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Some reports have suggested a link between<jats:italic>SPATS1<\/jats:italic>underexpression\/mutation and human pathologies such as male infertility and testicular cancer. Given the absence of functional studies, we generated a<jats:italic>Spats1<\/jats:italic>loss-of-function mouse model using CRISPR\/Cas9 technology. The phenotypic analysis showed no overt phenotype in<jats:italic>Spats1<\/jats:italic><jats:sup><jats:italic>-\/-<\/jats:italic><\/jats:sup>mice, with both males and females being fertile. Flow cytometry and histological analyses did not show differences in the testicular content and histology between WT and knockout mice. Moreover, no significant differences in sperm concentration, motility, and morphology, were observed between WT and KO mice. These results were obtained both for young adults and for aged animals. Besides, although an involvement of SPATS1 in the Wnt signaling pathway has been suggested, we did not detect changes in the expression levels of typical Wnt pathway-target genes in mutant individuals. Thus, albeit<jats:italic>Spats1<\/jats:italic>alteration might be a risk factor for male testicular health, we hereby show that this gene is not individually essential for male fertility and spermatogenesis in mouse.<\/jats:p>","DOI":"10.1371\/journal.pone.0251028","type":"journal-article","created":{"date-parts":[[2021,5,4]],"date-time":"2021-05-04T19:30:19Z","timestamp":1620156619000},"page":"e0251028","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":7,"title":["SPATS1 (spermatogenesis-associated, serine-rich 1) is not essential for spermatogenesis and fertility in mouse"],"prefix":"10.1371","volume":"16","author":[{"given":"Carlos A.","family":"Capoano","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis 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