{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T18:14:49Z","timestamp":1784744089692,"version":"3.55.0"},"reference-count":86,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2021,5,5]],"date-time":"2021-05-05T00:00:00Z","timestamp":1620172800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Manchester Metropolitan University, Cheshire Faculty"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:sec id=\"sec001\">\n<jats:title>Background<\/jats:title>\n<jats:p>Previous work suggest a positive skeletal muscle effect of hormone replacement therapy (HRT) on skeletal muscle characteristics This study aimed to quantify any continued positive effect of HRT even after a sustained hiatus in treatment, controlling for two key muscle modulation hormones: Estradiol (E2) and Tri-iodo-thyronine (T3).<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec002\">\n<jats:title>Method and findings<\/jats:title>\n<jats:p>In 61 untrained women (18-78yrs) stratified as pre-menopausal, post-menopausal without (No_HRT) and post-menopausal with (Used_HRT) HRT history, body composition, physical activity, serum E2 and T3 were assessed by dual energy x-ray absorptiometry, Baecke questionnaire and ELISA. <jats:italic>Gastrocnemius medialis<\/jats:italic> (GM) and <jats:italic>tibialis anterior<\/jats:italic> (TA) electromyographic profiles (mean power frequency (mPowerF)), isometric plantar-flexion (PF) and dorsi-flexion (DF) maximum voluntary contraction (MVC), rate of torque development (RTD), isokinetic MVC and muscle volume, were assessed using surface electromyography, dynamometry and ultrasonography. Muscle quality was quantified as MVC per unit muscle size. E2 and E2:T3 ratio were significantly lower in postmenopausal participants, and were positively correlated with RTD even after controlling for adiposity and\/or age. Pre-menopausal females had greater MVC in 8\/8 PF and 2\/5 DF (23.7\u201398.1%; <jats:italic>P<\/jats:italic>&lt;0.001\u20130.049) strength measures compared to No_HRT, but only 6\/8 PF (17.4\u201342.3%; <jats:italic>P<\/jats:italic>&lt;0.001\u20130.046) strength measures compared to Used_HRT. Notably, Used_HRT had significant higher MVC in 7 PF MVC (30.0%-37.7%; <jats:italic>P<\/jats:italic> = 0.006\u20130.031) measures than No_HRT, while premenopausal and Used_HRT had similar uncorrected muscle size or quality. In addition, this cross-sectional data suggest an annual reduction in GM muscle volume corrected for intra-muscular fat by 1.3% in No_HRT and only 0.5% in Used_HRT.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec003\">\n<jats:title>Conclusion<\/jats:title>\n<jats:p>Even years after cessation of the therapy, a history of HRT is positively associated with negating the expected post-menopausal drop in muscle quantity and quality. Whilst mPowerF did not differ between groups, our work highlights positive associations between RTD against E2 and E2:T3. Notwithstanding our study limitation of single time point for blood sampling, our work is the first to illustrate an HRT attenuation of ageing-related decline in RTD. We infer from these data that high E2, even in the absence of high T3, may help maintain muscle contractile speed and quality. Thus our work is the first to points to markedly larger physiological reserves in women with a past history of HRT.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pone.0250813","type":"journal-article","created":{"date-parts":[[2021,5,5]],"date-time":"2021-05-05T19:54:41Z","timestamp":1620244481000},"page":"e0250813","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":13,"title":["A prolonged hiatus in postmenopausal HRT, does not nullify the therapy\u2019s positive impact on ageing related sarcopenia"],"prefix":"10.1371","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1466-3265","authenticated-orcid":true,"given":"Gladys L.","family":"Onamb\u00e9l\u00e9-Pearson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David 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physiology"},{"issue":"1","key":"pone.0250813.ref002","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/0022-510X(89)90165-2","article-title":"Effects of ageing on enzyme-histochemical, morphometrical and contractile properties of the soleus muscle in the rat","volume":"93","author":"T Ansved","year":"1989","journal-title":"Journal of the neurological sciences"},{"key":"pone.0250813.ref003","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1113\/jphysiol.1987.sp016773","article-title":"Effects of age on contractile and enzyme-histochemical properties of fast- and slow-twitch single motor units in the rat","volume":"392","author":"L Edstrom","year":"1987","journal-title":"The Journal of physiology"},{"issue":"2","key":"pone.0250813.ref004","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1111\/j.1748-1716.1993.tb09493.x","article-title":"An age-related type IIB to IIX myosin heavy chain switching in rat skeletal muscle","volume":"147","author":"L Larsson","year":"1993","journal-title":"Acta physiologica Scandinavica"},{"issue":"1","key":"pone.0250813.ref005","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/0022-510X(86)90143-7","article-title":"Effects of age on enzyme-histochemical fibre spectra and contractile properties of fast- and slow-twitch skeletal muscles in the rat","volume":"76","author":"L Larsson","year":"1986","journal-title":"Journal of the neurological sciences"},{"issue":"4","key":"pone.0250813.ref006","first-page":"647","article-title":"Coupling between skeletal muscle fiber size and capillarization is maintained during healthy aging","volume":"8","author":"Y Barnouin","year":"2017","journal-title":"Journal of cachexia"},{"issue":"1","key":"pone.0250813.ref007","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1046\/j.1365-201x.1998.00329.x","article-title":"Effects of age and gender on shortening velocity and myosin isoforms in single rat muscle fibres","volume":"163","author":"H Degens","year":"1998","journal-title":"Acta physiologica Scandinavica"},{"issue":"2","key":"pone.0250813.ref008","doi-asserted-by":"crossref","first-page":"C638","DOI":"10.1152\/ajpcell.1997.272.2.C638","article-title":"Effects of aging on shortening velocity and myosin isoform composition in single human skeletal muscle cells","volume":"272","author":"L Larsson","year":"1997","journal-title":"The American journal of physiology"},{"issue":"1","key":"pone.0250813.ref009","doi-asserted-by":"crossref","first-page":"e1385","DOI":"10.1371\/journal.pone.0001385","article-title":"Sex-related differences in gene expression in human skeletal muscle","volume":"3","author":"S Welle","year":"2008","journal-title":"PloS one"},{"issue":"1","key":"pone.0250813.ref010","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1152\/physiol.00024.2014","article-title":"Sex-based differences in skeletal muscle kinetics and fiber-type composition.","volume":"30","author":"KM Haizlip","year":"2015","journal-title":"Physiology"},{"key":"pone.0250813.ref011","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.jsbmb.2015.03.003","article-title":"Combined effects of androgen anabolic steroids and physical activity on the hypothalamic-pituitary-gonadal axis","volume":"150","author":"J Hengevoss","year":"2015","journal-title":"The Journal of steroid biochemistry and molecular biology"},{"issue":"6","key":"pone.0250813.ref012","doi-asserted-by":"crossref","first-page":"1555","DOI":"10.1172\/JCI108237","article-title":"Is aromatization of testosterone to estradiol required for inhibition of luteinizing hormone secretion in men?","volume":"56","author":"RJ Santen","year":"1975","journal-title":"The Journal of clinical investigation"},{"issue":"12","key":"pone.0250813.ref013","doi-asserted-by":"crossref","first-page":"2478","DOI":"10.1249\/MSS.0000000000001383","article-title":"Reduced Neuromuscular Performance in Amenorrheic Elite Endurance Athletes.","volume":"49","author":"AB Tornberg","year":"2017","journal-title":"Medicine and science in sports and exercise"},{"issue":"3","key":"pone.0250813.ref014","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1093\/gerona\/62.3.330","article-title":"Loss of appendicular muscle mass and loss of muscle strength in young postmenopausal women","volume":"62","author":"YM Rolland","year":"2007","journal-title":"The journals of gerontology Series A, Biological sciences and medical sciences"},{"issue":"1","key":"pone.0250813.ref015","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1042\/cs0840095","article-title":"Muscle weakness in women occurs at an earlier age than in men, but strength is preserved by hormone replacement therapy","volume":"84","author":"SK Phillips","year":"1993","journal-title":"Clinical science"},{"issue":"5","key":"pone.0250813.ref016","first-page":"2200","article-title":"Angiotensin-I converting enzyme genotype-dependent benefit from hormone replacement therapy in isometric muscle strength and bone mineral density","volume":"86","author":"D Woods","year":"2001","journal-title":"The Journal of clinical endocrinology and metabolism"},{"issue":"4","key":"pone.0250813.ref017","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1093\/humrep\/dem432","article-title":"17-beta-estradiol in relation to age at menarche and adult obesity in premenopausal women","volume":"23","author":"A Emaus","year":"2008","journal-title":"Human reproduction"},{"issue":"5","key":"pone.0250813.ref018","first-page":"611","article-title":"Skeletal muscle fibre plasticity in response to selected environmental and physiological stimuli","volume":"24","author":"A Matsakas","year":"2009","journal-title":"Histology and histopathology"},{"issue":"16","key":"pone.0250813.ref019","first-page":"1174","article-title":"Osteoarthropathies and Myopathies associated with Disorders of the Thyroid Endocrine System","volume":"143","author":"V Nehls","year":"2018","journal-title":"Deutsche medizinische Wochenschrift"},{"issue":"8","key":"pone.0250813.ref020","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1136\/jcp.28.8.659","article-title":"Muscle fibre type changes in hypothyroid myopathy","volume":"28","author":"RO McKeran","year":"1975","journal-title":"Journal of clinical pathology"},{"issue":"1","key":"pone.0250813.ref021","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1007\/s004240050741","article-title":"Gender- and age-related differences in the regulatory influence of thyroid hormone on the contractility and myosin composition of single rat soleus muscle fibres","volume":"437","author":"F Yu","year":"1998","journal-title":"Pflugers Archiv: European journal of physiology"},{"issue":"2","key":"pone.0250813.ref022","doi-asserted-by":"crossref","first-page":"883","DOI":"10.1016\/j.jss.2013.02.029","article-title":"Effect of sex steroids on soleus muscle response in hypocalcemic medium (in vitro).","volume":"185","author":"SS Kokcam","year":"2013","journal-title":"The Journal of surgical research"},{"issue":"5","key":"pone.0250813.ref023","doi-asserted-by":"crossref","first-page":"936","DOI":"10.1093\/ajcn\/36.5.936","article-title":"A short questionnaire for the measurement of habitual physical activity in epidemiological studies","volume":"36","author":"JA Baecke","year":"1982","journal-title":"The American journal of clinical nutrition"},{"issue":"6","key":"pone.0250813.ref024","doi-asserted-by":"crossref","DOI":"10.14814\/phy2.12030","article-title":"Obesity decreases both whole muscle and fascicle strength in young females but only exacerbates the aging-related whole muscle level asthenia.","volume":"2","author":"DJ Tomlinson","year":"2014","journal-title":"Physiological reports."},{"issue":"4","key":"pone.0250813.ref025","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1016\/j.jelekin.2012.04.003","article-title":"Between-day reliability of a method for non-invasive estimation of muscle composition","volume":"22","author":"B Simunic","year":"2012","journal-title":"Journal of electromyography and kinesiology: official journal of the International Society of Electrophysiological Kinesiology"},{"issue":"1","key":"pone.0250813.ref026","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1152\/jappl.1995.79.1.23","article-title":"Effects of muscle fiber type and size on EMG median frequency and conduction velocity","volume":"79","author":"EJ Kupa","year":"1995","journal-title":"Journal of applied physiology"},{"issue":"5","key":"pone.0250813.ref027","doi-asserted-by":"crossref","first-page":"1810","DOI":"10.1152\/jappl.1990.69.5.1810","article-title":"Myoelectric manifestations of fatigue in voluntary and electrically elicited contractions","volume":"69","author":"R Merletti","year":"1990","journal-title":"Journal of applied physiology"},{"issue":"8","key":"pone.0250813.ref028","doi-asserted-by":"crossref","first-page":"1708","DOI":"10.1007\/s10439-012-0531-6","article-title":"A muscle\u2019s force depends on the recruitment patterns of its fibers","volume":"40","author":"JM Wakeling","year":"2012","journal-title":"Annals of biomedical engineering"},{"issue":"1","key":"pone.0250813.ref029","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1186\/s12984-017-0299-x","article-title":"Assessment of the underlying systems involved in standing balance: the additional value of electromyography in system identification and parameter estimation","volume":"14","author":"JH Pasma","year":"2017","journal-title":"Journal of neuroengineering and rehabilitation"},{"issue":"6","key":"pone.0250813.ref030","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1001\/archneur.1968.00480060061008","article-title":"Orderly recruitment of muscle action potentials","volume":"19","author":"CB Olson","year":"1968","journal-title":"Archives of neurology"},{"issue":"6","key":"pone.0250813.ref031","doi-asserted-by":"crossref","first-page":"1088","DOI":"10.1016\/j.jelekin.2010.06.004","article-title":"Wavelet based correlation and coherence analysis reveals frequency dependent motor unit conduction velocity of the abductor pollicis brevis muscle","volume":"20","author":"V von Tscharner","year":"2010","journal-title":"Journal of electromyography and kinesiology: official journal of the International Society of Electrophysiological Kinesiology"},{"issue":"Pt 14","key":"pone.0250813.ref032","doi-asserted-by":"crossref","first-page":"2519","DOI":"10.1242\/jeb.01042","article-title":"Spectral properties of myoelectric signals from different motor units in the leg extensor muscles","volume":"207","author":"JM Wakeling","year":"2004","journal-title":"The Journal of experimental biology"},{"issue":"6","key":"pone.0250813.ref033","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1113\/expphysiol.1998.sp004164","article-title":"Differences in human antagonistic ankle dorsiflexor coactivation between legs; can they explain the moment deficit in the weaker plantarflexor leg","volume":"83","author":"CN Maganaris","year":"1998","journal-title":"Experimental physiology"},{"issue":"6","key":"pone.0250813.ref034","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1111\/joa.12248","article-title":"The impact of obesity on skeletal muscle architecture in untrained young vs. old women","volume":"225","author":"DJ Tomlinson","year":"2014","journal-title":"Journal of anatomy"},{"issue":"1","key":"pone.0250813.ref035","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1007\/s00421-003-0961-9","article-title":"Ultrasonographic assessment of human skeletal muscle size","volume":"91","author":"ND Reeves","year":"2004","journal-title":"European journal of applied physiology"},{"key":"pone.0250813.ref036","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1186\/1471-2474-15-209","article-title":"Age-associated differences in triceps surae muscle composition and strength\u2014an MRI-based cross-sectional comparison of contractile, adipose and connective tissue","volume":"15","author":"R Csapo","year":"2014","journal-title":"BMC musculoskeletal disorders."},{"issue":"5632","key":"pone.0250813.ref037","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1038\/270074a0","article-title":"Thyroidal trophic influence on skeletal muscle myosin","volume":"270","author":"D Ianuzzo","year":"1977","journal-title":"Nature"},{"issue":"1","key":"pone.0250813.ref038","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1097\/gme.0b013e3181e3aab1","article-title":"Effect of hormone therapy on lean body mass, falls, and fractures: 6-year results from the Women\u2019s Health Initiative hormone trials","volume":"18","author":"JW Bea","year":"2011","journal-title":"Menopause"},{"issue":"4","key":"pone.0250813.ref039","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1042\/CS19980318","article-title":"Hormone replacement therapy increases isometric muscle strength of adductor pollicis in post-menopausal women","volume":"96","author":"DA Skelton","year":"1999","journal-title":"Clinical science"},{"issue":"1","key":"pone.0250813.ref040","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1111\/j.1748-1716.2006.01597.x","article-title":"Oestrogen status in relation to the early training responses in human thumb adductor muscles","volume":"188","author":"GN Onambele","year":"2006","journal-title":"Acta physiologica"},{"issue":"4","key":"pone.0250813.ref041","doi-asserted-by":"crossref","first-page":"1387","DOI":"10.1152\/japplphysiol.01305.2006","article-title":"Estradiol replacement reverses ovariectomy-induced muscle contractile and myosin dysfunction in mature female mice","volume":"102","author":"AL Moran","year":"2007","journal-title":"Journal of applied physiology"},{"issue":"2","key":"pone.0250813.ref042","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1152\/japplphysiol.01029.2005","article-title":"Removal of ovarian hormones from mature mice detrimentally affects muscle contractile function and myosin structural distribution","volume":"100","author":"AL Moran","year":"2006","journal-title":"Journal of applied physiology"},{"issue":"4","key":"pone.0250813.ref043","doi-asserted-by":"crossref","first-page":"e10164","DOI":"10.1371\/journal.pone.0010164","article-title":"Estrogen regulates estrogen receptors and antioxidant gene expression in mouse skeletal muscle","volume":"5","author":"KA Baltgalvis","year":"2010","journal-title":"PloS one"},{"issue":"4","key":"pone.0250813.ref044","doi-asserted-by":"crossref","first-page":"E854","DOI":"10.1152\/ajpendo.90696.2008","article-title":"Estrogen receptor-alpha and -beta and aromatase knockout effects on lower limb muscle mass and contractile function in female mice","volume":"296","author":"M Brown","year":"2009","journal-title":"American journal of physiology Endocrinology and metabolism"},{"issue":"11","key":"pone.0250813.ref045","doi-asserted-by":"crossref","first-page":"4613","DOI":"10.1210\/endo.138.11.5496","article-title":"Tissue distribution and quantitative analysis of estrogen receptor-alpha (ERalpha) and estrogen receptor-beta (ERbeta) messenger ribonucleic acid in the wild-type and ERalpha-knockout mouse","volume":"138","author":"JF Couse","year":"1997","journal-title":"Endocrinology"},{"issue":"3","key":"pone.0250813.ref046","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1249\/01.MSS.0000053654.14410.78","article-title":"Estrogen receptor alpha mRNA in human skeletal muscles","volume":"35","author":"S Lemoine","year":"2003","journal-title":"Medicine and science in sports and exercise"},{"issue":"4","key":"pone.0250813.ref047","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1046\/j.0001-6772.2003.01186.x","article-title":"Oestrogen receptor beta is expressed in adult human skeletal muscle both at the mRNA and protein level","volume":"179","author":"A Wiik","year":"2003","journal-title":"Acta physiologica Scandinavica"},{"issue":"4","key":"pone.0250813.ref048","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1042\/CS20000264","article-title":"Follow-up study of the benefits of hormone replacement therapy on isometric muscle strength of adductor pollicis in postmenopausal women","volume":"100","author":"NG Onambele","year":"2001","journal-title":"Clinical science"},{"issue":"9","key":"pone.0250813.ref049","doi-asserted-by":"crossref","first-page":"941","DOI":"10.1055\/s-0035-1557815","article-title":"High Estradiol Levels During Postmenopause\u2014Pitfalls in Laboratory Analysis","volume":"75","author":"I Mebes","year":"2015","journal-title":"Geburtshilfe und Frauenheilkunde"},{"issue":"4","key":"pone.0250813.ref050","doi-asserted-by":"crossref","first-page":"1332","DOI":"10.1177\/0300060517706630","article-title":"Low plasma PDGF-BB levels are associated with estradiol in postmenopausal osteoporosis: PDGF-BB mediated by estradiol in women","volume":"45","author":"L Tang","year":"2017","journal-title":"The Journal of international medical research"},{"issue":"9\u201310","key":"pone.0250813.ref051","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1016\/j.steroids.2007.12.027","article-title":"17beta-estradiol signaling in skeletal muscle cells and its relationship to apoptosis","volume":"73","author":"R Boland","year":"2008","journal-title":"Steroids"},{"issue":"5","key":"pone.0250813.ref052","doi-asserted-by":"crossref","first-page":"1909","DOI":"10.1096\/fj.11-194779","article-title":"Selective estrogen receptor-beta activation stimulates skeletal muscle growth and regeneration","volume":"26","author":"M Velders","year":"2012","journal-title":"FASEB journal: official publication of the Federation of American Societies for Experimental Biology"},{"issue":"2","key":"pone.0250813.ref053","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1677\/erc.0.0100331","article-title":"Interactions between estrogen and insulin-like growth factor signaling pathways in human breast tumor cells","volume":"10","author":"IH Hamelers","year":"2003","journal-title":"Endocrine-related cancer"},{"issue":"3","key":"pone.0250813.ref054","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1111\/j.1748-1716.2009.01997.x","article-title":"Oestradiol and SERM treatments influence oestrogen receptor coregulator gene expression in human skeletal muscle cells","volume":"197","author":"CM Dieli-Conwright","year":"2009","journal-title":"Acta physiologica"},{"key":"pone.0250813.ref055","first-page":"560","volume":"9","author":"FF Bloise","year":"2018","journal-title":"Thyroid Hormones Play Role in Sarcopenia and Myopathies. Frontiers in physiology"},{"issue":"1","key":"pone.0250813.ref056","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1756-6614-5-1","article-title":"Gender-specific regulation of response to thyroid hormone in aging","volume":"5","author":"S Suzuki","year":"2012","journal-title":"Thyroid research"},{"issue":"4","key":"pone.0250813.ref057","doi-asserted-by":"crossref","first-page":"e13003","DOI":"10.1111\/apha.13003","article-title":"Thyroid hormone upregulates MDM2 in rat type I fibre: Implications for skeletal muscle mass regulation","volume":"222","author":"GV Ramos","year":"2018","journal-title":"Acta physiologica"},{"issue":"3","key":"pone.0250813.ref058","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1002\/jcp.20273","article-title":"Differential effects of thyroid hormones on energy metabolism of rat slow- and fast-twitch muscles","volume":"203","author":"L Bahi","year":"2005","journal-title":"Journal of cellular physiology"},{"key":"pone.0250813.ref059","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1113\/jphysiol.1983.sp014835","article-title":"The effect of hyperthyroidism on capillarity and oxidative capacity in rat soleus and gastrocnemius muscles","volume":"342","author":"LA Capo","year":"1983","journal-title":"The Journal of physiology"},{"issue":"3","key":"pone.0250813.ref060","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1042\/bj1940685","article-title":"Effect of experimental hyperthyroidism on skeletal-muscle proteolysis","volume":"194","author":"WJ Carter","year":"1981","journal-title":"The Biochemical journal"},{"issue":"2","key":"pone.0250813.ref061","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1101\/gr.207702","article-title":"In vivo regulation of human skeletal muscle gene expression by thyroid hormone","volume":"12","author":"K Clement","year":"2002","journal-title":"Genome research"},{"issue":"3","key":"pone.0250813.ref062","doi-asserted-by":"crossref","first-page":"1050","DOI":"10.1152\/japplphysiol.01186.2004","article-title":"In vivo physiological cross-sectional area and specific force are reduced in the gastrocnemius of elderly men","volume":"99","author":"CI Morse","year":"2005","journal-title":"Journal of applied physiology"},{"issue":"4854","key":"pone.0250813.ref063","doi-asserted-by":"crossref","first-page":"889","DOI":"10.1126\/science.3283939","article-title":"The steroid and thyroid hormone receptor superfamily","volume":"240","author":"RM Evans","year":"1988","journal-title":"Science"},{"issue":"22","key":"pone.0250813.ref064","doi-asserted-by":"crossref","first-page":"12587","DOI":"10.1073\/pnas.93.22.12587","article-title":"Estrogen and thyroid hormone interaction on regulation of gene expression","volume":"93","author":"YS Zhu","year":"1996","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"1\u20132","key":"pone.0250813.ref065","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/S0960-0760(97)00009-5","article-title":"Up-regulation of the estrogen receptor by triiodothyronine in rat pituitary cell lines","volume":"61","author":"N Fujimoto","year":"1997","journal-title":"J Steroid Biochem"},{"issue":"4","key":"pone.0250813.ref066","first-page":"413","article-title":"Histochemical Muscle Fiber Types of Autopsied Human Gastrocnemius, Soleus, Peroneus longus and Tibialis anterior Muscles","volume":"20","author":"JC Yang","year":"1986","journal-title":"The Korean Journal of Pathology"},{"issue":"1","key":"pone.0250813.ref067","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1152\/japplphysiol.91518.2008","article-title":"Postmenopausal hormone replacement therapy modifies skeletal muscle composition and function: a study with monozygotic twin pairs","volume":"107","author":"PH Ronkainen","year":"2009","journal-title":"Journal of applied 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Method for the preparation and analysis of cross-sections of whole tibialis anterior.","volume":"15","author":"KB Henriksson-Larsen","year":"1983","journal-title":"The Histochemical journal"},{"issue":"1","key":"pone.0250813.ref075","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1007\/BF00421761","article-title":"Maximal isometric strength and fiber type composition in power and endurance athletes","volume":"44","author":"PM Clarkson","year":"1980","journal-title":"European journal of applied physiology and occupational physiology"},{"issue":"1","key":"pone.0250813.ref076","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1177\/028418519303400104","article-title":"MR relaxation times and fiber type predominance of the psoas and multifidus muscle. 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