{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T18:30:00Z","timestamp":1793298600538,"version":"4.5.0"},"reference-count":47,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2021,9,16]],"date-time":"2021-09-16T00:00:00Z","timestamp":1631750400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000069","name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","doi-asserted-by":"publisher","award":["AR065949"],"award-info":[{"award-number":["AR065949"]}],"id":[{"id":"10.13039\/100000069","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000069","name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","doi-asserted-by":"publisher","award":["AR070340"],"award-info":[{"award-number":["AR070340"]}],"id":[{"id":"10.13039\/100000069","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Platelet-rich plasma (PRP) is a widely used autologous treatment for tendon injuries in clinics. Platelets (PLTs) are a major source of high mobility group box1 (HMGB1) that is gaining attention as a chemoattractant that can recruit stem cells to the wound area to enhance healing of injured tissues; however, the contribution of PLT HMGB1 in wounded tendon healing remains unexplored. This study investigated the effect of PLT HMGB1 within PRP on tendon healing using PLT HMGB1 knockout (KO) and GFP mice. A window defect was created in the patellar tendons of both groups of mice, and wounds were treated with either saline, PRP isolated from PLT HMGB1-KO mice, or PRP isolated from GFP mice. Seven days post-treatment, animals were sacrificed and analyzed by gross inspection, histology, and immunostaining for characteristic signs of tendon healing and repair. Our results showed that in comparison to mice treated with PRP from PLT HMGB1-KO mice, wounds treated with PRP from GFP mice healed faster and exhibited a better organization in tendon structure. Mice treated with PRP from PLT HMGB1-KO mice produced tendon tissue with large premature wound areas and low cell densities. However, wounds of PLT HMGB1-KO mice showed better healing with PRP from HMGB1-KO mice compared to saline treatment. Moreover, wounds treated with PRP from GFP mice had increased extracellular HMGB1, decreased CD68, increased stem cell markers CD146 and CD73, and increased collagen III protein expression levels compared to those treated with PRP from PLT HMGB1-KO mice. Thus, PLT HMGB1 within PRP plays an important role in tendon wound healing by decreasing inflammation, increasing local HMGB1 levels, and recruiting stem cells to the wound area in the tendon. Our findings also suggest that the efficacy of PRP treatment for tendon injuries in clinics may depend on PLT HMGB1 within PRP preparations.<\/jats:p>","DOI":"10.1371\/journal.pone.0251166","type":"journal-article","created":{"date-parts":[[2021,9,16]],"date-time":"2021-09-16T13:38:51Z","timestamp":1631799531000},"page":"e0251166","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":14,"title":["Platelet HMGB1 in Platelet-Rich Plasma (PRP) promotes tendon wound healing"],"prefix":"10.1371","volume":"16","author":[{"given":"Jianying","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3346-2175","authenticated-orcid":true,"given":"Feng","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tyler","family":"Augi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7529-0220","authenticated-orcid":true,"given":"Kelly M.","family":"Williamson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kentaro","family":"Onishi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MaCalus V.","family":"Hogan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew D.","family":"Neal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7279-0679","authenticated-orcid":true,"given":"James H.-C.","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2021,9,16]]},"reference":[{"issue":"10","key":"pone.0251166.ref001","doi-asserted-by":"crossref","first-page":"1415","DOI":"10.1517\/14712598.2010.514603","article-title":"Tendon healing and platelet-rich plasma therapies","volume":"10","author":"I Andia","year":"2010","journal-title":"Expert Opin Biol Ther"},{"key":"pone.0251166.ref002","first-page":"1","article-title":"Advances with platelet rich plasma therapies for tendon regeneration","author":"I Andia","year":"2018","journal-title":"Expert Opin Biol Ther"},{"issue":"4","key":"pone.0251166.ref003","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1007\/s00167-010-1306-y","article-title":"Platelet-rich plasma (PRP) to treat sports injuries: evidence to support its use","volume":"19","author":"E Kon","year":"2011","journal-title":"Knee Surg Sports Traumatol Arthrosc"},{"issue":"5","key":"pone.0251166.ref004","doi-asserted-by":"crossref","first-page":"379","DOI":"10.3113\/FAI.2012.0379","article-title":"Platelet rich plasma treatment for chronic Achilles tendinosis","volume":"33","author":"RR Monto","year":"2012","journal-title":"Foot Ankle Int"},{"issue":"3","key":"pone.0251166.ref005","doi-asserted-by":"crossref","first-page":"610","DOI":"10.1177\/0363546513518416","article-title":"Platelet-rich plasma as a treatment for patellar tendinopathy: a double-blind, randomized controlled trial","volume":"42","author":"JL Dragoo","year":"2014","journal-title":"Am J Sports Med"},{"issue":"2","key":"pone.0251166.ref006","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1001\/jama.2009.1986","article-title":"Platelet-rich plasma injection for chronic Achilles tendinopathy: a randomized controlled trial. 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