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The D-(+)-Galactose aging model is a consolidated methodology for studies of cardiovascular aging; however, its potential for use with ED remain unexplored. The present study proposed to characterize a new experimental model for ED, using the D-(+)-Galactose aging model. For the experiments, the animals were randomly divided into three groups receiving: vehicle (CTL), D-galactose 150 mg\/kg (DGAL), and D-(+)-galactose 150 mg\/Kg + sildenafil 1.5 mg\/Kg (DGAL+SD1.5) being administered daily for a period of eight weeks. All of the experimental protocols were previously approved by the Ethics Committee on the Use of Animals at the Federal University of Para\u00edba n\u00b0 9706070319. During the treatment, we analyzed physical, molecular, and physiological aspects related to the aging process and implicated in the development of ED. Our findings demonstrate for the first time that D-(+)-Galactose-induced aging represents a suitable experimental model for ED assessment. This was evidenced by an observed hyper-contractility in corpora cavernosa, significant endothelial dysfunction, increased ROS levels, an increase in cavernous tissue senescence, and the loss of essential penile erectile components.<\/jats:p>","DOI":"10.1371\/journal.pone.0249487","type":"journal-article","created":{"date-parts":[[2021,4,15]],"date-time":"2021-04-15T18:40:05Z","timestamp":1618512005000},"page":"e0249487","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":31,"title":["D-(+)-Galactose-induced aging: A novel experimental model of erectile dysfunction"],"prefix":"10.1371","volume":"16","author":[{"given":"Mathania Silva","family":"de Almeida Rezende","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2501-3249","authenticated-orcid":true,"given":"Arthur Jos\u00e9 Pontes","family":"Oliveira de 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