nooroong","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wachareeporn","family":"Trinachartvanit","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Visut","family":"Baimai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Panat","family":"Anuracpreeda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4252-9968","authenticated-orcid":true,"given":"Arunee","family":"Ahantarig","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2021,4,1]]},"reference":[{"issue":"8","key":"pone.0249354.ref001","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1038\/nrmicro3049","article-title":"Molecular 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Thailand among livestock in two districts at the Thai-Cambodian border, Sa Kaeo province","author":"S Colombe","year":"2018","journal-title":"One Health"},{"issue":"5","key":"pone.0249354.ref012","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0216652","article-title":"Seropositivities against brucellosis, coxiellosis, and toxoplasmosis and associated factors in pregnant women with adverse pregnancy outcomes: A cross-sectional study","volume":"14","author":"K Kledmanee","year":"2019","journal-title":"PLoS One"},{"issue":"3","key":"pone.0249354.ref013","doi-asserted-by":"crossref","first-page":"473","DOI":"10.3201\/eid2003.131059","article-title":"Infective endocarditis in northeastern Thailand","volume":"20","author":"G Watt","year":"2014","journal-title":"Emerg Infect Dis"},{"issue":"1","key":"pone.0249354.ref014","doi-asserted-by":"crossref","DOI":"10.4081\/idr.2012.3910","article-title":"The first reported cases of Q fever endocarditis in Thailand","volume":"4","author":"O Pachirat","year":"2012","journal-title":"Infect Dis Rep"},{"issue":"1","key":"pone.0249354.ref015","doi-asserted-by":"crossref","first-page":"252","DOI":"10.4269\/ajtmh.17-0413","article-title":"Acute Q fever case detection among acute febrile illness patients, Thailand, 2002\u20132005","volume":"98","author":"AL Greiner","year":"2018","journal-title":"Am J Trop Med Hyg"},{"issue":"1","key":"pone.0249354.ref016","doi-asserted-by":"crossref","first-page":"670","DOI":"10.1186\/s13071-018-3259-9","article-title":"Coxiella-like bacteria in fowl ticks from Thailand","volume":"11","author":"W Trinachartvanit","year":"2018","journal-title":"Parasit Vectors"},{"issue":"5","key":"pone.0249354.ref017","doi-asserted-by":"crossref","first-page":"678","DOI":"10.1016\/j.ttbdis.2016.02.015","article-title":"Molecular detection of Rickettsia, Anaplasma, Coxiella and Francisella bacteria in ticks collected from Artiodactyla in Thailand","volume":"7","author":"C Sumrandee","year":"2016","journal-title":"Ticks Tick Borne Dis"},{"issue":"4","key":"pone.0249354.ref018","doi-asserted-by":"crossref","first-page":"963","DOI":"10.1016\/j.ttbdis.2018.03.027","article-title":"Phylogenetic studies of bacteria (Rickettsia, Coxiella, and Anaplasma) in Amblyomma and Dermacentor ticks in Thailand and their co-infection","volume":"9","author":"P Nooroong","year":"2018","journal-title":"Ticks Tick Borne Dis"},{"issue":"1","key":"pone.0249354.ref019","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.ttbdis.2014.09.005","article-title":"Detection of Coxiella-like endosymbiont in Haemaphysalis tick in Thailand","volume":"6","author":"W Arthan","year":"2015","journal-title":"Ticks Tick Borne Dis"},{"key":"pone.0249354.ref020","first-page":"643","article-title":"Co-infection with Coxiella-like bacteria and Babesia in goat ticks from southern Thailand","volume":"50","author":"A Ahantarig","year":"2019","journal-title":"The Southeast Asian journal of tropical medicine and public health"},{"issue":"3","key":"pone.0249354.ref021","doi-asserted-by":"crossref","first-page":"377","DOI":"10.4149\/av_2017_320","article-title":"Coxiella burnetii immunogenic proteins as a basis for new Q fever diagnostic and vaccine development","volume":"61","author":"C Gerlach","year":"2017","journal-title":"Acta Virol"},{"issue":"2","key":"pone.0249354.ref022","doi-asserted-by":"crossref","first-page":"642","DOI":"10.1128\/IAI.01141-08","article-title":"Comparative genomics reveal extensive transposon-mediated genomic plasticity and diversity among potential effector proteins within the genus Coxiella","volume":"77","author":"PA Beare","year":"2009","journal-title":"Infection and immunity"},{"issue":"5","key":"pone.0249354.ref023","doi-asserted-by":"crossref","first-page":"e1004892","DOI":"10.1371\/journal.ppat.1004892","article-title":"The recent evolution of a maternally-inherited endosymbiont of ticks led to the emergence of the Q fever pathogen, Coxiella burnetii","volume":"11","author":"O Duron","year":"2015","journal-title":"PLoS Pathogens"},{"issue":"11","key":"pone.0249354.ref024","doi-asserted-by":"crossref","first-page":"2905","DOI":"10.1111\/mec.14094","article-title":"Evolutionary changes in symbiont community structure in ticks","volume":"26","author":"O Duron","year":"2017","journal-title":"Mol Eco"},{"issue":"5","key":"pone.0249354.ref025","doi-asserted-by":"crossref","first-page":"1751","DOI":"10.1111\/1462-2920.14104","article-title":"Comparison of closely related, uncultivated Coxiella tick endosymbiont population genomes reveals clues about the mechanisms of symbiosis","volume":"20","author":"D Tsementzi","year":"2018","journal-title":"Environ Microbiol"},{"issue":"1","key":"pone.0249354.ref026","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1128\/AEM.02009-06","article-title":"Highly prevalent Coxiella sp. bacterium in the tick vector Amblyomma americanum","volume":"73","author":"A Jasinskas","year":"2007","journal-title":"Appl Environ Microbiol"},{"issue":"4","key":"pone.0249354.ref027","doi-asserted-by":"crossref","first-page":"748","DOI":"10.1016\/S0741-5214(99)70329-0","article-title":"Heat shock proteins: A review of the molecular chaperones","volume":"29","author":"D Whitley","year":"1999","journal-title":"J Vasc Surg"},{"key":"pone.0249354.ref028","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1007\/978-94-007-4315-1_6","article-title":"Proteome of Coxiella burnetii","volume":"984","author":"R Ihnatko","year":"2012","journal-title":"Adv Exp Med Biol"},{"issue":"307","key":"pone.0249354.ref029","article-title":"Anaplasma phagocytophilum MSP4 and HSP70 proteins are involved in interactions with host cells during pathogen infection","volume":"7","author":"M Contreras","year":"2017","journal-title":"Front Cell Infect 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FigTree version 1.3.1 [computer program] http:\/\/tree.bio.ed.ac.uk."},{"key":"pone.0249354.ref032","first-page":"60","article-title":"BioEdit: An important software for molecular biology","volume":"2","author":"A Alzohairy","year":"2011","journal-title":"GERF Bulletin of Biosciences"},{"issue":"11","key":"pone.0249354.ref033","doi-asserted-by":"crossref","first-page":"1451","DOI":"10.1093\/bioinformatics\/btp187","article-title":"DnaSP v5: a software for comprehensive analysis of DNA polymorphism data","volume":"25","author":"P Librado","year":"2009","journal-title":"Bioinformatics"},{"issue":"9","key":"pone.0249354.ref034","doi-asserted-by":"crossref","first-page":"1110","DOI":"10.1111\/2041-210X.12410","article-title":"popart: full-feature software for haplotype network construction","volume":"6","author":"JW Leigh","year":"2015","journal-title":"Methods Ecol Evol"},{"issue":"1","key":"pone.0249354.ref035","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1038\/nmeth.3213","article-title":"The I-TASSER suite: protein structure and function prediction","volume":"12","author":"J Yang","year":"2015","journal-title":"Nat Methods"},{"key":"pone.0249354.ref036","unstructured":"Discovery Studio Modeling Environment, Release 4.5. 2015. BIOVIA, Dassault Syst\u00e8mes, San Diego."},{"key":"pone.0249354.ref037","doi-asserted-by":"crossref","first-page":"W513","DOI":"10.1093\/nar\/gkn254","article-title":"Immune epitope database analysis resource (IEDB-AR)","volume":"36","author":"Q Zhang","year":"2008","journal-title":"Nucleic acids research"},{"issue":"3","key":"pone.0249354.ref038","doi-asserted-by":"crossref","first-page":"831","DOI":"10.1093\/gbe\/evv016","article-title":"A Coxiella-Like Endosymbiont is a potential vitamin source for the lone star tick","volume":"7","author":"TA Smith","year":"2015","journal-title":"Genome Biol Evol"},{"issue":"17","key":"pone.0249354.ref039","article-title":"The IS1111 insertion sequence used for detection of Coxiella burnetii is widespread in Coxiella-like endosymbionts of ticks","volume":"362","author":"O Duron","year":"2015","journal-title":"FEMS Microbiol Lett"},{"issue":"4","key":"pone.0249354.ref040","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1093\/molbev\/msz003","article-title":"Physicochemical amino acid properties better describe substitution rates in large populations","volume":"36","author":"CC Weber","year":"2019","journal-title":"Mol Biol Evol"},{"issue":"1","key":"pone.0249354.ref041","first-page":"19","article-title":"Epidemiology of Q fever in ruminants in the north-east and the north region of Thailand, 2012 to 20132018","volume":"28","author":"P Opaschaitat","year":"2018","journal-title":"KKU Veterinary Journal"},{"issue":"10","key":"pone.0249354.ref042","doi-asserted-by":"crossref","first-page":"818","DOI":"10.14202\/vetworld.2013.818-822","article-title":"Genetic characterization of Coxiella burnetii in Amblyomma varigatum ticks from North-central Nigeria: public health importance","volume":"6","author":"NI Ogo","year":"2013","journal-title":"Veterinary World"},{"issue":"12","key":"pone.0249354.ref043","doi-asserted-by":"crossref","first-page":"744","DOI":"10.1089\/vbz.2016.1959","article-title":"Coxiella detection in ticks from wildlife and livestock in Malaysia","volume":"16","author":"JJ Khoo","year":"2016","journal-title":"Vector Borne Zoonotic Dis"},{"issue":"2","key":"pone.0249354.ref044","doi-asserted-by":"crossref","first-page":"54","DOI":"10.3390\/tropicalmed5020054","article-title":"Molecular detection of Rickettsia spp. and Coxiella burnetii in cattle, water buffalo, and Rhipicephalus (Boophilus) microplus ticks in Luzon Island of the Philippines","volume":"5","author":"RL Galay","year":"2020","journal-title":"Tropical Medicine and Infectious Disease"},{"issue":"7","key":"pone.0249354.ref045","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1186\/gm266","article-title":"Proteomics paves the way for Q fever diagnostics","volume":"3","author":"M Kowalczewska","year":"2011","journal-title":"Genome Med"},{"issue":"7","key":"pone.0249354.ref046","doi-asserted-by":"crossref","first-page":"1150","DOI":"10.1016\/j.jprot.2011.04.016","article-title":"A proteomic approach to investigate the differential antigenic profile of two Coxiella burnetii strains","volume":"74","author":"A Papadioti","year":"2011","journal-title":"J Proteomics"},{"key":"pone.0249354.ref047","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1186\/1471-2180-12-35","article-title":"Potential serodiagnostic markers for Q fever identified in Coxiella burnetii by immunoproteomic and protein microarray approaches","volume":"12","author":"X Xiong","year":"2012","journal-title":"BMC Microbiol"},{"issue":"10","key":"pone.0249354.ref048","doi-asserted-by":"crossref","first-page":"e46688","DOI":"10.1371\/journal.pone.0046688","article-title":"Predicting the functional effect of amino acid substitutions and indels","volume":"7","author":"Y Choi","year":"2012","journal-title":"PLoS One"},{"key":"pone.0249354.ref049","first-page":"91","volume-title":"Coxiella burnetii: recent advances and new perspectives in research of the Q fever bacterium. 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