{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T03:01:24Z","timestamp":1787022084141,"version":"build-2736575974"},"reference-count":51,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2021,6,8]],"date-time":"2021-06-08T00:00:00Z","timestamp":1623110400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Abu Dhabi Agriculture and Food Safety Authority"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>\n                    Caseous lymphadenitis (CLA) or pseudotuberculosis is a chronic zoonotic bacterial disease caused by\n                    <jats:italic>Corynebacterium pseudotuberculosis<\/jats:italic>\n                    , which affects livestock and humans. This study aimed to describe the pathology, bacteriology and confirm the identity of the pathogen by 16S rRNA gene sequencing in Camelus dromedarius. A total of 12 camels with suspected CLA in three regions of Abu Dhabi Emirate (Abu Dhabi, Al Ain and Al Dhafra), United Arab Emirate (UAE) were subjected to clinical and postmortem examinations from January 2015 to December 2020. Clinically, camels were emaciated and showed the presence of external caseous abscesses suggestive of CLA. Postmortem examination showed multiple abscesses of variable sizes with caseous material encapsulated by fibrous tissue in the liver, lungs, muscle, and lymph nodes. Following clinical and postmortem examination, blood, pus and different tissue samples were collected for subsequent analysis. Histopathological examination of all organs stained with Hematoxylin and Eosin (H&amp;E) indicated a central caseo-necrotic core that was admixed with bacterial colonies and infiltration of chronic inflammatory cells, surrounded by a pyogenic membrane, and an outer fibrous connective tissue capsule. Bacterial culture identified the isolates of\n                    <jats:italic>Corynebacterium pseudotuberculosis<\/jats:italic>\n                    biotype ovis strain, and these isolates were shown to be sensitive to all antibiotics tested (penicillin, ampicillin, Co-trimoxazole, enrofloxacin and tetracycline). Moreover, the identity of the isolates was confirmed by partial sequencing of the 16S rRNA gene which showed a 100% identity to\n                    <jats:italic>Corynebacterium pseudotuberculosis<\/jats:italic>\n                    . Phylogenetic analysis based on 16S rRNA gene sequence clearly differentiates\n                    <jats:italic>Corynebacterium pseudotuberculosis<\/jats:italic>\n                    from other species of\n                    <jats:italic>Corynebacterium<\/jats:italic>\n                    . Briefly, this study provided the basic information for infection of\n                    <jats:italic>Corynebacterium pseudotuberculosis<\/jats:italic>\n                    in Camels and will help in controlling of this pathogen in the region.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0252893","type":"journal-article","created":{"date-parts":[[2021,6,8]],"date-time":"2021-06-08T13:54:39Z","timestamp":1623160479000},"page":"e0252893","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":14,"title":["Pathology, bacteriology and molecular studies on caseous lymphadenitis in Camelus dromedarius in the Emirate of Abu Dhabi, UAE, 2015-2020"],"prefix":"10.1371","volume":"16","author":[{"given":"Abdelnasir Mohammed Adam","family":"Terab","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ghada El Derdiri","family":"Abdel Wahab","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4723-1909","authenticated-orcid":true,"given":"Hassan Zackaria Ali","family":"Ishag","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nasereldien Altaib Hussein","family":"Khalil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"El Tigani Ahmed","family":"El Tigani-Asil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farouk Mohamed","family":"Hashem","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9156-1361","authenticated-orcid":true,"given":"Abdelmalik Ibrahim","family":"Khalafalla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asma Abdi Mohamed","family":"Shah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salama Suhail Mohammed","family":"Al Muhairi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2021,6,8]]},"reference":[{"key":"pone.0252893.ref001","first-page":"202","volume-title":"Jubb, Kennedy and Palmer\u2019s pathology of domestic animals","author":"VEO Ted Valli","year":"2016","edition":"6"},{"issue":"2","key":"pone.0252893.ref002","first-page":"39","article-title":"Studies on lymphoid tissue abscesses in camels (Camelus dromedarius) slaughtered at Nyala Slaughterhouse, South Darfur State, Sudan","volume":"4","author":"MA Aljameel","year":"2013","journal-title":"U of K J Vet Med & Anim Prod"},{"key":"pone.0252893.ref003","unstructured":"Zidan K H, Mazloum K, Saran MA, Hatem ME. Abscesses in dromedary camels, sheep and goats etiology and pathology. 1st International scientific conference of Pathology Department, Faculty of Veterinary Medicine, Cairo University, Egypt, April 25\u201327 2013, 2013;47:59."},{"issue":"1","key":"pone.0252893.ref004","first-page":"1","article-title":"Caseous lymphadenitis (Pseudotuberculosis) in camelids: a review","volume":"3","author":"U Wernery","year":"2016","journal-title":"Austin J Vet Sci & Anim Husb"},{"issue":"1\u20132","key":"pone.0252893.ref005","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.smallrumres.2007.12.025","article-title":"Caseous lymphadenitis","volume":"76","author":"MC Fontaine","year":"2008","journal-title":"Small Ruminant Res"},{"issue":"2","key":"pone.0252893.ref006","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1093\/clinids\/24.2.185","article-title":"Human lymphadenitis due to Corynebacterium pseudotuberculosis: report of ten cases from Australia and review","volume":"24","author":"MM Peel","year":"1997","journal-title":"Clin Infect Dis"},{"key":"pone.0252893.ref007","first-page":"005","article-title":"Corynebacterium pseudotuberculosis: immunological responses in animal models and zoonotic potential","volume":"S4","author":"BL Bastos","year":"2012","journal-title":"J Clin & Cellular Immunol"},{"issue":"20","key":"pone.0252893.ref008","doi-asserted-by":"crossref","first-page":"5718","DOI":"10.1128\/JB.01373-12","article-title":"Whole-genome sequence of Corynebacterium pseudotuberculosis strain Cp162, isolated from camel","volume":"194","author":"SS Hassan","year":"2012","journal-title":"J Bacteriol"},{"issue":"05","key":"pone.0252893.ref009","doi-asserted-by":"crossref","first-page":"506","DOI":"10.3855\/jidc.7023","article-title":"An outbreak of dermatophilosis and caseous lymphadenitis mixed infection in camels (Camelus dromedaries) in Jordan","volume":"10","author":"YH Tarazi","year":"2016","journal-title":"J Infect Dev Ctries"},{"issue":"3","key":"pone.0252893.ref010","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1007\/s11250-007-9077-2","article-title":"Isolation of nitrate-reductase positive Corynebacterium pseudotuberculosis from dromedary camels","volume":"40","author":"MT Tejedor-Junco","year":"2008","journal-title":"Trop Anim Health Prod"},{"issue":"6","key":"pone.0252893.ref011","doi-asserted-by":"crossref","first-page":"e98758","DOI":"10.1371\/journal.pone.0098758","article-title":"Evaluation of ERIC-PCR as genotyping method for Corynebacterium pseudotuberculosis isolates","volume":"9","author":"EM Dorneles","year":"2014","journal-title":"PLoS One"},{"issue":"1","key":"pone.0252893.ref012","doi-asserted-by":"crossref","first-page":"e53818","DOI":"10.1371\/journal.pone.0053818","article-title":"The pan-genome of the animal pathogen Corynebacterium pseudotuberculosis reveals differences in genome plasticity between the biovar ovis and equi strains","volume":"8","author":"SC Soares","year":"2013","journal-title":"PLoS One"},{"issue":"2","key":"pone.0252893.ref013","first-page":"126","article-title":"Caseous lymphadenitis caused by Corynebacterium ulcerans in the dromedary camel","volume":"41","author":"MT Tejedor","year":"2000","journal-title":"Can Vet J"},{"issue":"3","key":"pone.0252893.ref014","first-page":"313","article-title":"First observation of camel (Camelus dromedarius) lymphadenitis in Libya. A case report","volume":"47","author":"AM Moustafa","year":"1994","journal-title":"Rev Elev Med Vet Pays Trop"},{"key":"pone.0252893.ref015","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/B978-1-4377-2353-3.10007-1","volume-title":"Sheep and goat medicine","author":"PJ Plummer","year":"2012","edition":"2"},{"issue":"1","key":"pone.0252893.ref016","first-page":"21","article-title":"Caseous lymphadenitis (Pseudotuberculosis) in camelids","volume":"19","author":"U Wernery","year":"2012","journal-title":"J Camel Pract Res"},{"issue":"2","key":"pone.0252893.ref017","first-page":"68","article-title":"Corynebacterium pseudotuberculosis infection (caseous lymphadenitis) in camels (Camelus dromedarius) in Jordan","volume":"3","author":"AD Hawari","year":"2008","journal-title":"Am J Anim & Vet Sci"},{"issue":"2","key":"pone.0252893.ref018","doi-asserted-by":"crossref","first-page":"52","DOI":"10.5455\/ajvs.282343","article-title":"Caseous lymphadenitis in Sudanese and Somalian 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Lane","year":"1991"},{"issue":"1","key":"pone.0252893.ref034","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1023\/A:1000669317571","article-title":"Application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology","volume":"73","author":"G Muyzer","year":"1998","journal-title":"Antonie van Leeuwenhoek"},{"issue":"8","key":"pone.0252893.ref035","doi-asserted-by":"crossref","first-page":"1166","DOI":"10.1093\/bioinformatics\/bts091","article-title":"Unipro UGENE: a unified bioinformatics toolkit","volume":"28","author":"K Okonechnikov","year":"2012","journal-title":"Bioinformatics"},{"key":"pone.0252893.ref036","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.smallrumres.2018.09.015","article-title":"Isolation, antibiotic resistance, virulence traits and phylogenetic analysis of Corynebacterium pseudotuberculosis from goats in southwestern China","volume":"168","author":"H 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States","volume":"74","author":"MK Ashfaq","year":"1979","journal-title":"Vet Med Small Anim Clin"},{"issue":"3","key":"pone.0252893.ref047","first-page":"1129.1","article-title":"The epidemiology and pathophysiology of caseous lymphadenitis: A review","volume":"5","author":"AY Osman","year":"2018","journal-title":"J Vet Med Res"},{"issue":"1","key":"pone.0252893.ref048","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/S0034-5288(18)33322-8","article-title":"Immunological events in the popliteal lymph node of sheep following injection of live or killed Corynebacterium ovis into an afferent popliteal lymphatic duct","volume":"22","author":"AJ Husband","year":"1977","journal-title":"Res Vet Sci"},{"issue":"5","key":"pone.0252893.ref049","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1023\/B:TROP.0000035012.63821.12","article-title":"Pseudotuberculosis in dromedary camels in the Canary Islands","volume":"36","author":"MT 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