{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T14:05:21Z","timestamp":1740146721227,"version":"3.37.3"},"reference-count":42,"publisher":"Public Library of Science (PLoS)","issue":"5","license":[{"start":{"date-parts":[[2021,5,10]],"date-time":"2021-05-10T00:00:00Z","timestamp":1620604800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000289","name":"Cancer Research UK","doi-asserted-by":"publisher","award":["FC001155"],"award-info":[{"award-number":["FC001155"]}],"id":[{"id":"10.13039\/501100000289","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004440","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["FC001155"],"award-info":[{"award-number":["FC001155"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009187","name":"Medical Research Foundation","doi-asserted-by":"publisher","award":["FC001155"],"award-info":[{"award-number":["FC001155"]}],"id":[{"id":"10.13039\/501100009187","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Chl1 is a member of the XPD family of 5\u2019-3\u2019 DNA helicases, which perform a variety of roles in genome maintenance and transmission. They possess a variety of unique structural features, including the presence of a highly variable, partially-ordered insertion in the helicase domain 1. Chl1 has been shown to be required for chromosome segregation in yeast due to its role in the formation of persistent chromosome cohesion during S-phase. Here we present structural and biochemical data to show that Chl1 has the same overall domain organisation as other members of the XPD family, but with some conformational alterations. We also present data suggesting the insert domain in Chl1 regulates its DNA binding.<\/jats:p>","DOI":"10.1371\/journal.pone.0251261","type":"journal-article","created":{"date-parts":[[2021,5,10]],"date-time":"2021-05-10T18:52:38Z","timestamp":1620672758000},"page":"e0251261","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":0,"title":["Structural characterisation of the Chaetomium thermophilum Chl1 helicase"],"prefix":"10.1371","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2599-0333","authenticated-orcid":true,"given":"Zuzana","family":"Hod\u00e1kov\u00e1","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3791-2447","authenticated-orcid":true,"given":"Andrea","family":"Nans","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simone","family":"Kunzelmann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shahid","family":"Mehmood","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6763-3852","authenticated-orcid":true,"given":"Ian","family":"Taylor","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3527-6619","authenticated-orcid":true,"given":"Frank","family":"Uhlmann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Cherepanov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5716-7715","authenticated-orcid":true,"given":"Martin R.","family":"Singleton","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2021,5,10]]},"reference":[{"issue":"7","key":"pone.0251261.ref001","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1038\/nrm.2016.30","article-title":"SMC complexes: from DNA to chromosomes","volume":"17","author":"F Uhlmann","year":"2016","journal-title":"Nat Rev Mol Cell Biol"},{"key":"pone.0251261.ref002","doi-asserted-by":"crossref","DOI":"10.1038\/nature07098","article-title":"The cohesin ring concatenates sister DNA molecules","volume":"454","author":"CH Haering","year":"2008","journal-title":"Nature"},{"key":"pone.0251261.ref003","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/S1097-2765(00)80420-7","article-title":"Cohesin\u2019s Binding to Chromosomes Depends on a Separate Complex Consisting of Scc2 and Scc4 Proteins","volume":"5","author":"R Ciosk","year":"2000","journal-title":"Mol Cell"},{"issue":"July","key":"pone.0251261.ref004","doi-asserted-by":"crossref","first-page":"566","DOI":"10.1126\/science.1157880","article-title":"A Molecular Determinant for the Establishment of Sister Chromatid Cohesion","volume":"321","author":"E Unal","year":"2008","journal-title":"Science (80-)"},{"issue":"July","key":"pone.0251261.ref005","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1126\/science.1157774","article-title":"Eco1-Dependent Cohesin Sister Chromatid Cohesion","volume":"321","author":"TR Ben-Shahar","year":"2008","journal-title":"Science (80-)."},{"key":"pone.0251261.ref006","doi-asserted-by":"crossref","first-page":"1417","DOI":"10.1534\/genetics.107.072876","article-title":"Genetic Dissection of Parallel Sister-Chromatid Cohesion Pathways","volume":"176","author":"H Xu","year":"2007","journal-title":"Genetics"},{"issue":"April","key":"pone.0251261.ref007","doi-asserted-by":"crossref","first-page":"1736","DOI":"10.1091\/mbc.e03-08-0619","article-title":"Identification of Protein Complexes Required for Efficient Sister Chromatid Cohesion","volume":"15","author":"ML Mayer","year":"2004","journal-title":"Mol Biol Cell"},{"issue":"9","key":"pone.0251261.ref008","doi-asserted-by":"crossref","first-page":"3144","DOI":"10.1128\/MCB.21.9.3144-3158.2001","article-title":"Saccharomyces cerevisiae CTF18 and CTF4 Are Required for Sister Chromatid Cohesion","volume":"21","author":"JS Hanna","year":"2001","journal-title":"Mol Cell Biol"},{"key":"pone.0251261.ref009","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1534\/genetics.166.1.33","article-title":"Chl1p, a DNA Helicase-like Protein in Budding Yeast, Functions in Sister-Chromatid Cohesion","volume":"166","author":"V Skibbens R","year":"2004","journal-title":"Genetics"},{"issue":"3","key":"pone.0251261.ref010","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1093\/genetics\/78.3.843","article-title":"Bisexual mating behavior in a diploid of Saccharomyces cerevisiae: evidence for genetically controlled non random chromosome loss during vegetative growth","volume":"78","author":"JE Haber","year":"1974","journal-title":"Genetics"},{"issue":"3","key":"pone.0251261.ref011","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1016\/j.molcel.2016.05.036","article-title":"Ctf4 Links DNA Replication with Sister Chromatid Cohesion Establishment by Recruiting the Chl1 Helicase to the Replisome","volume":"63","author":"CP Samora","year":"2016","journal-title":"Mol Cell"},{"issue":"1\u20132","key":"pone.0251261.ref012","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1007\/s00412-013-0396-y","article-title":"An Eco1-independent sister chromatid cohesion establishment pathway in S. cerevisiae","volume":"122","author":"V Borges","year":"2013","journal-title":"Chromosoma"},{"key":"pone.0251261.ref013","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1038\/nature13234","article-title":"A Ctf4 trimer couples the CMG helicase to DNA polymerase a in the eukaryotic replisome","volume":"510","author":"AC Simon","year":"2014","journal-title":"Nature"},{"key":"pone.0251261.ref014","first-page":"1","article-title":"Timeless couples G\u2010quadruplex detection with processing by DDX 11 helicase during DNA replication","author":"LK Lerner","year":"2020","journal-title":"EMBO J"},{"key":"pone.0251261.ref015","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.molcel.2020.04.012","article-title":"Cryo-EM Structure of the Fork Protection Complex Bound to CMG at a Replication Fork","volume":"78","author":"D Baretic","year":"2020","journal-title":"Mol Cell"},{"key":"pone.0251261.ref016","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.pgen.1007622","article-title":"Interaction of the Warsaw breakage syndrome DNA helicase DDX11 with the replication fork-protection factor Timeless promotes sister chromatid cohesion.","volume":"14","author":"G Cortone","year":"2018","journal-title":"PLoS Genet"},{"issue":"42","key":"pone.0251261.ref017","doi-asserted-by":"crossref","first-page":"67934","DOI":"10.18632\/oncotarget.11982","article-title":"Chromatin determinants of the inner-centromere rely on replication factors with functions that impart cohesion","volume":"7","author":"T Abe","year":"2016","journal-title":"Oncotarget"},{"issue":"2","key":"pone.0251261.ref018","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1093\/nar\/gkv1112","article-title":"Perna R Di, Jr RMB, Pisani FM. Tim \/ Timeless, a member of the replication fork protection complex, operates with the Warsaw breakage syndrome DNA helicase DDX11 in the same fork recovery pathway","volume":"44","author":"F Cali","year":"2016","journal-title":"Nucleic Acids Res"},{"key":"pone.0251261.ref019","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1016\/j.ajhg.2010.01.008","article-title":"Warsaw Breakage Syndrome, a Cohesinopathy Associated with Mutations in the XPD Helicase Family Member DDX11 \/ ChlR1","volume":"86","author":"P Van Der Lelij","year":"2010","journal-title":"Am J Hum Genet"},{"issue":"1","key":"pone.0251261.ref020","doi-asserted-by":"crossref","first-page":"e0220348","DOI":"10.1371\/journal.pone.0220348","article-title":"Non-redundant roles in sister chromatid cohesion of the DNA helicase DDX11 and the SMC3 acetyl transferases ESCO1 and ESCO2","volume":"15","author":"A Faramarz","year":"2020","journal-title":"PLoS One"},{"key":"pone.0251261.ref021","doi-asserted-by":"crossref","first-page":"801","DOI":"10.1016\/j.cell.2008.04.029","article-title":"Structure of the DNA Repair Helicase XPD","volume":"133","author":"H Liu","year":"2008","journal-title":"Cell"},{"issue":"5","key":"pone.0251261.ref022","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1016\/j.cell.2008.04.030","article-title":"XPD Helicase Structures and Activities: Insights into the Cancer and Aging Phenotypes from XPD Mutations","volume":"133","author":"L Fan","year":"2008","journal-title":"Cell"},{"issue":"6","key":"pone.0251261.ref023","doi-asserted-by":"crossref","first-page":"801","DOI":"10.1016\/j.molcel.2006.07.019","article-title":"The DNA Repair Helicases XPD and FancJ Have Essential Iron-Sulfur Domains","volume":"23","author":"J Rudolf","year":"2006","journal-title":"Mol Cell"},{"key":"pone.0251261.ref024","doi-asserted-by":"crossref","first-page":"1","DOI":"10.7554\/eLife.42400","article-title":"DNA translocation mechanism of an XPD family helicase","volume":"7","author":"K Cheng","year":"2018","journal-title":"Elife"},{"issue":"2","key":"pone.0251261.ref025","doi-asserted-by":"crossref","first-page":"107545","DOI":"10.1016\/j.jsb.2020.107545","article-title":"Mitigating local over-fitting during single particle reconstruction with SIDESPLITTER","volume":"211","author":"K Ramlaul","year":"2020","journal-title":"J Struct Biol"},{"issue":"7","key":"pone.0251261.ref026","doi-asserted-by":"crossref","first-page":"1209","DOI":"10.1007\/s00018-008-8580-6","article-title":"Welcome the Family of FANCJ-like Helicases to the Block of Genome Stability Maintenance Proteins","volume":"66","author":"Y Wu","year":"2009","journal-title":"Cell Mol Life Sci"},{"issue":"6","key":"pone.0251261.ref027","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pbio.0060149","article-title":"Crystal Structure of the FeS Cluster\u2013Containing Nucleotide Excision Repair Helicase XPD","volume":"6","author":"SC Wolski","year":"2008","journal-title":"PLOS Biol"},{"issue":"6","key":"pone.0251261.ref028","doi-asserted-by":"crossref","first-page":"2806","DOI":"10.1093\/nar\/gkw102","article-title":"Mechanism of DNA loading by the DNA repair helicase XPD","volume":"44","author":"D Constantinescu-Aruxandei","year":"2016","journal-title":"Nucleic Acids Res"},{"issue":"2","key":"pone.0251261.ref029","doi-asserted-by":"crossref","first-page":"494","DOI":"10.1038\/emboj.2011.374","article-title":"Functional and structural studies of the nucleotide excision repair helicase XPD suggest a polarity for DNA translocation","volume":"31","author":"J Kuper","year":"2011","journal-title":"EMBO J"},{"issue":"9","key":"pone.0251261.ref030","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pbio.1001954","article-title":"In TFIIH, XPD Helicase Is Exclusively Devoted to DNA Repair","volume":"12","author":"J Kuper","year":"2014","journal-title":"PLoS Biol"},{"issue":"1","key":"pone.0251261.ref031","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.molcel.2008.04.024","article-title":"Nucleotide Excision Repair Driven by the Dissociation of CAK from TFIIH","volume":"31","author":"F Coin","year":"2008","journal-title":"Mol Cell"},{"issue":"7672","key":"pone.0251261.ref032","doi-asserted-by":"crossref","first-page":"414","DOI":"10.1038\/nature23903","article-title":"The cryo-electron microscopy structure of human transcription factor IIH","volume":"549","author":"BJ Greber","year":"2017","journal-title":"Nature"},{"issue":"7679","key":"pone.0251261.ref033","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1038\/nature24282","article-title":"Structures of transcription pre-initiation complex with TFIIH and Mediator","volume":"551","author":"S Schilbach","year":"2017","journal-title":"Nature"},{"issue":"1","key":"pone.0251261.ref034","first-page":"1","article-title":"Structural basis of TFIIH activation for nucleotide excision repair. Nat Commun","volume":"10","author":"G Kokic","year":"2019","journal-title":"Internet]"},{"issue":"13","key":"pone.0251261.ref035","doi-asserted-by":"crossref","first-page":"3238","DOI":"10.1038\/sj.emboj.7601754","article-title":"The FANCJ\/MutL\u03b1 interaction is required for correction of the cross-link response in FA-J cells","volume":"26","author":"M Peng","year":"2007","journal-title":"EMBO J"},{"issue":"4","key":"pone.0251261.ref036","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1038\/nmeth.4193","article-title":"MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy","volume":"14","author":"SQ Zheng","year":"2017","journal-title":"Nat Methods"},{"issue":"1","key":"pone.0251261.ref037","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jsb.2015.11.003","article-title":"Gctf: Real-time CTF determination and correction","volume":"193","author":"K Zhang","year":"2016","journal-title":"J Struct Biol"},{"issue":"1","key":"pone.0251261.ref038","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.jsb.2006.05.009","article-title":"EMAN2: An extensible image processing suite for electron microscopy","volume":"157","author":"G Tang","year":"2007","journal-title":"J Struct Biol"},{"issue":"3","key":"pone.0251261.ref039","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1038\/nmeth.4169","article-title":"CryoSPARC: Algorithms for rapid unsupervised cryo-EM structure determination","volume":"14","author":"A Punjani","year":"2017","journal-title":"Nat Methods"},{"key":"pone.0251261.ref040","doi-asserted-by":"crossref","first-page":"1","DOI":"10.7554\/eLife.42166","article-title":"New tools for automated high-resolution cryo-EM structure determination in RELION-3","volume":"7","author":"J Zivanov","year":"2018","journal-title":"Elife"},{"issue":"3","key":"pone.0251261.ref041","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1016\/j.jsb.2012.09.006","article-title":"RELION: Implementation of a Bayesian approach to cryo-EM structure determination","volume":"180","author":"SHW Scheres","year":"2012","journal-title":"J Struct Biol"},{"issue":"8","key":"pone.0251261.ref042","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1038\/nmeth.4347","article-title":"Addressing preferred specimen orientation in single-particle cryo-EMthrough tilting","volume":"14","author":"YZ Tan","year":"2017","journal-title":"Nat Methods"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0251261","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,5,10]],"date-time":"2021-05-10T18:53:37Z","timestamp":1620672817000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0251261"}},"subtitle":[],"editor":[{"given":"Arthur J.","family":"Lustig","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2021,5,10]]},"references-count":42,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2021,5,10]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0251261","relation":{},"ISSN":["1932-6203"],"issn-type":[{"type":"electronic","value":"1932-6203"}],"subject":[],"published":{"date-parts":[[2021,5,10]]}}}