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The study was performed using eyeball morphometry, analysis of the bony orbit including its morphometry, macroscopic study, morphometry, histological examination of the eye tunics and chosen accessory organs of the eye and histochemical analysis. The orbit was funnel shaped and was open-type. There was a single ethmoid opening for the ethmoid nerve on the orbital lamina. The pupil was round, while the ciliary body occupied a relatively wide zone. The iris was brown and retina had a pigmented area. The cellular<jats:italic>tapetum lucidum<\/jats:italic>was semi-circular and milky and was composed of 14\u201317 layers of tapetal cells arranged in a bricklike structure. In the lower eyelid, there was a single conjunctival lymph nodule aggregate. One or two additional large conjunctval folds were observed within the posterior surface of the upper eyelids. The superficial gland of the third eyelid had a serous nature. The third eyelid was T-shaped and was composed of hyaline tissue. Two to three conjunctival lymph nodul aggregates were present within the bulbar conjunctiva of the third eyelid. The lacrimal gland produced a sero-mucous secretion. A detailed anatomic analysis of the eye area in the captive South African Painted Dogs females showed the similarities (especially in the histological examination of the eyetunics and orbital glands) as well as the differences between the Painted dog and the other representatives of<jats:italic>Canidae<\/jats:italic>. The differences included the shape and size od the orbita with comparison to the domestic dog. Such differences in the orbit measurements are most likely associated with the skull type, which are defined in relation to domestic dogs. The presented results significantly expand the existing knowledge on comparative anatomy in the orbit, eye and chosen accessory organs in wild<jats:italic>Canidae<\/jats:italic>.<\/jats:p>","DOI":"10.1371\/journal.pone.0249368","type":"journal-article","created":{"date-parts":[[2021,4,19]],"date-time":"2021-04-19T18:58:22Z","timestamp":1618858702000},"page":"e0249368","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":10,"title":["Anatomical and morphometric evaluation of the orbit, eye tunics, eyelids and orbital glands of the captive females of the South African painted dog (Lycaon pictus pictus Temminck, 1820) (Caniformia: Canidae)"],"prefix":"10.1371","volume":"16","author":[{"given":"Wojciech","family":"Paszta","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joanna E.","family":"Kle\u0107kowska-Nawrot","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4412-1966","authenticated-orcid":true,"given":"Karolina","family":"Go\u017adziewska-Har\u0142ajczuk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2021,4,19]]},"reference":[{"key":"pone.0249368.ref001","unstructured":"Woodroffe R, Sillero-Zubiri C. Lycaon pictus. The IUCN Red List of Threatened Species International Union for Conservation of Nature. 2012; e.T12436A16711116."},{"key":"pone.0249368.ref002","doi-asserted-by":"crossref","DOI":"10.1525\/9780520352377","volume-title":"The Behavior Guide to African Mammals","author":"R Estes","year":"2012"},{"key":"pone.0249368.ref003","doi-asserted-by":"crossref","first-page":"1122","DOI":"10.1644\/05-MAMM-A-304R2.1","article-title":"Prey preferences of the African wild dog Lycaon pictus (Canidae: Carnivora): ecological requirements for conservation","volume":"87","author":"MW Hayward","year":"2006","journal-title":"J Mammal"},{"key":"pone.0249368.ref004","first-page":"56","volume-title":"The Encyclopedia of Mammals","author":"JR Malcolm","year":"1999","edition":"2"},{"key":"pone.0249368.ref005","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1017\/S0952836903004576","article-title":"Prey selection by African wild dogs (Lycaon pictus) in southern Zimbabwe","volume":"262","author":"A Pole","year":"2004","journal-title":"J Zool"},{"key":"pone.0249368.ref006","unstructured":"Smithers RHN. The Mammals of Southern African Subregion. University of Pretoria, Pretoria Republic of South Africa. 1983."},{"key":"pone.0249368.ref007","first-page":"69","article-title":"Likaon\u2014gin\u0105cy drapie\u017cnik Afryki","volume":"1\u20132","author":"A Z\u0142amal","year":"2000","journal-title":"Kosmos. 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Global Information Serving Conservation."},{"key":"pone.0249368.ref014","doi-asserted-by":"crossref","first-page":"386","DOI":"10.1111\/j.1463-5224.2008.00663.x","article-title":"The capybara eye: clinical testes, anatomic and biometric features","volume":"11","author":"F Montiani-Ferreira","year":"2008","journal-title":"Vet Ophthalmol"},{"key":"pone.0249368.ref015","volume-title":"A Taxonomic and Geographic Reference","author":"DE Wilson","year":"2005"},{"key":"pone.0249368.ref016","doi-asserted-by":"crossref","first-page":"e36097","DOI":"10.1371\/journal.pone.0036097","article-title":"Eye Size at Birth in Prosimian Primates: Life History Correlates and Growth Patterns","volume":"7","author":"JR Cummings","year":"2012","journal-title":"Plos ONE"},{"key":"pone.0249368.ref017","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1111\/j.1439-0264.2007.00796.x","article-title":"Eye morphology in some wild rodents","volume":"37","author":"S Lluch","year":"2008","journal-title":"Anat Histol Embryol"},{"key":"pone.0249368.ref018","doi-asserted-by":"crossref","first-page":"1095","DOI":"10.1002\/ar.a.20115","article-title":"Comparative morphology of the eye in primates","volume":"281A","author":"EC Kirk","year":"2004","journal-title":"Anat Rec"},{"key":"pone.0249368.ref019","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.jhevol.2006.02.004","article-title":"Effects of activity pattern of the eye size and orbital aperture size of primates","volume":"51","author":"EC Kirk","year":"2006","journal-title":"J Human Evol"},{"key":"pone.0249368.ref020","volume-title":"Philadelphia London Toronto Montreal Sydney Tokyo","author":"HE Evans","year":"1994","edition":"3"},{"key":"pone.0249368.ref021","doi-asserted-by":"crossref","first-page":"e0224245","DOI":"10.1371\/journal.pone.0224245","article-title":"Anatomical, histological and computed tomography comparision of the eye and adnexa of crab-eating fox (Cerdocyon thous) to domestic dogs","volume":"14","author":"NL Lantyer-Araujo","year":"2019","journal-title":"Plos ONE"},{"key":"pone.0249368.ref022","doi-asserted-by":"crossref","first-page":"449","DOI":"10.4067\/S0717-95022006000400025","article-title":"Morphological and craniometrical studies on the skull of kagani goat (Capra hircus) of Jammu region","volume":"24","author":"K Sarma","year":"2006","journal-title":"Int J Morphol"},{"key":"pone.0249368.ref023","first-page":"72","volume-title":"Pathological technique","author":"FB Mallory","year":"1938"},{"key":"pone.0249368.ref024","first-page":"289","article-title":"Demonstration of all connective tissue elements in a single section","volume":"60","author":"HZ Movat","year":"1955","journal-title":"AMA Archiv Pathol"},{"key":"pone.0249368.ref025","volume-title":"Technika histologiczna","author":"NC Burck","year":"1975"},{"key":"pone.0249368.ref026","first-page":"151","volume-title":"Histopathologic methods and color atlas of special stains and tissue artifacts","author":"LG Lee","year":"1992"},{"key":"pone.0249368.ref027","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1016\/j.aanat.2014.06.005","article-title":"Age-related alterations in the lacrimal gland of the adult albino rat: A light and electron microscopic study","volume":"196","author":"AB El-Fadaly","year":"2014","journal-title":"Ann Anat"},{"key":"pone.0249368.ref028","first-page":"78","volume-title":"Series on Methods and Achievements in Experimental Pathology","author":"SC Spicer","year":"1967"},{"key":"pone.0249368.ref029","first-page":"173","volume-title":"Theory and Practice of Histological Techniques","author":"JD Bancroft","year":"2008","edition":"6"},{"key":"pone.0249368.ref030","first-page":"126","volume-title":"Histotechnology a self-instructional text","author":"F Carson","year":"1990","edition":"1"},{"key":"pone.0249368.ref031","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1620\/tjem.145.251","article-title":"An application of the high-iron diamine staining for detection of sulfated glycoproteins (glycopeptides) in electrophoresis on cellulose acetate membrane","volume":"145","author":"H Munakata","year":"1985","journal-title":"Tohoku J Exp Med"},{"key":"pone.0249368.ref032","first-page":"164","volume-title":"Theory and practice histotechnology","author":"DC Sheehan","year":"1980","edition":"2"},{"key":"pone.0249368.ref033","unstructured":"Nomina Anatomica Veterinaria. Sixth edition (revised version). Published by the Editoral Committee. Hanover (Germany), Ghent (Belgium), Columbia, MO (U.S.A.), Rio de Janeiro (Brazil). 2017."},{"key":"pone.0249368.ref034","unstructured":"Nomina Histologica Veterinaria. Submitted by the International Committee on Veterinary Histological Nomenclature (ICVHN) to the World Association Veterinary Anatomists. 2017."},{"key":"pone.0249368.ref035","volume-title":"Lehrbuch der Anatomie der Haustiere","author":"R Nickel","year":"2004"},{"key":"pone.0249368.ref036","doi-asserted-by":"crossref","first-page":"249","DOI":"10.4159\/harvard.9780674431263.c12","volume-title":"Evolution of African Mammals","author":"RJG Savage","year":"1978"},{"key":"pone.0249368.ref037","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1016\/0168-9525(93)90122-X","article-title":"Molecular evolution of the dog family","volume":"9","author":"RK Wayne","year":"1993","journal-title":"Trends in Genetics"},{"key":"pone.0249368.ref038","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1111\/jzo.1982.198.4.443","article-title":"Reproductive and social behavior of the dhole (Cuon alpinus) (Canidae)","volume":"198","author":"AJT Johnsingh","year":"1982","journal-title":"J Zool"},{"key":"pone.0249368.ref039","first-page":"934","article-title":"Do dholes (Cuon aplinus) live in pascks in response to competention or predation by large cats?","volume":"69","author":"A Venkataraman","year":"1995","journal-title":"Curr Sci"},{"key":"pone.0249368.ref040","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1159\/000124409","article-title":"Evolution of the canid brain","volume":"7","author":"L Radinsky","year":"1973","journal-title":"Brain Beh Evol"},{"key":"pone.0249368.ref041","doi-asserted-by":"crossref","first-page":"460","DOI":"10.1111\/vop.12741","article-title":"Ophthalmic contribution to assessing eyes of the two neotropical canids: Cerdocyon thous and Chrysocyon brachyurus","volume":"23","author":"CM Carvalho","year":"2020","journal-title":"Vet Ophthalmol"},{"key":"pone.0249368.ref042","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1638\/2018-0129","article-title":"The eye of crab-eating fox (Cerdocyon thous): anatomical characteristics 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Ophthalmol"},{"key":"pone.0249368.ref049","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1111\/j.1439-0264.1998.tb00164.x","article-title":"Morphomterical features of the corneal epithelium in mammals","volume":"27","author":"MD Merindano","year":"1998","journal-title":"Anat Histol Embryol"},{"key":"pone.0249368.ref050","first-page":"200","article-title":"A comparative study of the tapetum, retina, and skull of the ferret, dog, and cat","volume":"35","author":"GY Wen","year":"1985","journal-title":"Lab Anim Sci"},{"key":"pone.0249368.ref051","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1111\/j.1463-5224.2004.00318.x","article-title":"Comparative morphology of the tapetum lucidum (among selected species)","volume":"7","author":"FJ Ollivier","year":"2004","journal-title":"Vet Ophthalmol"},{"key":"pone.0249368.ref052","doi-asserted-by":"crossref","first-page":"1099","DOI":"10.1292\/jvms.14-0132","article-title":"Macroscopic and histological vartiations in the cellular tapetum in dogs","volume":"76","author":"Y Yamaue","year":"2014","journal-title":"J Vet Med Sci"},{"key":"pone.0249368.ref053","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1007\/BF02764312","article-title":"Histological study of choroidal melanocytes in animals with tapetum lucidum cellulosum","volume":"228","author":"T Chijiiwa","year":"1990","journal-title":"Graefe\u2019s Archiv Clinic Exp Ophthalmol"},{"key":"pone.0249368.ref054","first-page":"157","article-title":"Fine structure of the canine tapetum lucidum","volume":"136","author":"TP Lesiuk","year":"1983","journal-title":"J Anat"},{"key":"pone.0249368.ref055","first-page":"153","article-title":"Skull typology and morphometrics of the Nigerian local dog (Canis lupus familiaris)","volume":"32","author":"OO Igado","year":"2017","journal-title":"Niger J Physiol Sci"},{"key":"pone.0249368.ref056","doi-asserted-by":"crossref","first-page":"997","DOI":"10.1111\/1365-2656.12076","article-title":"Bergmann\u2019s 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Knoest","year":"2015"},{"key":"pone.0249368.ref060","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1046\/j.1439-0264.2001.00292.x","article-title":"Skull typology of adult male Kangal dogs","volume":"30","author":"V Onar","year":"2001","journal-title":"Anat Histol Embryol"},{"key":"pone.0249368.ref061","volume-title":"Peabody Museum Bulletin","author":"A Von den Driesch","year":"1976"},{"key":"pone.0249368.ref062","doi-asserted-by":"crossref","first-page":"640","DOI":"10.4067\/S0717-95022020000300640","article-title":"Craniometry in Cerdocyon thous (Carnivora, Canidae)","volume":"38","author":"AM Neto","year":"2020","journal-title":"Int J Morphol"},{"key":"pone.0249368.ref063","doi-asserted-by":"crossref","first-page":"1","DOI":"10.2307\/3503974","article-title":"Cerdocyon thous","volume":"186","author":"A Berta","year":"1982","journal-title":"Mammalian Species"},{"key":"pone.0249368.ref064","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1644\/848.1","article-title":"Lycalopex sechurae (Carnivora: Canidae)","volume":"42","author":"ED Cossios","year":"2010","journal-title":"Mamm Spec"},{"key":"pone.0249368.ref065","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1644\/847.1","article-title":"Lycalopex vetulus (Carnivora: Canidae)","volume":"847","author":"JC Daloponte","year":"2009","journal-title":"Mamm Spec"},{"key":"pone.0249368.ref066","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1007\/s11692-015-9362-3","article-title":"Ecogeographical Variation in Skull Shape of South-American Canids: Abiotic or Biotic Processes?","volume":"43","author":"J De Moura Bubadue","year":"2016","journal-title":"Evol Biol"},{"key":"pone.0249368.ref067","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1644\/820.1","article-title":"Lycalopex gymnocercus (Carnivora: Canidae)","volume":"820","author":"M Lucherini","year":"2008","journal-title":"Mamm Spec"},{"key":"pone.0249368.ref068","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1111\/j.1095-8312.2009.01274.x","article-title":"Investigating South American biogeographic history using patterns of skull shape variation on Cerdocyon thous (Mammalia: Canidae)","volume":"98","author":"A Machado F de","year":"2009","journal-title":"Biol J Linnean Soc"},{"key":"pone.0249368.ref069","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1111\/mam.12085","article-title":"Evolutionary ecomorphology of the Falkland Islands wolf Dusicyon austarlis","volume":"47","author":"C Meloro","year":"2017","journal-title":"Mamm Rev"},{"key":"pone.0249368.ref070","first-page":"289","article-title":"Revision of the systematic status of patagonian and pampean gray foxes (Canidae: Lycalopex griseus and L. gymnocercus) using 3d geometric morphometrics","volume":"20","author":"FJ Prevosti","year":"2013","journal-title":"Mastozool Neotrop"},{"key":"pone.0249368.ref071","doi-asserted-by":"crossref","first-page":"704","DOI":"10.1111\/bij.12625","article-title":"Extinctions in near time: new radiocarbon dates point to a very recent disappearance of the South American fox Dusicyon avus (Carnivora: Canidae)","volume":"116","author":"FJ Prevosti","year":"2015","journal-title":"Biol J Linnean Soc"},{"key":"pone.0249368.ref072","first-page":"37693","article-title":"Craniometria am lobos-guara Chrysocyon brachyurus Illiger, 1815 (Carnivira: Canidae)","volume":"18e","author":"ALQ Santos","year":"2017","journal-title":"Cien Anim Bras"},{"key":"pone.0249368.ref073","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1007\/s00435-012-0145-4","article-title":"A quantitative approach of the cranial ontogeny of Lycalopex culpaeus (Carnivora: Canidae)","volume":"131","author":"V Segura","year":"2012","journal-title":"Zoomorphol"},{"issue":"2","key":"pone.0249368.ref074","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1515\/mammalia-2012-0028","article-title":"Skull ontogeny of Lycalopex culpaeus (Carnivora: Canidae): description of the cranial traits and craniofacial sutures","volume":"77","author":"V Segura","year":"2013","journal-title":"Mamm"},{"key":"pone.0249368.ref075","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1111\/jbi.12984","article-title":"Morphological and ecological divergence in South American canids","volume":"44","author":"JP Zurano","year":"2017","journal-title":"J Biogeography"},{"key":"pone.0249368.ref076","first-page":"229","article-title":"Clinical anatomy of the eyelids for small animal practitioners","volume":"85","author":"GM Constantinescu","year":"1998","journal-title":"Wien Tier Monat"},{"key":"pone.0249368.ref077","first-page":"686","volume-title":"Fundamento de ophthalmologia veterinaria","author":"DH Slatter","year":"2005","edition":"3"},{"key":"pone.0249368.ref078","first-page":"1925","article-title":"Density and distribution of canine conjunctival goblet cells","volume":"28","author":"CP Moore","year":"1985","journal-title":"Invest Ophthalmol Vis Scie"},{"key":"pone.0249368.ref079","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/0014-4835(79)90008-3","article-title":"Human ocular mucus. Origins and preliminary characterisation","volume":"29","author":"JC Moore","year":"1979","journal-title":"Exp Eye Res"},{"key":"pone.0249368.ref080","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1111\/j.1463-5224.2004.00325.x","article-title":"The tear film and ocular mucins","volume":"7","author":"HJ Davidson","year":"2004","journal-title":"Vet Ophthalmol"},{"key":"pone.0249368.ref081","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1111\/azo.12223","article-title":"The differences in the eyelids microstructure and the conjunctiva-associated lymphoid tissue between selected ornamental and wild birds as a results of adaptation to their habitat","volume":"99","author":"JE Kle\u0107kowska-Nawrot","year":"2018","journal-title":"Acta Zool"},{"key":"pone.0249368.ref082","doi-asserted-by":"crossref","first-page":"2322","DOI":"10.1167\/iovs.07-0691","article-title":"Local production of secretory IgA in the eye-associated lymphoid tissue (EALT) of the normal human ocular surface","volume":"49","author":"E Knop","year":"2008","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"pone.0249368.ref083","doi-asserted-by":"crossref","first-page":"e82355","DOI":"10.1371\/journal.pone.0082355","article-title":"Development, alteration and real time dynamics of the conjunctiva-associated lymphoid tissue","volume":"8","author":"S Siebelmann","year":"2013","journal-title":"Plos ONE"},{"key":"pone.0249368.ref084","first-page":"32","article-title":"Animal experimental studies of indirect lymphography of the eye, face, and neck regions using Iotasul","volume":"15","author":"BI Wenzel-Hora","year":"1982","journal-title":"Lymphology"},{"key":"pone.0249368.ref085","first-page":"127","article-title":"The conjunctival epithelium of the dogs","volume":"175","author":"T Goller","year":"1993","journal-title":"Ann Anat"},{"key":"pone.0249368.ref086","unstructured":"Cazacu P. Researches concerning the morphology of the nictitating gland in dogs. PhD Thesis. IASI. 2010."},{"key":"pone.0249368.ref087","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1111\/vop.12288","article-title":"Gross anatomy and morphometric evaluation of the canine and third eyelid glands","volume":"19","author":"SA Park","year":"2016","journal-title":"Vet Ophthalmol"},{"key":"pone.0249368.ref088","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1590\/S0103-84782005000200023","article-title":"Canine lacrimal and third eyelid superficial glands macroscopic and morphometric characteristics","volume":"35","author":"VP Cabral","year":"2005","journal-title":"Cien Rur"},{"key":"pone.0249368.ref089","doi-asserted-by":"crossref","first-page":"260","DOI":"10.2460\/ajvr.1990.51.02.260","article-title":"Anatomy of the orbital fasciae and the third eyelid in dogs","volume":"51","author":"GM Constantinescu","year":"1990","journal-title":"Am J Vet Res"},{"key":"pone.0249368.ref090","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1111\/j.1463-5224.2004.04007.x","article-title":"Morphologic changes of the anterior corneal epithelium caused by third eyelid removal in dogs","volume":"7","author":"A Saito","year":"2004","journal-title":"Vet Ophthalmol"},{"key":"pone.0249368.ref091","doi-asserted-by":"crossref","first-page":"106","DOI":"10.21608\/bvmj.2016.31277","article-title":"The structural characterization of the lacrimal gland in the adult dog (Canis familiaris)","volume":"31","author":"NI El-naseery","year":"2016","journal-title":"Benha Vet Med J"},{"key":"pone.0249368.ref092","first-page":"520","article-title":"Anatomical and histological study of the lacrimal gland of the adult male dog (Canis familiaris)","volume":"5","author":"MM Shaker","year":"2016","journal-title":"Glob J Bio-Sci Biotechnol"},{"key":"pone.0249368.ref093","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1186\/1746-6148-10-116","article-title":"Computed tomographic imaging characteristics of the normal canine lacrimal glands","volume":"10","author":"AL Zwingenberger","year":"2014","journal-title":"BMC Vet Res"},{"key":"pone.0249368.ref094","first-page":"1566","article-title":"Normal ultrastructure and histochemical characteristics of the canine lacrimal gland","volume":"49","author":"ChL Martin","year":"1988","journal-title":"Am J Vet Res"},{"key":"pone.0249368.ref095","first-page":"439","volume-title":"The Cornea","author":"DW Lamberts","year":"1994"},{"key":"pone.0249368.ref096","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/S0165-2427(98)00132-9","article-title":"Measurement of IgG, IgM and IgA concentrations in canine serum, saliva, tears and bile","volume":"64","author":"AJ German","year":"1998","journal-title":"Vet Immunol Immunopathol"},{"key":"pone.0249368.ref097","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1007\/978-3-319-20633-2","volume-title":"Endoscopic surgery of the lacrimal drainage system","author":"L Presutti","year":"2016"},{"key":"pone.0249368.ref098","first-page":"464","article-title":"Demonstration of the mucous layer of the tear film by electron microscopy","volume":"26","author":"BA Nichols","year":"1985","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"pone.0249368.ref099","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1007\/s10719-005-3698-3","article-title":"Identification of 9-O-acetyl-N-acetylneuraminic acid in normal canine pre-ocular tear film secreted mucins and its depletion in Keratoconjunctivitis sicca","volume":"22","author":"AP Corfield","year":"2005","journal-title":"Glycoconjugate J"},{"key":"pone.0249368.ref100","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/j.tvjl.2007.08.034","article-title":"Clinical evaluation of pimecrolimus eye drops for treatment of canine keratoconjunctivitis sicca: a comparison with cyclosporine A","volume":"179","author":"R Ofri","year":"2009","journal-title":"Vet J"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0249368","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,28]],"date-time":"2024-08-28T19:16:44Z","timestamp":1724872604000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0249368"}},"subtitle":[],"editor":[{"given":"Carlos E.","family":"Ambr\u00f3sio","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2021,4,19]]},"references-count":100,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2021,4,19]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0249368","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,4,19]]}}}