{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T03:07:43Z","timestamp":1786676863034,"version":"build-2736575974"},"reference-count":32,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2021,7,20]],"date-time":"2021-07-20T00:00:00Z","timestamp":1626739200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:sec id=\"sec001\">\n<jats:title>Background<\/jats:title>\n<jats:p>Mesenteric ischemia is a severe and potentially lethal event. Assessment of intestine perfusion is eminently depending on the skills, and the experience of the surgeon. Thus, the therapy is biased by the right evaluation. Aim of this study is to determine the applicability, and the usefulness of fluorescent-imaging (FI) with indocyanine green (ICG) in a porcine model of mesenteric ischemia. Second end-point is the verification of a visual and quantitative assessment tool of the intestinal perfusion.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec002\">\n<jats:title>Methods<\/jats:title>\n<jats:p>In 18 pigs (54,2 \u00b12,9kg) an occlusion of a side-branch of the mesenteric artery was performed for 3 (group I, n = 7), 6 (group II, n = 7), and 10 hours (group III, n = 4). After reperfusion a 60 minutes observation period was carried out. 3 regions of interest were defined: ischemic bowel (D1), transitional zone (D2), and non-ischemic bowel (D3). ICG-FI was performed during baseline (T0), occlusion (T1), reperfusion (T2) and after an observation period of 60 minutes (T4).<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec003\">\n<jats:title>Results<\/jats:title>\n<jats:p>All experiments could be finished successfully. ICG-FI was assessed using assessment of background-subtracted peak fluorescence intensity (BSFI), slope of fluorescence intensity (SFI), and a baseline adjusted ratio of both parameters. ICG-FI confirmed loss of perfusion in D1, decreased perfusion in D2, and increased perfusion in D3. After reperfusion ICG-FI increased in group 2 due to a severe tissue damage resulting in a capillary leakage. In group I ICG-FI was equal to baseline values indicating the totally reversible loss of perfusion.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec004\">\n<jats:title>Conclusion<\/jats:title>\n<jats:p>Using ICG-FI to estimate intestine perfusion after different durations of ischemia is viable using a porcine model of mesenteric ischemia. Even small differences in perfusion can be reliably determined by ICG-FI. Thus, ICG-FI is an encouraging method to evaluate intestine perfusion intraoperatively.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pone.0254144","type":"journal-article","created":{"date-parts":[[2021,7,20]],"date-time":"2021-07-20T17:51:59Z","timestamp":1626803519000},"page":"e0254144","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":17,"title":["Intraoperative quality assessment of tissue perfusion with indocyanine green (ICG) in a porcine model of mesenteric ischemia"],"prefix":"10.1371","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3323-122X","authenticated-orcid":true,"given":"Anna","family":"Dupr\u00e9e","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henrik","family":"Rie\u00df","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philipp H.","family":"von Kroge","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jakob R.","family":"Izbicki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eike S.","family":"Debus","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oliver","family":"Mann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans O.","family":"Pinnschmidt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Detlef","family":"Russ","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Detter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabine H.","family":"Wipper","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2021,7,20]]},"reference":[{"issue":"3","key":"pone.0254144.ref001","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1007\/s00595-004-2924-0","article-title":"Acute mesenteric ischemia: the challenge of gastroenterology","volume":"35","author":"H Yasuhara","year":"2005","journal-title":"Surgery today"},{"issue":"3","key":"pone.0254144.ref002","first-page":"489","article-title":"Acute mesenteric ischemia","volume":"114","author":"RJ Stoney","year":"1993","journal-title":"Surgery"},{"issue":"1","key":"pone.0254144.ref003","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1007\/s00423-010-0727-x","article-title":"Intestinal ischemia\/reperfusion: microcirculatory pathology and functional consequences","volume":"396","author":"B Vollmar","year":"2011","journal-title":"Langenbecks Arch Surg"},{"issue":"9","key":"pone.0254144.ref004","doi-asserted-by":"crossref","first-page":"1087","DOI":"10.1007\/s00384-011-1196-6","article-title":"Intestinal ischemia","volume":"26","author":"ES Debus","year":"2011","journal-title":"Int J Colorectal Dis"},{"issue":"2","key":"pone.0254144.ref005","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1007\/s00423-007-0263-5","article-title":"Unchanged high mortality rates from acute occlusive intestinal ischemia: six year review","volume":"393","author":"WT Kassahun","year":"2008","journal-title":"Langenbecks Arch Surg"},{"issue":"1","key":"pone.0254144.ref006","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1007\/s00423-010-0726-y","article-title":"Intestinal ischemia: current treatment concepts. 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