{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T19:09:49Z","timestamp":1774552189328,"version":"3.50.1"},"reference-count":38,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2021,4,22]],"date-time":"2021-04-22T00:00:00Z","timestamp":1619049600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004329","name":"Slovenian Research Agency","doi-asserted-by":"crossref","award":["P3-0296"],"award-info":[{"award-number":["P3-0296"]}],"id":[{"id":"10.13039\/501100004329","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Neither pre-treatment characteristics, nor the outcome after antibiotic therapy, have been reported for spirochetemic European patients with Lyme borreliosis. In the present study, patients with a solitary erythema migrans (EM) who had a positive blood culture for either<jats:italic>Borrelia afzelii<\/jats:italic>(n = 116) or<jats:italic>Borrelia garinii<\/jats:italic>(n = 37) were compared with age- and sex-matched patients who had a negative blood culture, but were culture positive for the corresponding<jats:italic>Borrelia<\/jats:italic>species from skin. Collectively, spirochetemic patients significantly more often recalled a tick bite at the site of the EM skin lesion, had a shorter time interval from the bite to the onset of EM, had a shorter duration of the skin lesion prior to diagnosis, and had a smaller EM skin lesion that was more often homogeneous in appearance. Similar results were found for the subset of spirochetemic patients infected with<jats:italic>B<\/jats:italic>.<jats:italic>afzelii<\/jats:italic>but not for those infected with<jats:italic>B<\/jats:italic>.<jats:italic>garinii<\/jats:italic>. However, patients with<jats:italic>B<\/jats:italic>.<jats:italic>garinii<\/jats:italic>bacteremia had faster-spreading and larger EM skin lesions, and more often reported itching at the site of the lesion than patients with<jats:italic>B<\/jats:italic>.<jats:italic>afzelii<\/jats:italic>bacteremia. Treatment failures were rare (7\/306 patients, 2.3%) and were not associated with having spirochetemia or with which<jats:italic>Borrelia<\/jats:italic>species was causing the infection.<\/jats:p>","DOI":"10.1371\/journal.pone.0250198","type":"journal-article","created":{"date-parts":[[2021,4,22]],"date-time":"2021-04-22T20:17:03Z","timestamp":1619122623000},"page":"e0250198","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":8,"title":["Characteristics of spirochetemic patients with a solitary erythema migrans skin lesion in Europe"],"prefix":"10.1371","volume":"16","author":[{"given":"Vera","family":"Maraspin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katarina","family":"Ogrinc","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tereza","family":"Rojko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Petra","family":"Bogovi\u010d","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eva","family":"Ru\u017ei\u0107-Sablji\u0107","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3495-0165","authenticated-orcid":true,"given":"Andrej","family":"Kastrin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gary P.","family":"Wormser","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4163-0503","authenticated-orcid":true,"given":"Franc","family":"Strle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2021,4,22]]},"reference":[{"key":"pone.0250198.ref001","doi-asserted-by":"crossref","first-page":"16090","DOI":"10.1038\/nrdp.2016.90","article-title":"Lyme borreliosis","volume":"2","author":"AC Steere","year":"2016","journal-title":"Nat Rev Dis Primers"},{"issue":"9814","key":"pone.0250198.ref002","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1016\/S0140-6736(11)60103-7","article-title":"Lyme borreliosis","volume":"379","author":"G Stanek","year":"2012","journal-title":"Lancet"},{"issue":"3","key":"pone.0250198.ref003","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1093\/femsre\/fux047","article-title":"Lyme borreliosis-from tick bite to diagnosis and treatment","volume":"42","author":"G Stanek","year":"2018","journal-title":"FEMS Microbiol Rev"},{"issue":"2","key":"pone.0250198.ref004","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1007\/s15010-001-0154-6","article-title":"Isolation of Borrelia burgdorferi sensu lato from blood of patients with erythema migrans","volume":"29","author":"V Maraspin","year":"2001","journal-title":"Infection. 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