{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T05:37:04Z","timestamp":1773985024876,"version":"3.50.1"},"reference-count":63,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2021,4,14]],"date-time":"2021-04-14T00:00:00Z","timestamp":1618358400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000053","name":"National Eye Institute","doi-asserted-by":"publisher","award":["EY028222"],"award-info":[{"award-number":["EY028222"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Department of Medicine, University of Pittsburgh"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>\n                    <jats:italic>Enterococcus faecalis<\/jats:italic>\n                    are hospital-associated opportunistic pathogens and also causative agents of post-operative endophthalmitis. Patients with enterococcal endophthalmitis often have poor visual outcomes, despite appropriate antibiotic therapy. Here we investigated the genomic and phenotypic characteristics of\n                    <jats:italic>E<\/jats:italic>\n                    .\n                    <jats:italic>faecalis<\/jats:italic>\n                    isolates collected from 13 patients treated at the University of Pittsburgh Medical Center Eye Center over 19 years. Comparative genomic analysis indicated that patients were infected with\n                    <jats:italic>E<\/jats:italic>\n                    .\n                    <jats:italic>faecalis<\/jats:italic>\n                    belonging to diverse multi-locus sequence types (STs) and resembled\n                    <jats:italic>E<\/jats:italic>\n                    .\n                    <jats:italic>faecalis<\/jats:italic>\n                    sampled from clinical, commensal, and environmental sources. We identified known\n                    <jats:italic>E<\/jats:italic>\n                    .\n                    <jats:italic>faecalis<\/jats:italic>\n                    virulence factors and antibiotic resistance genes in each genome, including genes conferring resistance to aminoglycosides, erythromycin, and tetracyclines. We assessed all isolates for their cytolysin production, biofilm formation, and antibiotic susceptibility, and observed phenotypic differences between isolates. Fluoroquinolone and cephalosporin susceptibilities were particularly variable between isolates, as were biofilm formation and cytolysin production. In addition, we found evidence of\n                    <jats:italic>E<\/jats:italic>\n                    .\n                    <jats:italic>faecalis<\/jats:italic>\n                    adaptation during recurrent endophthalmitis by identifying genetic variants that arose in sequential isolates sampled over eight months from the same patient. We identified a mutation in the DNA mismatch repair gene\n                    <jats:italic>mutS<\/jats:italic>\n                    that was associated with an increased rate of spontaneous mutation in the final isolate from the patient. Overall this study documents the genomic and phenotypic variability among\n                    <jats:italic>E<\/jats:italic>\n                    .\n                    <jats:italic>faecalis<\/jats:italic>\n                    causing endophthalmitis, as well as possible adaptive mechanisms underlying bacterial persistence during recurrent ocular infection.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0250084","type":"journal-article","created":{"date-parts":[[2021,4,14]],"date-time":"2021-04-14T18:28:28Z","timestamp":1618424908000},"page":"e0250084","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":16,"title":["Genomic and phenotypic diversity of Enterococcus faecalis isolated from endophthalmitis"],"prefix":"10.1371","volume":"16","author":[{"given":"Gayatri Shankar","family":"Chilambi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hayley R.","family":"Nordstrom","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel R.","family":"Evans","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Regis P.","family":"Kowalski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deepinder K.","family":"Dhaliwal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vishal","family":"Jhanji","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert M. Q.","family":"Shanks","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7284-0103","authenticated-orcid":true,"given":"Daria","family":"Van Tyne","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2021,4,14]]},"reference":[{"key":"pone.0250084.ref001","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1146\/annurev-micro-091213-113003","article-title":"Friend Turned Foe: Evolution of Enterococcal Virulence and Antibiotic Resistance","volume":"68","author":"D Van Tyne","year":"2014","journal-title":"Annu Rev Microbiol"},{"key":"pone.0250084.ref002","doi-asserted-by":"crossref","first-page":"e00058","DOI":"10.1128\/CMR.00058-18","article-title":"The Enterococcus: a Model of Adaptability to Its Environment","volume":"32","author":"M Garc\u00eda-Solache","year":"2019","journal-title":"Clin Microbiol Rev"},{"key":"pone.0250084.ref003","doi-asserted-by":"crossref","DOI":"10.1128\/microbiolspec.GPP3-0053-2018","article-title":"Pathogenicity of Enterococci.","volume":"7","author":"E Fiore","year":"2019","journal-title":"Microbiol Spectr"},{"key":"pone.0250084.ref004","first-page":"1","volume-title":"Enterococcal Infection\u2014Treatment and Antibiotic Resistance. Enterococci: From Commensals to Leading Causes of Drug Resistant Infection.","author":"CJ Kristich","year":"2014"},{"key":"pone.0250084.ref005","doi-asserted-by":"crossref","first-page":"e00064","DOI":"10.1128\/mSphere.00064-16","article-title":"CRISPR-Cas and Restriction-Modification Act Additively against Conjugative Antibiotic Resistance Plasmid Transfer in Enterococcus faecalis","volume":"1","author":"VJ Price","year":"2016","journal-title":"mSphere"},{"key":"pone.0250084.ref006","doi-asserted-by":"crossref","first-page":"577","DOI":"10.2307\/30141097","article-title":"Recovery of vancomycin-resistant enterococci on fingertips and environmental surfaces.","volume":"16","author":"GA Noskin","year":"1995","journal-title":"Infect Control Hosp Epidemiol"},{"key":"pone.0250084.ref007","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1128\/CMR.15.1.111-124.2002","article-title":"Bacterial endophthalmitis: epidemiology, therapeutics, and bacterium-host interactions","volume":"15","author":"MC Callegan","year":"2002","journal-title":"Clin Microbiol Rev"},{"key":"pone.0250084.ref008","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1038\/eye.2010.236","article-title":"Microbial profile and antibiotic susceptibility of culture-positive bacterial endophthalmitis.","volume":"25","author":"GB Melo","year":"2011","journal-title":"Eye"},{"key":"pone.0250084.ref009","doi-asserted-by":"crossref","first-page":"1018","DOI":"10.1016\/j.ajo.2014.07.038","article-title":"Endophthalmitis Caused by Enterococcus faecalis: Clinical Features, Antibiotic Sensitivities, and Outcomes","volume":"158","author":"AE Kuriyan","year":"2014","journal-title":"Am J Ophthalmol"},{"key":"pone.0250084.ref010","first-page":"39","article-title":"Six-year incidence of endophthalmitis after cataract surgery: Swedish national study.","author":"E Friling","year":"2013","journal-title":"J Cataract Refract Surg"},{"key":"pone.0250084.ref011","doi-asserted-by":"crossref","first-page":"1892","DOI":"10.1080\/22221751.2020.1810134","article-title":"Emerging Enterococcus isolates in postoperative endophthalmitis by selection pressure of fluoroquinolones: an 11-year multicenter and experimental study","volume":"9","author":"JK Kim","year":"2020","journal-title":"Emerg Microbes Infect."},{"key":"pone.0250084.ref012","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1007\/s10792-016-0288-2","article-title":"Isolates and antibiotic susceptibilities of endophthalmitis in postcataract surgery: a 12-year review of culture-proven cases.","volume":"37","author":"YT Teng","year":"2017","journal-title":"Int Ophthalmol."},{"key":"pone.0250084.ref013","doi-asserted-by":"crossref","first-page":"1355","DOI":"10.1016\/j.jcrs.2011.04.014","article-title":"Recurrent enterococcal endophthalmitis seeded by an intraocular lens biofilm.","volume":"37","author":"V Miller K","year":"2011","journal-title":"J Cataract Refract Surg"},{"key":"pone.0250084.ref014","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1089\/cmb.2012.0021","article-title":"SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing","volume":"19","author":"A Bankevich","year":"2012","journal-title":"J Comput Biol"},{"key":"pone.0250084.ref015","doi-asserted-by":"crossref","first-page":"2068","DOI":"10.1093\/bioinformatics\/btu153","article-title":"Prokka: rapid prokaryotic genome annotation","volume":"30","author":"T. Seemann","year":"2014","journal-title":"Bioinformatics"},{"key":"pone.0250084.ref016","doi-asserted-by":"crossref","first-page":"3691","DOI":"10.1093\/bioinformatics\/btv421","article-title":"Roary: rapid large-scale prokaryote pan genome analysis","volume":"31","author":"AJ Page","year":"2015","journal-title":"Bioinformatics"},{"key":"pone.0250084.ref017","doi-asserted-by":"crossref","first-page":"1312","DOI":"10.1093\/bioinformatics\/btu033","article-title":"RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies","volume":"30","author":"A. Stamatakis","year":"2014","journal-title":"Bioinformatics"},{"key":"pone.0250084.ref018","doi-asserted-by":"crossref","first-page":"W246","DOI":"10.1093\/nar\/gky425","article-title":"CRISPRCasFinder, an update of CRISRFinder, includes a portable version, enhanced performance and integrates search for Cas proteins","volume":"46","author":"D Couvin","year":"2018","journal-title":"Nucleic Acids Res"},{"key":"pone.0250084.ref019","doi-asserted-by":"crossref","first-page":"W16","DOI":"10.1093\/nar\/gkw387","article-title":"PHASTER: a better, faster version of the PHAST phage search tool","volume":"44","author":"D Arndt","year":"2016","journal-title":"Nucleic Acids Res"},{"key":"pone.0250084.ref020","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1038\/nprot.2007.521","article-title":"Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances.","volume":"3","author":"I Wiegand","year":"2008","journal-title":"Nat Protoc."},{"key":"pone.0250084.ref021","unstructured":"CLSI. Performance Standards for Antimicrobial Susceptibility Testing. 29th ed. CLSI supplement M100. Clinical and Laboratory Standards Institute. 2019."},{"key":"pone.0250084.ref022","article-title":"Microtiter Dish Biofilm Formation Assay.","volume":"2437","author":"GA O\u2019Toole","year":"2011","journal-title":"J Vis Exp."},{"key":"pone.0250084.ref023","doi-asserted-by":"crossref","first-page":"e01336","DOI":"10.1128\/AEM.01336-18","article-title":"Long-Term Colonization Dynamics of Enterococcus faecalis in Implanted Devices in Research Macaques.","volume":"84","author":"MT Lieberman","year":"2018","journal-title":"Appl Environ Microbiol"},{"key":"pone.0250084.ref024","doi-asserted-by":"crossref","first-page":"4126","DOI":"10.1038\/s41598-018-22416-4","article-title":"Genomics-Based Identification of Microorganisms in Human Ocular Body Fluid.","volume":"8","author":"P Kirstahler","year":"2018","journal-title":"Sci Rep."},{"key":"pone.0250084.ref025","doi-asserted-by":"crossref","first-page":"e582","DOI":"10.1371\/journal.pone.0000582","article-title":"Genetic diversity among Enterococcus faecalis.","volume":"2","author":"SM McBride","year":"2007","journal-title":"PLoS One"},{"key":"pone.0250084.ref026","doi-asserted-by":"crossref","first-page":"2640","DOI":"10.1093\/jac\/dks261","article-title":"Identification of acquired antimicrobial resistance genes","volume":"67","author":"E Zankari","year":"2012","journal-title":"J Antimicrob Chemother"},{"key":"pone.0250084.ref027","doi-asserted-by":"crossref","first-page":"3895","DOI":"10.1128\/AAC.02412-14","article-title":"In silico detection and typing of plasmids using PlasmidFinder and plasmid multilocus sequence typing","volume":"58","author":"A Carattoli","year":"2014","journal-title":"Antimicrob Agents Chemother"},{"key":"pone.0250084.ref028","doi-asserted-by":"crossref","first-page":"W52","DOI":"10.1093\/nar\/gkm360","article-title":"CRISPRFinder: a web tool to identify clustered regularly interspaced short palindromic repeats","volume":"35","author":"I Grissa","year":"2007","journal-title":"Nucleic Acids Res"},{"key":"pone.0250084.ref029","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1007\/s00248-013-0217-0","article-title":"CRISPRs of Enterococcus faecalis and E. hirae Isolates from Pig Feces Have Species-Specific Repeats But Share Some Common Spacer Sequences.","volume":"66","author":"I Katyal","year":"2013","journal-title":"Microb Ecol."},{"key":"pone.0250084.ref030","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1186\/s12866-017-0962-1","article-title":"Comparative genomics of Enterococcus spp. isolated from bovine feces","volume":"17","author":"AG Beukers","year":"2017","journal-title":"BMC Microbiol"},{"key":"pone.0250084.ref031","doi-asserted-by":"crossref","first-page":"1628","DOI":"10.1128\/AEM.67.4.1628-1635.2001","article-title":"Molecular screening of Enterococcus virulence determinants and potential for genetic exchange between food and medical isolates","volume":"67","author":"TJ Eaton","year":"2001","journal-title":"Appl Environ Microbiol"},{"key":"pone.0250084.ref032","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1016\/S0168-1605(02)00425-7","article-title":"Screening for enterocins and detection of hemolysin and vancomycin resistance in enterococci of different origins","volume":"84","author":"L De Vuyst","year":"2003","journal-title":"Int J Food Microbiol"},{"key":"pone.0250084.ref033","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1016\/j.ijfoodmicro.2010.10.024","article-title":"The occurrence of virulence traits among high-level aminoglycosides resistant Enterococcus isolates obtained from feces of humans, animals, and birds in South Korea","volume":"144","author":"D Han","year":"2011","journal-title":"Int J Food Microbiol"},{"key":"pone.0250084.ref034","doi-asserted-by":"crossref","first-page":"895","DOI":"10.3390\/toxins5050895","article-title":"Structure, function, and biology of the Enterococcus faecalis cytolysin.","volume":"5","author":"D Van Tyne","year":"2013","journal-title":"Toxins (Basel)."},{"key":"pone.0250084.ref035","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1046\/j.1462-5822.2003.00310.x","article-title":"The Enterococcus faecalis cytolysin: a novel toxin active against eukaryotic and prokaryotic cells","volume":"5","author":"PS Coburn","year":"2003","journal-title":"Cell Microbiol"},{"key":"pone.0250084.ref036","doi-asserted-by":"crossref","first-page":"1501","DOI":"10.1128\/JCM.03617-13","article-title":"Real-time whole-genome sequencing for routine typing, surveillance, and outbreak detection of verotoxigenic Escherichia coli","volume":"52","author":"KG Joensen","year":"2014","journal-title":"J Clin Microbiol"},{"key":"pone.0250084.ref037","doi-asserted-by":"crossref","DOI":"10.1099\/mgen.0.000350","article-title":"Comparative genomics of global optrA-carrying Enterococcus faecalis uncovers a common chromosomal hotspot for optrA acquisition within a diversity of core and accessory genomes","volume":"6","author":"AR Freitas","year":"2020","journal-title":"Microb genomics"},{"key":"pone.0250084.ref038","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1038\/s41579-018-0107-z","article-title":"Biofilm-associated infection by enterococci","volume":"17","author":"J-H Ch\u2019ng","year":"2019","journal-title":"Nat Rev Microbiol"},{"key":"pone.0250084.ref039","doi-asserted-by":"crossref","first-page":"15985","DOI":"10.1074\/jbc.M116.726562","article-title":"Structural and Functional Analysis of Cell Wall-anchored Polypeptide Adhesin BspA in Streptococcus agalactiae","volume":"291","author":"S Rego","year":"2016","journal-title":"J Biol Chem"},{"key":"pone.0250084.ref040","doi-asserted-by":"crossref","first-page":"620","DOI":"10.1046\/j.1365-2958.1999.01631.x","article-title":"Cloning and functional analysis of Asa373, a novel adhesin unrelated to the other sex pheromone plasmid-encoded aggregation substances of Enterococcus faecalis.","volume":"34","author":"A Muscholl-Silberhorn","year":"1999","journal-title":"Mol Microbiol"},{"key":"pone.0250084.ref041","doi-asserted-by":"crossref","first-page":"1800","DOI":"10.1128\/AAC.46.6.1800-1804.2002","article-title":"Inhibitory Activities of Quinolones against DNA Gyrase and Topoisomerase IV of Enterococcus faecalis","volume":"46","author":"Y Onodera","year":"2002","journal-title":"Antimicrob Agents Chemother"},{"key":"pone.0250084.ref042","doi-asserted-by":"crossref","first-page":"3061","DOI":"10.1128\/AAC.47.10.3061-3066.2003","article-title":"Mutations in the DNA Mismatch Repair Proteins MutS and MutL of Oxazolidinone-Resistant or -Susceptible Enterococcus faecium","volume":"47","author":"RJ Willems","year":"2003","journal-title":"Antimicrob Agents Chemother"},{"key":"pone.0250084.ref043","doi-asserted-by":"crossref","first-page":"699","DOI":"10.1007\/s10384-018-0630-5","article-title":"Genetic diversity and persistent colonization of Enterococcus faecalis on ocular surfaces","volume":"62","author":"D Todokoro","year":"2018","journal-title":"Jpn J Ophthalmol"},{"key":"pone.0250084.ref044","doi-asserted-by":"crossref","first-page":"e0208518","DOI":"10.1371\/journal.pone.0208518","article-title":"Staphylococcus aureus from ocular and otolaryngology infections are frequently resistant to clinically important antibiotics and are associated with lineages of community and hospital origins.","volume":"13","author":"JI Wurster","year":"2018","journal-title":"PLoS One"},{"key":"pone.0250084.ref045","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/j.tim.2009.03.002","article-title":"A modular master on the move: the Tn916 family of mobile genetic elements","volume":"17","author":"AP Roberts","year":"2009","journal-title":"Trends Microbiol"},{"key":"pone.0250084.ref046","doi-asserted-by":"crossref","first-page":"586","DOI":"10.1086\/313949","article-title":"Aminoglycoside Resistance in Enterococci","volume":"31","author":"JW Chow","year":"2000","journal-title":"Clin Infect Dis"},{"key":"pone.0250084.ref047","doi-asserted-by":"crossref","first-page":"e0121189","DOI":"10.1371\/journal.pone.0121189","article-title":"Unraveling antimicrobial resistance genes and phenotype patterns among Enterococcus faecalis isolated from retail chicken products in Japan.","volume":"10","author":"A Hidano","year":"2015","journal-title":"PLoS One"},{"key":"pone.0250084.ref048","doi-asserted-by":"crossref","first-page":"421","DOI":"10.4161\/viru.21282","article-title":"Intrinsic and acquired resistance mechanisms in enterococcus.","volume":"3","author":"BL Hollenbeck","year":"2012","journal-title":"Virulence"},{"key":"pone.0250084.ref049","doi-asserted-by":"crossref","first-page":"e00227","DOI":"10.1128\/mBio.00227-10","article-title":"Multidrug-Resistant Enterococci Lack CRISPR.","volume":"1","author":"KL Palmer","year":"2010","journal-title":"MBio"},{"key":"pone.0250084.ref050","doi-asserted-by":"crossref","first-page":"e00464","DOI":"10.1128\/mSphere.00464-19","article-title":"Enterococcus faecalis CRISPR-Cas Is a Robust Barrier to Conjugative Antibiotic Resistance Dissemination in the Murine Intestine.","volume":"4","author":"VJ Price","year":"2019","journal-title":"mSphere"},{"key":"pone.0250084.ref051","doi-asserted-by":"crossref","first-page":"2445","DOI":"10.1128\/IAI.60.6.2445-2452.1992","article-title":"Contribution of the pAD1-encoded cytolysin to the severity of experimental Enterococcus faecalis endophthalmitis","volume":"60","author":"BD Jett","year":"1992","journal-title":"Infect Immun"},{"key":"pone.0250084.ref052","doi-asserted-by":"crossref","first-page":"4678","DOI":"10.1128\/IAI.70.8.4678-4681.2002","article-title":"The Enterococcus faecalis fsrB gene, a key component of the fsr quorum-sensing system, is associated with virulence in the rabbit endophthalmitis model","volume":"70","author":"E Mylonakis","year":"2002","journal-title":"Infect Immun"},{"key":"pone.0250084.ref053","doi-asserted-by":"crossref","first-page":"3628","DOI":"10.1128\/IAI.72.6.3628-3633.2004","article-title":"Contribution of gelatinase, serine protease, and fsr to the pathogenesis of Enterococcus faecalis endophthalmitis","volume":"72","author":"M Engelbert","year":"2004","journal-title":"Infect Immun"},{"key":"pone.0250084.ref054","doi-asserted-by":"crossref","first-page":"e23500","DOI":"10.1371\/journal.pone.0023500","article-title":"Isolation of Genes Involved in Biofilm Formation of a Klebsiella pneumoniae Strain Causing Pyogenic Liver Abscess.","volume":"6","author":"M-C Wu","year":"2011","journal-title":"PLoS One."},{"key":"pone.0250084.ref055","doi-asserted-by":"crossref","first-page":"622","DOI":"10.1016\/j.jcrs.2004.06.047","article-title":"Recurrent Enterococcus faecalis endophthalmitis after phacoemulsification.","volume":"31","author":"SCB Teoh","year":"2005","journal-title":"J Cataract Refract Surg"},{"key":"pone.0250084.ref056","doi-asserted-by":"crossref","first-page":"5218","DOI":"10.1128\/IAI.68.9.5218-5224.2000","article-title":"Enterococcus faecalis adhesin, ace, mediates attachment to extracellular matrix proteins collagen type IV and laminin as well as collagen type I","volume":"68","author":"SR Nallapareddy","year":"2000","journal-title":"Infect Immun"},{"key":"pone.0250084.ref057","doi-asserted-by":"crossref","first-page":"e1000716","DOI":"10.1371\/journal.ppat.1000716","article-title":"Importance of the collagen adhesin ace in pathogenesis and protection against Enterococcus faecalis experimental endocarditis.","volume":"6","author":"V Singh K","year":"2010","journal-title":"PLoS Pathog."},{"key":"pone.0250084.ref058","doi-asserted-by":"crossref","first-page":"2832","DOI":"10.1128\/IAI.01218-08","article-title":"ace, Which Encodes an Adhesin in Enterococcus faecalis, Is Regulated by Ers and Is Involved in Virulence","volume":"77","author":"F Lebreton","year":"2009","journal-title":"Infect Immun"},{"key":"pone.0250084.ref059","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/S0161-6420(01)00899-5","article-title":"Bacterial endophthalmitis prophylaxis for cataract surgery: An evidence-based update","volume":"109","author":"TA Ciulla","year":"2002","journal-title":"Ophthalmology"},{"key":"pone.0250084.ref060","doi-asserted-by":"crossref","first-page":"e01088","DOI":"10.1128\/AAC.01088-19","article-title":"Therapeutic Effects of Intravitreously Administered Bacteriophage in a Mouse Model of Endophthalmitis Caused by Vancomycin-Sensitive or -Resistant Enterococcus faecalis","volume":"63","author":"T Kishimoto","year":"2019","journal-title":"Antimicrob Agents Chemother"},{"key":"pone.0250084.ref061","doi-asserted-by":"crossref","first-page":"7184","DOI":"10.1128\/JB.185.24.7184-7192.2003","article-title":"The CroRS Two-Component Regulatory System Is Requiredfor Intrinsic \u03b2-Lactam Resistance in Enterococcusfaecalis","volume":"185","author":"Y Comenge","year":"2003","journal-title":"J Bacteriol"},{"key":"pone.0250084.ref062","doi-asserted-by":"crossref","first-page":"3508","DOI":"10.1073\/pnas.0608742104","article-title":"A eukaryotic-type Ser\/Thr kinase in Enterococcus faecalis mediates antimicrobial resistance and intestinal persistence","volume":"104","author":"CJ Kristich","year":"2007","journal-title":"Proc Natl Acad Sci"},{"key":"pone.0250084.ref063","doi-asserted-by":"crossref","first-page":"e00199","DOI":"10.1128\/mBio.00199-11","article-title":"Reciprocal Regulation of Cephalosporin Resistance in Enterococcus faecalis.","volume":"2","author":"CJ Kristich","year":"2011","journal-title":"MBio"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0250084","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,4,14]],"date-time":"2021-04-14T18:29:23Z","timestamp":1618424963000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0250084"}},"subtitle":[],"editor":[{"given":"Iddya","family":"Karunasagar","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2021,4,14]]},"references-count":63,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2021,4,14]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0250084","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2020.08.05.239061","asserted-by":"object"}]},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,4,14]]}}}