{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T23:25:00Z","timestamp":1790810700417,"version":"4.1.0"},"reference-count":56,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2021,6,11]],"date-time":"2021-06-11T00:00:00Z","timestamp":1623369600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Data on the distribution and genotype of <jats:italic>Cryptosporidium<\/jats:italic> species is limited in Ethiopia. This study examined the presence and genetic diversity of <jats:italic>Cryptosporidium<\/jats:italic> species circulating in Ethiopian human population. Stool samples collected from patients who visited rural (n = 94) and urban (n = 93) health centers in Wurgissa and Hawassa district, respectively, were examined for the presence of <jats:italic>Cryptosporidium<\/jats:italic> spp. using microscopy, nested PCR and real-time PCR. To detect infection with PCR, analysis of <jats:italic>18S<\/jats:italic> ribosomal RNA was performed. Subtyping was performed by sequencing a fragment of <jats:italic>GP60<\/jats:italic> gene. The overall prevalence of infection was 46% (n = 86) by microscope and PCR. When 48 (out of 86) PCR positive samples were genotyped, two species were identified: <jats:italic>C<\/jats:italic>. <jats:italic>parvum<\/jats:italic> (n = 40) and <jats:italic>C<\/jats:italic>. <jats:italic>hominis<\/jats:italic> (n = 8). When 15 of the 40 <jats:italic>C<\/jats:italic>. <jats:italic>parvum<\/jats:italic> isolates were subtyped, zoonotic subtypes of IIaA14G1R1 (n = 1), IIaA15G2R1 (n = 1), IIaA16G1R1 (n = 2), IIaA16G3R1 (n = 2), IIaA17G1R1 (n = 1), IIaA19G1R1 (n = 1), IIaA20G1R1 (n = 3), IIaA22G1R1 (n = 1), IIaA22G2R1 (n = 1), IIdA23G1 (n = 1) and IIdA24G1 (n = 1) were identified. When 6 of the 8 <jats:italic>C<\/jats:italic>. <jats:italic>hominis<\/jats:italic> isolates were subtyped, subtypes IaA20 (n = 5), and IdA21(n = 1) were identified. This study suggests that <jats:italic>C<\/jats:italic>. <jats:italic>parvum<\/jats:italic> and <jats:italic>C<\/jats:italic>. <jats:italic>hominis<\/jats:italic> are causes of cryptosporidiosis in human in the Wurgissa district and Hawassa in Ethiopia. Zoonotic transmission might be the main route of transmission.<\/jats:p>","DOI":"10.1371\/journal.pone.0253186","type":"journal-article","created":{"date-parts":[[2021,6,11]],"date-time":"2021-06-11T17:39:29Z","timestamp":1623433169000},"page":"e0253186","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":17,"title":["Molecular characterization of Cryptosporidium spp. from humans in Ethiopia"],"prefix":"10.1371","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2958-5471","authenticated-orcid":true,"given":"Ambachew W.","family":"Hailu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abraham","family":"Degarege","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haileeyesus","family":"Adamu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3418-2597","authenticated-orcid":true,"given":"Damien","family":"Costa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3679-7616","authenticated-orcid":true,"given":"Venceslas","family":"Villier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3170-4057","authenticated-orcid":true,"given":"Abdelmounaim","family":"Mouhajir","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Loic","family":"Favennec","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Romy","family":"Razakandrainibe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beyene","family":"Petros","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2021,6,11]]},"reference":[{"key":"pone.0253186.ref001","first-page":"555","article-title":"Burden of disease from cryptosporidiosis Opin","volume":"25","author":"DA Shirley","year":"2012","journal-title":"Infect. 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