{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T04:07:21Z","timestamp":1768882041619,"version":"3.49.0"},"reference-count":54,"publisher":"Public Library of Science (PLoS)","issue":"10","license":[{"start":{"date-parts":[[2021,10,7]],"date-time":"2021-10-07T00:00:00Z","timestamp":1633564800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81772245"],"award-info":[{"award-number":["81772245"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Research indicates that <jats:italic>Staphylococcus aureus<\/jats:italic> colonization in the elderly with predisposing risks is associated with subsequent infection. However, the molecular epidemiology and risk factors for <jats:italic>S<\/jats:italic>. <jats:italic>aureus<\/jats:italic> colonization among residents and staff in nursing homes (NHs) in China remain unclear. A multicenter study was conducted in three NHs in Shanghai between September 2019 and October 2019. We explored the prevalence, molecular epidemiology, and risk factors for <jats:italic>S<\/jats:italic>. <jats:italic>aureus<\/jats:italic> colonization. All <jats:italic>S<\/jats:italic>. <jats:italic>aureus<\/jats:italic> isolates were characterized based on antimicrobial resistance, virulence genes, multilocus sequence typing (MLST), staphylococcus protein A (<jats:italic>spa<\/jats:italic>) typing, and staphylococcal cassette chromosome <jats:italic>mec<\/jats:italic> (SCC<jats:italic>mec<\/jats:italic>) typing. NH records were examined for potential risk factors for <jats:italic>S<\/jats:italic>. <jats:italic>aureus<\/jats:italic> colonization. <jats:italic>S<\/jats:italic>. <jats:italic>aureus<\/jats:italic> and methicillin-resistant <jats:italic>S<\/jats:italic>. <jats:italic>aureus<\/jats:italic> (MRSA) isolates were detected in 109 (100 residents and 9 staff, 19.8%, 109\/551) and 28 (24 residents and 4 staff, 5.1%, 28\/551) subjects among 496 residents and 55 staff screened, respectively. Compared to methicillin-susceptible <jats:italic>S<\/jats:italic>. <jats:italic>aureus<\/jats:italic> isolates, all 30 MRSA isolates had higher resistance rates to most antibiotics except minocycline, rifampicin, linezolid, vancomycin, and teicoplanin. Sequence type (ST) 1 (21.3%) was the most common sequence type, and t127 (20.5%) was the most common <jats:italic>spa<\/jats:italic> type among 122 <jats:italic>S<\/jats:italic>. <jats:italic>aureus<\/jats:italic> isolates. SCC<jats:italic>mec<\/jats:italic> type I (70%) was the dominant clone among all MRSA isolates. CC1 (26\/122, 21.3%) was the predominant complex clone (CC), followed by CC398 (25\/122, 20.5%), CC5 (20\/122, 16.4%) and CC188 (18\/122, 14.8%). Female sex (OR, 1.70; 95% CI, 1.04\u20132.79; <jats:italic>P<\/jats:italic> = 0.036) and invasive devices (OR, 2.19; 95% CI, 1.26\u20133.81; <jats:italic>P<\/jats:italic> = 0.006) were independently associated with <jats:italic>S<\/jats:italic>. <jats:italic>aureus<\/jats:italic> colonization.<\/jats:p>","DOI":"10.1371\/journal.pone.0253858","type":"journal-article","created":{"date-parts":[[2021,10,8]],"date-time":"2021-10-08T14:56:57Z","timestamp":1633705017000},"page":"e0253858","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":6,"title":["Molecular characteristics and risk factor analysis of Staphylococcus aureus colonization put insight into CC1 colonization in three nursing homes in 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