{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T04:28:35Z","timestamp":1779164915810,"version":"3.51.4"},"reference-count":73,"publisher":"Public Library of Science (PLoS)","issue":"11","license":[{"start":{"date-parts":[[2021,11,18]],"date-time":"2021-11-18T00:00:00Z","timestamp":1637193600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"TTUHSC Office of Research"},{"name":"Laura W. Bush Institute for Women\u2019s Health & UMC Health System"},{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"publisher","award":["R01\/R56NS077114"],"award-info":[{"award-number":["R01\/R56NS077114"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>The Proton-Coupled Folate Transporter (PCFT) is a transmembrane transport protein that controls the absorption of dietary folates in the small intestine. PCFT also mediates uptake of chemotherapeutically used antifolates into tumor cells. PCFT has been identified within lipid rafts observed in phospholipid bilayers of plasma membranes, a micro environment that is altered in tumor cells. The present study aimed at investigating the impact of different lipids within Lipid-protein nanodiscs (LPNs), discoidal lipid structures stabilized by membrane scaffold proteins, to yield soluble PCFT expression in an <jats:italic>E<\/jats:italic>. <jats:italic>coli<\/jats:italic> lysate-based cell-free transcription\/translation system. In the absence of detergents or lipids, we observed PCFT quantitatively as precipitate in this system. We then explored the ability of LPNs to support solubilized PCFT expression when present during in-vitro translation. LPNs consisted of either dimyristoyl phosphatidylcholine (DMPC), palmitoyl-oleoyl phosphatidylcholine (POPC), or dimyristoyl phosphatidylglycerol (DMPG). While POPC did not lead to soluble PCFT expression, both DMPG and DMPC supported PCFT translation directly into LPNs, the latter in a concentration dependent manner. The results obtained through this study provide insights into the lipid preferences of PCFT. Membrane-embedded or solubilized PCFT will enable further studies with diverse biophysical approaches to enhance the understanding of the structure and molecular mechanism of folate transport through PCFT.<\/jats:p>","DOI":"10.1371\/journal.pone.0253184","type":"journal-article","created":{"date-parts":[[2021,11,18]],"date-time":"2021-11-18T18:29:09Z","timestamp":1637260149000},"page":"e0253184","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":5,"title":["Impact of nanodisc lipid composition on cell-free expression of proton-coupled folate transporter"],"prefix":"10.1371","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7361-6066","authenticated-orcid":true,"given":"Hoa Quynh","family":"Do","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carla M.","family":"Bassil","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth I.","family":"Andersen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1325-9700","authenticated-orcid":true,"given":"Michaela","family":"Jansen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2021,11,18]]},"reference":[{"issue":"2","key":"pone.0253184.ref001","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1042\/bst0270053","article-title":"Dihydropteroate synthase: an old drug target revisited","volume":"27","author":"HG Vinnicombe","year":"1999","journal-title":"Biochem Soc Trans"},{"issue":"8","key":"pone.0253184.ref002","doi-asserted-by":"crossref","first-page":"1067","DOI":"10.1016\/j.addr.2004.01.001","article-title":"Distribution, functionality and gene regulation of folate receptor isoforms: implications in targeted therapy","volume":"56","author":"H Elnakat","year":"2004","journal-title":"Adv Drug Deliv Rev"},{"key":"pone.0253184.ref003","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/S0083-6729(03)01012-4","article-title":"Membrane transport of folates","volume":"66","author":"LH Matherly","year":"2003","journal-title":"Vitam Horm"},{"issue":"1","key":"pone.0253184.ref004","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1007\/s10555-007-9046-2","article-title":"Human reduced folate carrier: translation of basic biology to cancer etiology and therapy","volume":"26","author":"LH Matherly","year":"2007","journal-title":"Cancer Metastasis Rev"},{"issue":"5","key":"pone.0253184.ref005","doi-asserted-by":"crossref","first-page":"917","DOI":"10.1016\/j.cell.2006.09.041","article-title":"Identification of an intestinal folate transporter and the molecular basis for hereditary folate malabsorption","volume":"127","author":"AD Qiu","year":"2006","journal-title":"Cell"},{"issue":"11","key":"pone.0253184.ref006","doi-asserted-by":"crossref","first-page":"5299","DOI":"10.1167\/iovs.07-0288","article-title":"Cloning and functional characterization of the proton-coupled electrogenic folate transporter and analysis of its expression in retinal cell types","volume":"48","author":"NS Umapathy","year":"2007","journal-title":"Investigative Ophthalmology & Visual Science"},{"issue":"1","key":"pone.0253184.ref007","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1007\/s10555-007-9047-1","article-title":"The molecular identity and characterization of a Proton-coupled Folate Transporter\u2014PCFT; 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