{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T01:16:27Z","timestamp":1791335787704,"version":"4.3.1"},"reference-count":27,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2021,9,7]],"date-time":"2021-09-07T00:00:00Z","timestamp":1630972800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000030","name":"Centers for Disease Control and Prevention","doi-asserted-by":"publisher","award":["5U01CK000538-03"],"award-info":[{"award-number":["5U01CK000538-03"]}],"id":[{"id":"10.13039\/100000030","id-type":"DOI","asserted-by":"publisher"}]},{"name":"University of Utah Immunology, Inflammation, & Infectious Disease Initiative","award":["26798 Seed Grant"],"award-info":[{"award-number":["26798 Seed Grant"]}]},{"name":"Becton, Dickinson, and Company"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Surveillance testing for infectious disease is an important tool to combat disease transmission at the population level. During the SARS-CoV-2 pandemic, RT-PCR tests have been considered the gold standard due to their high sensitivity and specificity. However, RT-PCR tests for SARS-CoV-2 have been shown to return positive results when performed to individuals who are past the infectious stage of the disease. Meanwhile, antigen-based tests are often treated as a less accurate substitute for RT-PCR, however, new evidence suggests they may better reflect infectiousness. Consequently, the two test types may each be most optimally deployed in different settings. Here, we present an epidemiological model with surveillance testing and coordinated isolation in two congregate living settings (a nursing home and a university dormitory system) that considers test metrics with respect to viral culture, a proxy for infectiousness. Simulations show that antigen-based surveillance testing coupled with isolation greatly reduces disease burden and carries a lower economic cost than RT-PCR-based strategies. Antigen and RT-PCR tests perform different functions toward the goal of reducing infectious disease burden and should be used accordingly.<\/jats:p>","DOI":"10.1371\/journal.pone.0253407","type":"journal-article","created":{"date-parts":[[2021,9,7]],"date-time":"2021-09-07T16:04:25Z","timestamp":1631030665000},"page":"e0253407","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":15,"title":["Comparison of antigen- and RT-PCR-based testing strategies for detection of SARS-CoV-2 in two high-exposure settings"],"prefix":"10.1371","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9371-2466","authenticated-orcid":true,"given":"Jay","family":"Love","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Megan T.","family":"Wimmer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Damon J. A.","family":"Toth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arthi","family":"Chandran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dilip","family":"Makhija","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles K.","family":"Cooper","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew H.","family":"Samore","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lindsay T.","family":"Keegan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2021,9,7]]},"reference":[{"key":"pone.0253407.ref001","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1016\/S1473-3099(20)30120-1","article-title":"An interactive web-based dashboard to track COVID-19 in real time","volume":"20","author":"E Dong","year":"2020","journal-title":"Lancet Infect Dis"},{"key":"pone.0253407.ref002","first-page":"2020","article-title":"SARS-CoV-2 Vaccines and the Growing Threat of Viral Variants","volume":"4399","author":"JP Moore","year":"2021","journal-title":"JAMA\u2014J Am Med Assoc"},{"key":"pone.0253407.ref003","doi-asserted-by":"crossref","first-page":"160","DOI":"10.3390\/vaccines9020160","article-title":"COVID-19 Vaccine Hesitancy Worldwide: A Concise Systematic Review of Vaccine Acceptance Rates","volume":"9","author":"M Sallam","year":"2021","journal-title":"Vaccines"},{"key":"pone.0253407.ref004","article-title":"Continued need for non-pharmaceutical interventions after COVID-19 vaccination in long-term- care facilities","author":"J Love","year":"2021","journal-title":"medRxiv"},{"issue":"e83","key":"pone.0253407.ref005","article-title":"False Negative Tests for SARS-CoV-2 Infection\u2014Challenges and Implications","volume":"383","author":"S. Woloshin","year":"2020","journal-title":"New England Journal of Medicine"},{"key":"pone.0253407.ref006","unstructured":"CDC (2021a). Overview of Testing for SARS-CoV-2 (COVID-19). 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