{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:03:30Z","timestamp":1792789410254,"version":"4.5.0"},"reference-count":48,"publisher":"Public Library of Science (PLoS)","issue":"6","license":[{"start":{"date-parts":[[2021,6,24]],"date-time":"2021-06-24T00:00:00Z","timestamp":1624492800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Key R&D Program of China","award":["2018YFD0501200"],"award-info":[{"award-number":["2018YFD0501200"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32002320"],"award-info":[{"award-number":["32002320"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Guangdong Basic and Applied Basic Research Foundation","award":["2020A1515010116"],"award-info":[{"award-number":["2020A1515010116"]}]},{"name":"Guangdong Basic and Applied Basic Research Foundation","award":["2019A1515110785"],"award-info":[{"award-number":["2019A1515110785"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>\n                    Porcine epidemic diarrhea virus (\n                    <jats:italic>PEDV<\/jats:italic>\n                    ), a leading cause of piglet diarrhea outbreaks, poses a significant danger to the swine industry. The aim of this study was to investigate the epidemic characteristics of\n                    <jats:italic>PEDV<\/jats:italic>\n                    that was circulating in Guangdong province, one of China\u2019s major pig producing provinces. Clinical samples were collected from eight pig farms in Guangdong province between 2018 and 2019 and tested for the major porcine enteric pathogens, including\n                    <jats:italic>PEDV<\/jats:italic>\n                    , transmissible gastroenteritis virus (\n                    <jats:italic>TGEV)<\/jats:italic>\n                    , Swine enteric coronavirus (\n                    <jats:italic>SeCoV)<\/jats:italic>\n                    , Swine acute diarrhea syndrome coronavirus (\n                    <jats:italic>SADS-CoV)<\/jats:italic>\n                    , porcine deltacoronavirus (\n                    <jats:italic>PDCoV<\/jats:italic>\n                    ), and porcine rotavirus (\n                    <jats:italic>RV<\/jats:italic>\n                    ). As a result, only\n                    <jats:italic>PEDV<\/jats:italic>\n                    and\n                    <jats:italic>RV<\/jats:italic>\n                    were detected at a rate of 47.0% (16\/34) and 18.6% (8\/34), respectively. Coinfectoin with\n                    <jats:italic>PEDV<\/jats:italic>\n                    and RV occurred at a rate of\n                    <jats:italic>PEDV<\/jats:italic>\n                    12.5% (2\/16). Subsequently, the full-length S gene sequences of 13\n                    <jats:italic>PEDV<\/jats:italic>\n                    strains were obtained, and phylogenetic analysis suggested the presence of GII-c group PEDV strains in this region (non-S-INDEL). Two novel common amino acid insertions (\n                    <jats:sup>55<\/jats:sup>\n                    T\/IG\n                    <jats:sup>56<\/jats:sup>\n                    and 551L) and one novel glycosylation site (1199\n                    <jats:sup>G+<\/jats:sup>\n                    ) were detected when the CV777 and ZJ08 vaccine strains were compared. Furthermore, intragroup recombination events in the\n                    <jats:italic>S<\/jats:italic>\n                    gene regions 51\u2013548 and 2478\u20134208 were observed in the\n                    <jats:italic>PEDV<\/jats:italic>\n                    strains studied. In summary, the observations provide current information on the incidence of viral agents causing swine diarrhea in southern China and detailed the genetic characteristics and evolutionary history of the dominant\n                    <jats:italic>PEDV<\/jats:italic>\n                    field strains. Our findings will aid in the development of an updated vaccine for the prevention and control of\n                    <jats:italic>PEDV<\/jats:italic>\n                    variant strains.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0253622","type":"journal-article","created":{"date-parts":[[2021,6,24]],"date-time":"2021-06-24T13:37:37Z","timestamp":1624541857000},"page":"e0253622","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":34,"title":["Genetic characterization and phylogenetic analysis of porcine epidemic diarrhea virus in Guangdong, China, between 2018 and 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