{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T11:41:27Z","timestamp":1793187687029,"version":"4.5.0"},"reference-count":36,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2021,9,21]],"date-time":"2021-09-21T00:00:00Z","timestamp":1632182400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"funder":[{"DOI":"10.13039\/100009168","name":"Animal and Plant Health Inspection Service","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009168","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006229","name":"Oak Ridge Institute for Science and Education","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006229","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>\n                    <jats:italic>Salmonella enterica<\/jats:italic>\n                    subspecies\n                    <jats:italic>enterica<\/jats:italic>\n                    serotype Dublin is a host-adapted serotype in cattle, associated with enteritis and systemic disease. The primary clinical manifestation of\n                    <jats:italic>Salmonella<\/jats:italic>\n                    Dublin infection in cattle, especially calves, is respiratory disease. While rare in humans, it can cause severe illness, including bacteremia, with hospitalization and death. In the United States,\n                    <jats:italic>S<\/jats:italic>\n                    . Dublin has become one of the most multidrug-resistant serotypes. The objective of this study was to characterize\n                    <jats:italic>S<\/jats:italic>\n                    . Dublin isolates from sick cattle by analyzing phenotypic and genotypic antimicrobial resistance (AMR) profiles, the presence of plasmids, and phylogenetic relationships.\n                    <jats:italic>S<\/jats:italic>\n                    . Dublin isolates (n = 140) were selected from submissions to the NVSL for\n                    <jats:italic>Salmonella<\/jats:italic>\n                    serotyping (2014\u20132017) from 21 states. Isolates were tested for susceptibility against 14 class-representative antimicrobial drugs. Resistance profiles were determined using the ABRicate with Resfinder and NCBI databases, AMRFinder and PointFinder. Plasmids were detected using ABRicate with PlasmidFinder. Phylogeny was determined using vSNP. We found 98% of the isolates were resistant to more than 4 antimicrobials. Only 1 isolate was pan-susceptible and had no predicted AMR genes. All\n                    <jats:italic>S<\/jats:italic>\n                    . Dublin isolates were susceptible to azithromycin and meropenem. They showed 96% resistance to sulfonamides, 97% to tetracyclines, 95% to aminoglycosides and 85% to beta-lactams. The most common AMR genes were: sulf2 and tetA (98.6%), aph(6)-Id (97.9%), aph(3\u2019\u2019)-Ib, (97.1%), floR (94.3%), and blaCMY-2 (85.7%). All quinolone resistant isolates presented mutations in\n                    <jats:italic>gyr<\/jats:italic>\n                    A. Ten plasmid types were identified among all isolates with IncA\/C2, IncX1, and IncFII(S) being the most frequent. The\n                    <jats:italic>S<\/jats:italic>\n                    . Dublin isolates show low genomic genetic diversity. This study provided antimicrobial susceptibility and genomic insight into\n                    <jats:italic>S<\/jats:italic>\n                    . Dublin clinical isolates from cattle in the U.S. Further sequence analysis integrating food and human origin\n                    <jats:italic>S<\/jats:italic>\n                    . Dublin isolates may provide valuable insight on increased virulence observed in humans.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0249617","type":"journal-article","created":{"date-parts":[[2021,9,21]],"date-time":"2021-09-21T14:23:40Z","timestamp":1632234220000},"page":"e0249617","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":41,"title":["Antimicrobial resistance and genomic characterization of Salmonella Dublin isolates in cattle from the United States"],"prefix":"10.1371","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7387-1793","authenticated-orcid":true,"given":"Mariela E.","family":"Srednik","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristina","family":"Lantz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica A.","family":"Hicks","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brenda R.","family":"Morningstar-Shaw","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tonya A.","family":"Mackie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda K.","family":"Schlater","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2021,9,21]]},"reference":[{"issue":"9","key":"pone.0249617.ref001","doi-asserted-by":"crossref","first-page":"1493","DOI":"10.3201\/eid2309.170136","article-title":"Epidemiology of Salmonella enterica Serotype Dublin Infections among Humans, United States, 1968\u20132013","volume":"23","author":"RR Harvey","year":"2017","journal-title":"Emerg Infect Dis"},{"issue":"1","key":"pone.0249617.ref002","doi-asserted-by":"crossref","first-page":"e01361","DOI":"10.1128\/MRA.01361-18","article-title":"Draft Genome Sequences of Salmonella enterica subsp. enterica Serovar Dublin Strains from St. Nectaire and Morbier Cheeses Characterized by Multilocus Variable-Number Tandem-Repeat Analysis Profiles Associated with Two Fatal Outbreaks in France","volume":"8","author":"S Cadel-Six","year":"2019","journal-title":"Microbiol Resour Announc"},{"issue":"13","key":"pone.0249617.ref003","article-title":"Evaluation of WGS-subtyping methods for epidemiological surveillance of foodborne salmonellosis","volume":"2","author":"M Mohammed","year":"2020","journal-title":"One Health Outlook"},{"key":"pone.0249617.ref004","unstructured":"CDC Centers for Disease Control and Prevention. 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