{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T07:37:28Z","timestamp":1791877048032,"version":"4.3.4"},"reference-count":33,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2021,5,24]],"date-time":"2021-05-24T00:00:00Z","timestamp":1621814400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Erasmus Medical Center and Erasmus University Rotterdam"},{"DOI":"10.13039\/501100001826","name":"Netherlands Organization for the Health Research and Development","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001826","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Research Institute for Diseases in the Elderly"},{"name":"Ministry of Education, Culture and Science, the Ministry for Health, Welfare and Sports, the European Commission"},{"name":"Municipality of Rotterdam"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:sec id=\"sec001\">\n<jats:title>Background<\/jats:title>\n<jats:p>Seborrheic dermatitis (SD) is a chronic inflammatory skin disease with a multifactorial aetiology. <jats:italic>Malassezia<\/jats:italic> yeasts have been associated with the disease but the role of bacterial composition in SD has not been thoroughly investigated.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec002\">\n<jats:title>Objectives<\/jats:title>\n<jats:p>To profile the bacterial microbiome of SD patients and compare this with the microbiome of individuals with no inflammatory skin disease (controls).<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec003\">\n<jats:title>Methods<\/jats:title>\n<jats:p>This was a cross sectional study embedded in a population-based study. Skin swabs were taken from naso-labial fold from patients with seborrheic dermatitis (lesional skin: n = 22; non-lesional skin SD: n = 75) and controls (n = 465). Sample collection began in 2016 at the research facility and is still ongoing. Shannon and Chao1 \u03b1- diversity metrics were calculated per group. Associations between the microbiome composition of cases and controls was calculated using multivariate statistics (permANOVA) and univariate statistics.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec004\">\n<jats:title>Results<\/jats:title>\n<jats:p>We found an increased \u03b1-diversity between SD lesional cases versus controls (Shannon diversity: Kruskal-Wallis rank sum: Chi-squared: 19.06; global p-value = 7.7x10<jats:sup>-5<\/jats:sup>). Multivariate statistical analysis showed significant associations in microbiome composition when comparing lesional SD skin to controls (p-value = 0.03;R<jats:sup>2<\/jats:sup> = 0.1%). Seven out of 13 amplicon sequence variants (ASVs) that were significantly different between controls and lesional cases were members of the genus <jats:italic>Staphylococcus<\/jats:italic>, most of which showed increased composition in lesional cases, and were closely related to <jats:italic>S<\/jats:italic>. <jats:italic>capitis S<\/jats:italic>. <jats:italic>caprae<\/jats:italic> and <jats:italic>S<\/jats:italic>. e<jats:italic>pidermidis<\/jats:italic>.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec005\">\n<jats:title>Conclusion<\/jats:title>\n<jats:p>Microbiome composition differs in patients with seborrheic dermatitis and individuals without diseases. Differences were mainly found in the genus <jats:italic>Staphylococcus<\/jats:italic>.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pone.0251136","type":"journal-article","created":{"date-parts":[[2021,5,24]],"date-time":"2021-05-24T17:26:06Z","timestamp":1621877166000},"page":"e0251136","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":32,"title":["Composition of cutaneous bacterial microbiome in seborrheic dermatitis patients: A cross-sectional study"],"prefix":"10.1371","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2361-7228","authenticated-orcid":true,"given":"Martijn G. 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