{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T04:14:32Z","timestamp":1747196072948,"version":"3.40.5"},"reference-count":20,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2021,4,12]],"date-time":"2021-04-12T00:00:00Z","timestamp":1618185600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:sec id=\"sec001\">\n<jats:title>Introduction<\/jats:title>\n<jats:p>The Platelet Function Analyzer-100 (PFA-100) is a point of care instrument that simulates plug formation under high shear flow. The PFA-100 measures the time required to occlude the aperture in a biochemically active cartridge and is expressed in a term of closure time (CT). In Algeria, the reference values used in clinical laboratories are of Western origin. However, ethnic, genetic, dietary environmental, and diet differences between populations may affect reference intervals. We established the reference intervals of PFA-100 closure times in healthy Algerian adults according to the International Federation of Clinical Chemistry method, and we compared them with those of Western and Asian countries.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec002\">\n<jats:title>Material and methods<\/jats:title>\n<jats:p>We enrolled 303 healthy blood donors in the study. 218 subjects met inclusion criteria. We analyzed the blood sample on the PFA-100 for CT with both the collagen epinephrine and collagen ADP cartridges.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec003\">\n<jats:title>Results<\/jats:title>\n<jats:p>The reference intervals of PFA-100 collagen epinephrine CT and PFA-100 collagen ADP CT were 91\u2013207 seconds and 71\u2013144 seconds, respectively. Compared to Western and Asian populations, there were significant differences. The upper limits of CTs were higher for Algerians in this study. Our findings show that many healthy Algerians would be incorrectly identified as having a primary hemostasis abnormality according to the reference intervals of the manufacturer and scientific literature.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec004\">\n<jats:title>Conclusion<\/jats:title>\n<jats:p>This report provides the first reference intervals for PFA-100 CTs in healthy Algerian adults. These results improve the accuracy of diagnosis and patient care in Algeria.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pone.0249402","type":"journal-article","created":{"date-parts":[[2021,4,12]],"date-time":"2021-04-12T17:26:10Z","timestamp":1618248370000},"page":"e0249402","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":3,"title":["Establishment of reference intervals for Platelet Function Analyzer -100 closure time in Algerian adults"],"prefix":"10.1371","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3484-7536","authenticated-orcid":true,"given":"Malika","family":"Belkacemi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yassine","family":"Merad","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamed Amine","family":"Merbouh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2021,4,12]]},"reference":[{"issue":"2","key":"pone.0249402.ref001","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1111\/j.1538-7836.2006.01771.x","article-title":"Platelet function analyzer (PFA)-100 closure time in the evaluation of platelet disorders and platelet function","volume":"4","author":"Platelet Physiology Subcommittee of the Scientific and Standardization Committee of the International Society on Thrombosis and Haemostasis","year":"2006","journal-title":"J Thromb Haemost"},{"issue":"3","key":"pone.0249402.ref002","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1080\/10245330400026097","article-title":"The platelet-function analyzer (PFA-100) for evaluating primary hemostasis","volume":"10","author":"M. Franchini","year":"2005","journal-title":"Hematology"},{"issue":"7","key":"pone.0249402.ref003","doi-asserted-by":"crossref","first-page":"710","DOI":"10.1515\/CCLM.2004.121","article-title":"The IFCC recommendation on estimation of reference intervals. The RefVal program","volume":"42","author":"HE Solberg","year":"2004","journal-title":"Clin Chem Lab Med"},{"key":"pone.0249402.ref004","first-page":"C28","article-title":"Defining, Establishing, and Verifying Reference Intervals in the Clinical Laboratory; Approved Guidelines","author":"J Boyd","year":"2010","journal-title":"CLSI document"},{"issue":"1","key":"pone.0249402.ref005","first-page":"62","article-title":"ABO and rhesus blood groups system in Tlemcen population","volume":"7","author":"S Zaoui","year":"2007","journal-title":"West Algerian. BES"},{"issue":"2","key":"pone.0249402.ref006","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1055\/s-2007-995840","article-title":"PFA-100 system: a new method for assessment of platelet dysfunction","volume":"24","author":"EF Mammen","year":"1998","journal-title":"Semin Thromb Hemost"},{"issue":"1","key":"pone.0249402.ref007","first-page":"93","article-title":"Assessment of primary hemostasis by PFA-100 analysis in a tertiary care center","volume":"84","author":"TL Ortel","year":"2000","journal-title":"Thromb Haemost"},{"issue":"4","key":"pone.0249402.ref008","doi-asserted-by":"crossref","first-page":"898","DOI":"10.1046\/j.1365-2141.1999.01660.x","article-title":"Standardization of the PFA-100\u00ae platelet function test in 105 mmol\/l buffered citrate: effect of gender, smoking, and oral contraceptives","volume":"106","author":"M B\u00f6ck","year":"1999","journal-title":"Br J Haematol"},{"issue":"5","key":"pone.0249402.ref009","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1111\/j.1365-2141.2005.05680.x","article-title":"Variables influencing Platelet Function Analyzer-100 closure times in healthy individuals","volume":"130","author":"H Haubelt","year":"2005","journal-title":"Br J Haematol"},{"key":"pone.0249402.ref010","doi-asserted-by":"crossref","DOI":"10.1515\/acm-2017-0001","article-title":"Variables That Affect Results of PFA-100 in a Group of Healthy Blood Donors in the Slovak Population","volume":"17","author":"L Korinkova","year":"2017","journal-title":"Acta Medica Martiniana"},{"issue":"3","key":"pone.0249402.ref011","first-page":"247","article-title":"Variables that affect platelet function analyzer-100 (PFA-100) closure times and establishment of reference intervals in Korean adults","volume":"38","author":"YU Cho","year":"2008","journal-title":"Ann Clin Lab Sci"},{"issue":"Suppl 4","key":"pone.0249402.ref012","doi-asserted-by":"crossref","first-page":"S11","DOI":"10.1016\/S0049-3848(09)70136-7","article-title":"Effects of race and ethnicity on the incidence of venous thromboembolism","volume":"123","author":"RH White","year":"2009","journal-title":"Thromb Res"},{"issue":"2","key":"pone.0249402.ref013","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1055\/s-0034-1365839","article-title":"The influence of diet and nutrients on platelet function","volume":"40","author":"BJ McEwen","year":"2014","journal-title":"Semin Thromb Hemost"},{"issue":"8","key":"pone.0249402.ref014","doi-asserted-by":"crossref","first-page":"808","DOI":"10.1055\/s-0036-1592333","article-title":"Environmental and genetic risk factors associated with venous thromboembolism","volume":"42","author":"M Crous-Bou","year":"2016","journal-title":"Semin Thromb Hemost"},{"issue":"2","key":"pone.0249402.ref015","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.ejmhg.2015.01.002","article-title":"Comparative frequency and allelic distribution of ABO and Rh (D) blood groups of major tribal communities of southern Bangladesh with general population and their determinants","volume":"16","author":"G. Dewan","year":"2015","journal-title":"Egyptian Journal of Medical Human Genetics"},{"issue":"2","key":"pone.0249402.ref016","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1055\/s-0037-1615707","article-title":"Influence of the ABO blood type on the platelet function analyzer PFA-100","volume":"85","author":"G Lippi","year":"2001","journal-title":"Thromb Haemost"},{"issue":"8","key":"pone.0249402.ref017","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1055\/s-0029-1145254","article-title":"Clinical utility of the PFA-100","volume":"34","author":"EJ Favaloro","year":"2008","journal-title":"Semin Thromb Hemost"},{"issue":"14","key":"pone.0249402.ref018","article-title":"Relationship between ABO blood group and von Willebrand factor levels: from biology to clinical implications","volume":"5","author":"M Franchini","year":"2007","journal-title":"Thromb J"},{"issue":"12","key":"pone.0249402.ref019","doi-asserted-by":"crossref","first-page":"5087","DOI":"10.1182\/blood-2006-12-027698","article-title":"The growing complexity of platelet aggregation","volume":"109","author":"SP Jackson","year":"2007","journal-title":"Blood"},{"issue":"5","key":"pone.0249402.ref020","first-page":"794","article-title":"A study of platelet functions with a new analyzer using high shear stress (PFA 100) in patients undergoing coronary artery bypass graft","volume":"84","author":"D Lasne","year":"2000","journal-title":"Thromb Haemost"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0249402","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,4,12]],"date-time":"2021-04-12T17:26:29Z","timestamp":1618248389000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0249402"}},"subtitle":[],"editor":[{"given":"Elizabeth S.","family":"Mayne","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2021,4,12]]},"references-count":20,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2021,4,12]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0249402","relation":{},"ISSN":["1932-6203"],"issn-type":[{"type":"electronic","value":"1932-6203"}],"subject":[],"published":{"date-parts":[[2021,4,12]]}}}