{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T18:29:49Z","timestamp":1783535389210,"version":"3.55.0"},"reference-count":19,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2021,6,2]],"date-time":"2021-06-02T00:00:00Z","timestamp":1622592000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["75N93019C00018"],"award-info":[{"award-number":["75N93019C00018"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Growth in open-source hardware designs combined with the decreasing cost of high-quality 3D printers have supported a resurgence of in-house custom lab equipment development. Herein, we describe a low-cost (&lt; $400), open-source CO<jats:sub>2<\/jats:sub>incubator. The system is comprised of a Raspberry Pi computer connected to a 3D printer controller board that has controls for a CO<jats:sub>2<\/jats:sub>sensor, solenoid valve, heater, and thermistors. CO<jats:sub>2<\/jats:sub>is supplied through the sublimation of dry ice stored inside a thermos to create a sustained 5% CO<jats:sub>2<\/jats:sub>supply. The unit is controlled via G-Code commands sent by the Raspberry Pi to the controller board. In addition, we built a custom software application for remote control and used the open-source Grafana dashboard for remote monitoring. Our data show that we can maintain consistent CO<jats:sub>2<\/jats:sub>and temperature levels for over three days without manual interruption. The results from our culture plates and real-time PCR indicate that our incubator performed equally well when compared to a much more expensive commercial CO<jats:sub>2<\/jats:sub>incubator. We have also demonstrated that the antibiotic susceptibility assay can be performed in this low-cost CO<jats:sub>2<\/jats:sub>incubator. Our work also indicates that the system can be connected to incubator chambers of various chamber volumes.<\/jats:p>","DOI":"10.1371\/journal.pone.0251812","type":"journal-article","created":{"date-parts":[[2021,6,2]],"date-time":"2021-06-02T17:37:15Z","timestamp":1622655435000},"page":"e0251812","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":17,"title":["PrintrLab incubator: A portable and low-cost CO2 incubator based on an open-source 3D printer architecture"],"prefix":"10.1371","volume":"16","author":[{"given":"Arunkumar","family":"Arumugam","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cole","family":"Markham","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saurabh 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