{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T12:05:41Z","timestamp":1788869141273,"version":"build-2803163510"},"reference-count":26,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2021,7,1]],"date-time":"2021-07-01T00:00:00Z","timestamp":1625097600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Key Research and Development Program of China","award":["2017YFD0501000"],"award-info":[{"award-number":["2017YFD0501000"]}]},{"DOI":"10.13039\/501100012476","name":"Fundamental Research Funds for Central Universities","doi-asserted-by":"publisher","award":["2662019PY061"],"award-info":[{"award-number":["2662019PY061"]}],"id":[{"id":"10.13039\/501100012476","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Enterococci have the dual characteristics of being opportunistic pathogens and promising probiotics. The isolation from patients of CDC PNS-E2, a newly described <jats:italic>Enterococcus<\/jats:italic> species <jats:italic>Enterococcus sanguinicola<\/jats:italic>, may pose potential hazards. <jats:italic>Enterococcus thailandicus<\/jats:italic> from fermented sausage is a senior subjective synonym of <jats:italic>E<\/jats:italic>. <jats:italic>sanguinicola<\/jats:italic>. In this study, <jats:italic>Enterococcus thailandicus<\/jats:italic> TC1 was first isolated in healthy pigs in Tongcheng, China and identified by phenotypic analysis and 16S rRNA-based techniques. To evaluate the strain safety, an approach including virulence factors, antibiotic resistance, and animal experiments was adopted. The results show that <jats:italic>cylA<\/jats:italic>, <jats:italic>gelE<\/jats:italic>, <jats:italic>esp<\/jats:italic>, <jats:italic>agg<\/jats:italic>, <jats:italic>ace<\/jats:italic>, <jats:italic>efaAfm<\/jats:italic>, <jats:italic>efaAfs<\/jats:italic>, <jats:italic>ptsD<\/jats:italic> genes were undetected, and that the strain was sensitive or poorly resistant to some clinically relevant antibiotics. However, the isolated strain demonstrated \u03b2-hemolytic activity in rabbit blood agar plates. Analysis of animal experiments revealed that the isolated strain had no adverse effect on translocation and the internal organ indices, though significant differences in histology (villi height, crypts height) of ileum were observed. The data acquired suggest that <jats:italic>E<\/jats:italic>. <jats:italic>thailandicus<\/jats:italic> TC1 may be associated with a potential health risk.<\/jats:p>","DOI":"10.1371\/journal.pone.0254081","type":"journal-article","created":{"date-parts":[[2021,7,1]],"date-time":"2021-07-01T18:46:01Z","timestamp":1625165161000},"page":"e0254081","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":14,"title":["Identification and safety assessment of Enterococcus thailandicus TC1 isolated from healthy pigs"],"prefix":"10.1371","volume":"16","author":[{"given":"Xiaoying","family":"Wu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bei","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiue","family":"Jin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7892-8018","authenticated-orcid":true,"given":"Xiliang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2021,7,1]]},"reference":[{"key":"pone.0254081.ref001","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1111\/j.1574-6976.2002.tb00608.x","article-title":"Enterococci from foods","volume":"26","author":"G Giraffa","year":"2002","journal-title":"FEMS Microbiol Rev"},{"key":"pone.0254081.ref002","doi-asserted-by":"crossref","first-page":"3473","DOI":"10.1128\/JCM.00603-08","article-title":"Designation of the provisional new enterococcus species CDC PNS-E2 as Enterococcus sanguinicola sp. nov., isolated from human blood, and identification of a strain previously named Enterococcus CDC PNS-E1 as Enterococcus italicus Fortina, Ricci, Mora, and Manachini 2004","volume":"46","author":"G Carvalho Mda","year":"2008","journal-title":"J Clin Microbiol"},{"key":"pone.0254081.ref003","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.fm.2011.12.002","article-title":"Characterization of functional, safety, and probiotic properties of Enterococcus faecalis UGRA10, a new AS-48-producer strain","volume":"30","author":"R Cebrian","year":"2012","journal-title":"Food Microbiol"},{"key":"pone.0254081.ref004","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1016\/j.ijfoodmicro.2008.01.014","article-title":"A survey on biotechnological potential and safety of the novel Enterococcus species of dairy origin, E. italicus","volume":"123","author":"MG Fortina","year":"2008","journal-title":"Int J Food Microbiol"},{"key":"pone.0254081.ref005","first-page":"1","article-title":"Antibiotic resistance and siderophore production in enterococci","volume":"66","author":"P Lisiecki","year":"2014","journal-title":"Med Dosw Mikrobiol"},{"key":"pone.0254081.ref006","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1538\/expanim.63.297","article-title":"Identification and characterization of vancomycin-resistant Enterococcus species frequently isolated from laboratory mice","volume":"63","author":"H Yamanaka","year":"2014","journal-title":"Exp Anim"},{"key":"pone.0254081.ref007","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/j.fct.2014.05.005","article-title":"Oral toxicity evaluation of kefir-isolated Lactobacillus kefiranofaciens M1 in Sprague-Dawley rats","volume":"70","author":"EE Owaga","year":"2014","journal-title":"Food Chem Toxicol"},{"key":"pone.0254081.ref008","doi-asserted-by":"crossref","first-page":"913","DOI":"10.1128\/JCM.40.3.913-917.2002","article-title":"Enterococcus faecium-related outbreak with molecular evidence of transmission from pigs to humans","volume":"40","author":"HZ Lu","year":"2002","journal-title":"J Clin Microbiol"},{"key":"pone.0254081.ref009","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/j.micpath.2017.01.020","article-title":"Multi-antibiotic resistant and putative virulence gene signatures in Enterococcus species isolated from pig farms environment","volume":"104","author":"A Beshiru","year":"2017","journal-title":"Microb Pathog"},{"key":"pone.0254081.ref010","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.00534-13","article-title":"Emergence of epidemic multidrug-resistant Enterococcus faecium from animal and commensal strains","volume":"4","author":"F Lebreton","year":"2013","journal-title":"mBio"},{"key":"pone.0254081.ref011","doi-asserted-by":"crossref","first-page":"1192","DOI":"10.1128\/JCM.42.3.1192-1198.2004","article-title":"Characterization of three new enterococcal species, Enterococcus sp. nov. 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