{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T21:17:28Z","timestamp":1791839848095,"version":"4.3.4"},"reference-count":54,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2021,4,20]],"date-time":"2021-04-20T00:00:00Z","timestamp":1618876800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008804","name":"Ministry of Science and Technology, Government of the People\u2019s Republic of Bangladesh","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008804","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Obesity is an enduring medical issue that has raised concerns around the world. Natural plant extracts have shown therapeutic potential in preventing oxidative stress and inflammation related to obesity complications. In this study,<jats:italic>Senna alexandrina<\/jats:italic>Mill. leaves were utilized to treat high-fat diet-related metabolic disorders and non-alcoholic fatty liver diseases. Plasma biochemical assays were conducted to determine the lipid profiles and oxidative stress parameters, and the gene expression of antioxidant enzymes and inflammatory mediators was measured. Histological stained livers of high-fat diet-fed rats were observed.<jats:italic>S<\/jats:italic>.<jats:italic>alexandrina<\/jats:italic>leaf powder supplementation prevented the increase in cholesterol and triglyceride levels in high-fat diet-fed rats. Moreover,<jats:italic>S<\/jats:italic>.<jats:italic>alexandrina<\/jats:italic>leaves also reduced lipid peroxidation and nitric oxide production in these rats. Prevention of oxidative stress by<jats:italic>S<\/jats:italic>.<jats:italic>alexandrina<\/jats:italic>leaf supplementation in high-fat diet-fed rats is regulated by enhancing the antioxidant enzyme activity, followed by the restoration of corresponding gene expressions, such as<jats:italic>NRF-2<\/jats:italic>,<jats:italic>HO-1<\/jats:italic>,<jats:italic>SOD<\/jats:italic>, and<jats:italic>CAT<\/jats:italic>. Histological staining provides further evidence that<jats:italic>S<\/jats:italic>.<jats:italic>alexandrina<\/jats:italic>leaf supplementation prevents inflammatory cell infiltration, lipid droplet deposition, and fibrosis in the liver of high-fat diet-fed rats. Furthermore, this investigation revealed that<jats:italic>S<\/jats:italic>.<jats:italic>alexandrina<\/jats:italic>leaf supplementation controlled non-alcoholic fatty liver disease by modulating the expression of fat metabolizing enzymes in high-fat diet-fed rats. Therefore,<jats:italic>S<\/jats:italic>.<jats:italic>alexandrina<\/jats:italic>leaf supplementation inhibits fatty liver inflammation and fibrosis, suggesting its usefulness in treating non-alcoholic steatohepatitis. Thus, this natural leaf extract has potential in treatment of obesity related liver dysfunction.<\/jats:p>","DOI":"10.1371\/journal.pone.0250261","type":"journal-article","created":{"date-parts":[[2021,4,20]],"date-time":"2021-04-20T19:06:02Z","timestamp":1618945562000},"page":"e0250261","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":16,"title":["Leaf powder supplementation of Senna alexandrina ameliorates oxidative stress, inflammation, and hepatic steatosis in high-fat diet-fed obese rats"],"prefix":"10.1371","volume":"16","author":[{"given":"Shariful Islam","family":"Nayan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Faizul Islam","family":"Chowdhury","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noushin","family":"Akter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md Mizanur","family":"Rahman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saima","family":"Selim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadia","family":"Saffoon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ferdous","family":"Khan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nusrat","family":"Subhan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maqsud","family":"Hossain","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. 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