{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T12:59:48Z","timestamp":1778504388845,"version":"3.51.4"},"reference-count":63,"publisher":"Public Library of Science (PLoS)","issue":"4","license":[{"start":{"date-parts":[[2021,4,20]],"date-time":"2021-04-20T00:00:00Z","timestamp":1618876800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["15K08144"],"award-info":[{"award-number":["15K08144"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["17H00860"],"award-info":[{"award-number":["17H00860"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["17dm0107066h"],"award-info":[{"award-number":["17dm0107066h"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"name":"The Center of Innovation (COI) Program of Japanese Science and Technology Agency","award":["JPMJCE1305"],"award-info":[{"award-number":["JPMJCE1305"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Valproic acid (VPA) treatment is associated with autism spectrum disorder in humans, and ferrets can be used as a model to test this; so far, it is not known whether ferrets react to developmental VPA exposure with gyrencephalic abnormalities. The current study characterized gyrification abnormalities in ferrets following VPA exposure during neonatal periods, corresponding to the late stage of cortical neurogenesis as well as the early stage of sulcogyrogenesis. Ferret pups received intraperitoneal VPA injections (200 \u03bcg\/g of body weight) on postnatal days (PD) 6 and 7. BrdU was administered simultaneously at the last VPA injection. <jats:italic>Ex vivo<\/jats:italic> MRI-based morphometry demonstrated significantly lower gyrification index (GI) throughout the cortex in VPA-treated ferrets (1.265 \u00b1 0.027) than in control ferrets (1.327 \u00b1 0.018) on PD 20, when primary sulcogyrogenesis is complete. VPA-treated ferrets showed significantly smaller sulcal-GIs in the rostral suprasylvian sulcus and splenial sulcus but a larger lateral sulcus surface area than control ferrets. The floor cortex of the inner stratum of both the rostral suprasylvian and splenial sulci and the outer stratum of the lateral sulcus showed a relatively prominent expansion. Parvalbumin-positive neuron density was significantly greater in the expanded cortical strata of sulcal floors in VPA-treated ferrets, regardless of the BrdU-labeled status. Thus, VPA exposure during the late stage of cortical neurogenesis may alter gyrification, primarily in the frontal and parietotemporal cortical divisions. Altered gyrification may thicken the outer or inner stratum of the cerebral cortex by increasing parvalbumin-positive neuron density.<\/jats:p>","DOI":"10.1371\/journal.pone.0250262","type":"journal-article","created":{"date-parts":[[2021,4,20]],"date-time":"2021-04-20T20:38:44Z","timestamp":1618951124000},"page":"e0250262","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":18,"title":["Neonatal valproic acid exposure produces altered gyrification related to increased parvalbumin-immunopositive neuron density with thickened sulcal floors"],"prefix":"10.1371","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8402-1041","authenticated-orcid":true,"given":"Kazuhiko","family":"Sawada","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shiori","family":"Kamiya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ichio","family":"Aoki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2021,4,20]]},"reference":[{"key":"pone.0250262.ref001","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1002\/ana.410010109","article-title":"Gyral development of the human brain","volume":"1","author":"JG Chi","year":"1977","journal-title":"Annals of Neurology"},{"key":"pone.0250262.ref002","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1016\/j.neuroimage.2007.11.021","article-title":"Study of the development of fetal baboon brain using magnetic resonance imaging at 3 Tesla","volume":"40","author":"F Liu","year":"2008","journal-title":"Neuroimage"},{"key":"pone.0250262.ref003","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1007\/s00429-006-0136-7","article-title":"Development of cerebral sulci and gyri in fetuses of cynomolgus monkeys (Macaca fascicularis)","volume":"211","author":"K Fukunishi","year":"2006","journal-title":"Anatomy and Embryology (Berlin)"},{"key":"pone.0250262.ref004","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1007\/s00429-009-0204-x","article-title":"Developments of sulcal pattern and subcortical structures of the forebrain in cynomolgus monkey fetuses: 7-tesla magnetic resonance imaging provides high reproducibility of gross structural changes","volume":"213","author":"K Sawada","year":"2009","journal-title":"Brain Structure and Function"},{"key":"pone.0250262.ref005","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1111\/j.1741-4520.2011.00352.x","article-title":"Neuroanatomic and MRI references for normal development of cerebral sulci of laboratory primate, cynomolgus monkeys (Macaca fascicularis)","volume":"52","author":"K Sawada","year":"2012","journal-title":"Congenital Anomalies (Kyoto)"},{"key":"pone.0250262.ref006","doi-asserted-by":"crossref","first-page":"1065","DOI":"10.1002\/ar.22478","article-title":"Fetal gyrification in cynomolgus monkeys: a concept of developmental stages of gyrification","volume":"295","author":"K Sawada","year":"2012","journal-title":"Anatomical Record (Hoboken)"},{"key":"pone.0250262.ref007","doi-asserted-by":"crossref","first-page":"1509","DOI":"10.3390\/sym12091509","article-title":"Cerebral sulcal asymmetry in macaque monkeys","volume":"12","author":"K Sawada","year":"2021","journal-title":"Symmetry"},{"key":"pone.0250262.ref008","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1016\/j.neuroscience.2013.10.067","article-title":"Fetal sulcation and gyrification in common marmosets (Callithrix jacchus) obtained by ex vivo magnetic resonance imaging","volume":"257","author":"K Sawada","year":"2014","journal-title":"Neuroscience"},{"key":"pone.0250262.ref009","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1111\/j.1439-0264.1989.tb00600.x","article-title":"The development of the sulci and gyri of the bovine cerebral hemispheres","volume":"18","author":"GJ Louw","year":"1989","journal-title":"Anatomia, Histolologia, Embryologia"},{"key":"pone.0250262.ref010","first-page":"441","article-title":"Quantitative analysis of gyrification of cerebral cortex in dogs","volume":"4","author":"M Wosinski","year":"1996","journal-title":"Neurobiology"},{"key":"pone.0250262.ref011","first-page":"141","article-title":"Gyrus formation in the cerebral cortex in the ferret. I. Description of the external histological changes","volume":"146","author":"IH Smart","year":"1986","journal-title":"Journal of Anatomy"},{"key":"pone.0250262.ref012","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1111\/j.1741-4520.2012.00372.x","article-title":"Development of cerebral sulci and gyri in ferrets (Mustela putorius)","volume":"52","author":"K Sawada","year":"2012","journal-title":"Congenital Anomalies (Kyoto)"},{"key":"pone.0250262.ref013","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.neuroscience.2017.09.015","article-title":"Biphasic aspect of sexually dimorphic ontogenetic trajectory of gyrification in the ferret cerebral cortex","volume":"364","author":"K Sawada","year":"2017","journal-title":"Neuroscience"},{"key":"pone.0250262.ref014","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1093\/cercor\/bhr301","article-title":"Abundant occurrence of basal radial glia in the subventricular zone of embryonic neocortex of a lissencephalic primate, the common marmoset Callithrix jacchus","volume":"22","author":"I Kelava","year":"2012","journal-title":"Cerebral Cortex"},{"key":"pone.0250262.ref015","doi-asserted-by":"crossref","first-page":"1462","DOI":"10.1177\/0883073810368311","article-title":"Cortical gyrification in autistic and Asperger disorders: a preliminary magnetic resonance imaging study","volume":"25","author":"RJ Jou","year":"2010","journal-title":"Journal of Child Neurology"},{"key":"pone.0250262.ref016","doi-asserted-by":"crossref","first-page":"1956","DOI":"10.1093\/brain\/awt106","article-title":"Increased gyrification, but comparable surface area in adolescents with autism spectrum disorders","volume":"136","author":"GL Wallace","year":"2013","journal-title":"Brain"},{"key":"pone.0250262.ref017","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.neuropsychologia.2014.07.001","article-title":"Surface-based morphometry of the cortical architecture of autism spectrum disorders: volume, thickness, area, and gyrification","volume":"62","author":"LE Libero","year":"2014","journal-title":"Neuropsychologia"},{"key":"pone.0250262.ref018","doi-asserted-by":"crossref","first-page":"3297","DOI":"10.1093\/cercor\/bhw098","article-title":"Relationship between cortical gyrification, white matter connectivity, and autism spectrum disorder","volume":"26","author":"C Ecker","year":"2016","journal-title":"Cerebral Cortex"},{"key":"pone.0250262.ref019","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1186\/s13229-016-0076-x","article-title":"Cortical morphological markers in children with autism: a structural magnetic resonance imaging study of thickness, area, volume, and gyrification","volume":"7","author":"DY Yang","year":"2016","journal-title":"Molecular Autism"},{"key":"pone.0250262.ref020","doi-asserted-by":"crossref","first-page":"2575","DOI":"10.1093\/cercor\/bhy126","article-title":"Longitudinal study of local gyrification index in young boys with autism spectrum disorder","volume":"29","author":"LE Libero","year":"2019","journal-title":"Cerebral Cortex"},{"key":"pone.0250262.ref021","doi-asserted-by":"crossref","first-page":"1010","DOI":"10.3389\/fnhum.2014.01010","article-title":"Abnormal asymmetry of brain connectivity in schizophrenia","volume":"8","author":"M Ribolsi","year":"2014","journal-title":"Frontiers Human Neuroscience"},{"key":"pone.0250262.ref022","doi-asserted-by":"crossref","first-page":"1861","DOI":"10.2147\/NDT.S145273","article-title":"Cortical gyrification in schizophrenia: current perspectives","volume":"14","author":"Y Matsuda","year":"2018","journal-title":"Neuropsychiatric Disease and Treatment"},{"key":"pone.0250262.ref023","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1111\/j.1600-0447.2008.01286.x","article-title":"Prefrontal gyral folding and its cognitive correlates in bipolar disorder and schizophrenia","volume":"119","author":"AM McIntosh","year":"2009","journal-title":"Acta Psychiatrica Scandinavica"},{"key":"pone.0250262.ref024","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.jad.2015.06.014","article-title":"Prefrontal gyrification in psychotic bipolar I disorder vs. schizophrenia","volume":"185","author":"I Nenadic","year":"2015","journal-title":"Journal of Affective Disorders"},{"key":"pone.0250262.ref025","doi-asserted-by":"crossref","first-page":"13222","DOI":"10.1073\/pnas.1221880110","article-title":"Intrinsic gray-matter connectivity of the brain in adults with autism spectrum disorder","volume":"110","author":"C Ecker","year":"2013","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"key":"pone.0250262.ref026","doi-asserted-by":"crossref","first-page":"750","DOI":"10.3389\/fnhum.2013.00750","article-title":"Decreased frontal gyrification correlates with altered connectivity in children with autism","volume":"7","author":"M Schaer","year":"2013","journal-title":"Frontiers in Human Neuroscience"},{"key":"pone.0250262.ref027","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1016\/j.biopsych.2006.01.015","article-title":"Histological and magnetic resonance imaging assessment of cortical layering and thickness in autism spectrum disorders","volume":"61","author":"JJ Hutsler","year":"2007","journal-title":"Biological Psychiatry"},{"key":"pone.0250262.ref028","doi-asserted-by":"crossref","first-page":"12","DOI":"10.3389\/fchem.2016.00012","article-title":"Prenatal neurogenesis in autism spectrum disorders","volume":"4","author":"G Kaushik","year":"2016","journal-title":"Frontiers in Chemistry"},{"key":"pone.0250262.ref029","doi-asserted-by":"crossref","first-page":"15370","DOI":"10.1038\/srep15370","article-title":"Pathophysiological analyses of cortical malformation using gyrencephalic mammals","volume":"5","author":"K Masuda","year":"2015","journal-title":"Scientific Reports"},{"key":"pone.0250262.ref030","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.29285","article-title":"Gyrification of the cerebral cortex requires FGF signaling in the mammalian brain","volume":"6","author":"N Matsumoto","year":"2017","journal-title":"Elife"},{"key":"pone.0250262.ref031","doi-asserted-by":"crossref","first-page":"2131","DOI":"10.1016\/j.celrep.2017.08.024","article-title":"Folding of the cerebral cortex requires Cdk5 in upper-layer neurons in gyrencephalic mammals","volume":"20","author":"Y Shinmyo","year":"2017","journal-title":"Cell Reports"},{"key":"pone.0250262.ref032","first-page":"256","article-title":"Behavior and neurobehavioral teratology using the ferret","volume":"35","author":"A Rabe","year":"1985","journal-title":"Laboratory Animal Science"},{"key":"pone.0250262.ref033","doi-asserted-by":"crossref","first-page":"6969","DOI":"10.1093\/emboj\/20.24.6969","article-title":"Valproic acid defines a novel class of HDAC inhibitors inducing differentiation of transformed cells","volume":"20","author":"M G\u00f6ttlicher","year":"2001","journal-title":"EMBO Journal"},{"key":"pone.0250262.ref034","doi-asserted-by":"crossref","first-page":"36734","DOI":"10.1074\/jbc.M101287200","article-title":"Histone deacetylase is a direct target of valproic acid, a potent anticonvulsant, mood stabilizer, and teratogen","volume":"276","author":"CJ Phiel","year":"2001","journal-title":"Journal of Biological Chemistry"},{"key":"pone.0250262.ref035","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.brainres.2008.01.055","article-title":"VPA-induced apoptosis and behavioral deficits in neonatal mice","volume":"1203","author":"CL Yochum","year":"2008","journal-title":"Brain Research"},{"key":"pone.0250262.ref036","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/j.ijdevneu.2004.05.004","article-title":"Maternal administration of thalidomide or valproic acid causes abnormal serotonergic neurons in the offspring: implication for pathogenesis of autism","volume":"223","author":"K Miyazaki","year":"2005","journal-title":"International Journal of Developmental Neuroscience"},{"key":"pone.0250262.ref037","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1159\/000341786","article-title":"Effects of rat prenatal exposure to valproic acid on behaviour and neuro-anatomy","volume":"34","author":"R Mychasiuk","year":"2012","journal-title":"Developmental Neuroscience"},{"key":"pone.0250262.ref038","doi-asserted-by":"crossref","first-page":"88","DOI":"10.3389\/fnbeh.2013.00088","article-title":"General developmental health in the VPA-rat model of autism","volume":"7","author":"MR Favre","year":"2013","journal-title":"Frontiers in Behavioral Neuroscience"},{"key":"pone.0250262.ref039","first-page":"497","article-title":"Altered cortical thickness following prenatal sodium valproate exposure","author":"AG Wood","journal-title":"Annals of Clinical and Translational Neurology"},{"key":"pone.0250262.ref040","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1254\/jphs.12025SC","article-title":"Effect of prenatal valproic acid exposure on cortical morphology in female mice","volume":"118","author":"Y Hara","year":"2012","journal-title":"Journal of Pharmacological Sciences"},{"key":"pone.0250262.ref041","doi-asserted-by":"crossref","first-page":"10908","DOI":"10.1523\/JNEUROSCI.0229-16.2016","article-title":"In utero exposure to valproic acid induces neocortical dysgenesis via dysregulation of neural progenitor cell proliferation\/differentiation","volume":"36","author":"K Fujimura","year":"2016","journal-title":"Journal of Neuroscience"},{"key":"pone.0250262.ref042","doi-asserted-by":"crossref","first-page":"27400","DOI":"10.1038\/srep27400","article-title":"Distinct defects in synaptic differentiation of neocortical neurons in response to prenatal valproate exposure","volume":"6","author":"Y Iijima","year":"2016","journal-title":"Scientific Reports"},{"key":"pone.0250262.ref043","doi-asserted-by":"crossref","first-page":"150","DOI":"10.3389\/fnmol.2016.00150","article-title":"Prenatal valproate exposure differentially affects parvalbumin-expressing neurons and related circuits in the cortex and striatum of mice","volume":"9","author":"E Lauber","year":"2016","journal-title":"Frontiers in Molecular Neuroscience"},{"key":"pone.0250262.ref044","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.neulet.2014.07.036","article-title":"Long-term valproic acid exposure increases the number of neocortical neurons in the developing rat brain. A possible new animal model of autism","volume":"580","author":"A Sabers","year":"2014","journal-title":"Neuroscience Letters"},{"key":"pone.0250262.ref045","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.ijdevneu.2016.03.007","article-title":"Effects of developmental alcohol and valproic acid exposure on play behavior of ferrets","volume":"52","author":"TE Krahe","year":"2016","journal-title":"International Journal of Developmental Neuroscience"},{"key":"pone.0250262.ref046","doi-asserted-by":"crossref","first-page":"2039","DOI":"10.1093\/cercor\/bhr284","article-title":"Germinal zones in the developing cerebral cortex of ferret: ontogeny, cell cycle kinetics, and diversity of progenitors","volume":"22","author":"I Reillo","year":"2012","journal-title":"Cerebral Cortex"},{"key":"pone.0250262.ref047","first-page":"18","article-title":"Development and evolution of the human neocortex","volume":"2011146","author":"JH Lui","journal-title":"Cell"},{"key":"pone.0250262.ref048","doi-asserted-by":"crossref","first-page":"24","DOI":"10.3389\/fnhum.2013.00424","article-title":"Conical expansion of the outer subventricular zone and the role of neocortical folding in evolution and development","volume":"7","author":"E Lewitus","year":"2013","journal-title":"Frontiers in Human Neuroscience"},{"key":"pone.0250262.ref049","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1016\/j.neuroimage.2013.06.024","article-title":"MRI-based morphometric characterizations of sexual dimorphism of the cerebrum of ferrets (Mustela putorius)","volume":"83","author":"K Sawada","year":"2013","journal-title":"Neuroimage"},{"key":"pone.0250262.ref050","doi-asserted-by":"crossref","first-page":"55","DOI":"10.3389\/fnana.2015.00055","article-title":"Sexual dimorphism of sulcal morphology of the ferret cerebrum revealed by MRI-based sulcal surface morphometry","volume":"9","author":"K Sawada","year":"2015","journal-title":"Frontiers in Neuroanatomy"},{"key":"pone.0250262.ref051","doi-asserted-by":"crossref","first-page":"1003","DOI":"10.1002\/ar.23369","article-title":"Differential cortical laminar structure revealed by NeuN immunostaining and myeloarchitecture between sulcal and gyral regions independent of sexual dimorphisms in the ferret cerebrum","volume":"299","author":"M Horiuchi-Hirose","year":"2016","journal-title":"Anatomical Record (Hoboken)"},{"key":"pone.0250262.ref052","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.ibror.2019.07.1720","article-title":"Follow-up study of subventricular zone progenitors with multiple rounds of cell division during sulcogyrogenesis in the ferret cerebral cortex","volume":"7","author":"K Sawada","year":"2019","journal-title":"IBRO Reports"},{"key":"pone.0250262.ref053","doi-asserted-by":"crossref","first-page":"2559","DOI":"10.1523\/JNEUROSCI.2645-13.2014","article-title":"Diverse behaviors of outer radial glia in developing ferret and human cortex","volume":"34","author":"CC Gertz","year":"2014","journal-title":"Journal of Neuroscience"},{"key":"pone.0250262.ref054","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1002\/ar.24420","article-title":"Immunohistochemical characterization of postnatal changes in cerebellar cortical cytoarchitectures in ferrets","volume":"304","author":"S Kamiya","year":"2021","journal-title":"Anatomical Record (Hoboken)"},{"key":"pone.0250262.ref055","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1007\/s00429-005-0041-5","article-title":"Developmental mechanics of the primate cerebral cortex","volume":"210","author":"CC Hilgetag","year":"2005","journal-title":"Anatomy and Embryology (Berlin)"},{"key":"pone.0250262.ref056","doi-asserted-by":"crossref","first-page":"620","DOI":"10.1007\/s10517-014-2409-0","article-title":"Administration of histone deacetylase inhibitor during neonatal period changes emotionality of adult male 129Sv mice","volume":"156","author":"OV Burenkova","year":"2014","journal-title":"Bulletin of Experimental Biology and Medicine"},{"key":"pone.0250262.ref057","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.bbr.2018.01.013","article-title":"Inequity aversion is observed in common marmosets but not in marmoset models of autism induced by prenatal exposure to valproic acid","volume":"343","author":"M Yasue","year":"2018","journal-title":"Behavioral Brain Research"},{"key":"pone.0250262.ref058","doi-asserted-by":"crossref","first-page":"1696","DOI":"10.1001\/jama.2013.2270","article-title":"Prenatal valproate exposure and risk of autism spectrum disorders and childhood autism","volume":"309","author":"J Christensen","year":"2013","journal-title":"JAMA"},{"key":"pone.0250262.ref059","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/j.pscychresns.2004.06.001","article-title":"Increased frontal cortical folding in autism: a precliminary MRI study","volume":"131","author":"AY Hardan","year":"2004","journal-title":"Psychiatry Research"},{"key":"pone.0250262.ref060","doi-asserted-by":"crossref","first-page":"16659","DOI":"10.1073\/pnas.0407643101","article-title":"Histone deacetylase inhibition-mediated neuronal differentiation of multipotent adult neural progenitor cells","volume":"101","author":"J Hsieh","year":"2004","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"key":"pone.0250262.ref061","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.neures.2011.09.012","article-title":"Induction of superficial cortical layer neurons from mouse embryonic stem cells by valproic acid","volume":"72","author":"B Juliandi","year":"2012","journal-title":"Neuroscience Research"},{"key":"pone.0250262.ref062","doi-asserted-by":"crossref","first-page":"1280","DOI":"10.1093\/cercor\/12.12.1280","article-title":"Areal organization of the posterior pariet al., cortex of the ferret (Mustela putorius)","volume":"12","author":"PR Manger","year":"2002","journal-title":"Cerebral Cortex"},{"key":"pone.0250262.ref063","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1007\/s00221-009-1843-0","article-title":"Somatosensory and multisensory properties of the medial bank of the ferret rostral suprasylvian sulcus","volume":"196","author":"LP Keniston","year":"2009","journal-title":"Experimental Brain Research"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0250262","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,4,20]],"date-time":"2021-04-20T20:39:35Z","timestamp":1618951175000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0250262"}},"subtitle":[],"editor":[{"given":"Anju","family":"Vasudevan","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2021,4,20]]},"references-count":63,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2021,4,20]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0250262","relation":{},"ISSN":["1932-6203"],"issn-type":[{"value":"1932-6203","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,4,20]]}}}