{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:05:07Z","timestamp":1760123107594,"version":"3.37.3"},"reference-count":57,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2021,8,13]],"date-time":"2021-08-13T00:00:00Z","timestamp":1628812800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100010575","name":"Consejo Nacional de Rectores","doi-asserted-by":"publisher","award":["02-2020 and 0652-19"],"award-info":[{"award-number":["02-2020 and 0652-19"]}],"id":[{"id":"10.13039\/501100010575","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005299","name":"Vicerrector\u00eda de Investigaci\u00f3n, Universidad de Costa Rica","doi-asserted-by":"publisher","award":["C0456"],"award-info":[{"award-number":["C0456"]}],"id":[{"id":"10.13039\/501100005299","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005298","name":"Universidad de Costa Rica","doi-asserted-by":"publisher","award":["B8762"],"award-info":[{"award-number":["B8762"]}],"id":[{"id":"10.13039\/501100005298","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Universidad Nacional de Costa Rica","award":["SIA 0047-17"],"award-info":[{"award-number":["SIA 0047-17"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p><jats:italic>Brucella<\/jats:italic> is a facultative extracellular-intracellular pathogen that belongs to the <jats:italic>Alphaproteobacteria<\/jats:italic> class. Precise sensing of environmental changes and a proper response mediated by a gene expression regulatory network are essential for this pathogen to survive. The plant-related <jats:italic>Alphaproteobacteria Sinorhizobium meliloti<\/jats:italic> and <jats:italic>Agrobacterium tumefaciens<\/jats:italic> also alternate from a free to a host-associated life, where a regulatory invasion switch is needed for this transition. This switch is composed of a two-component regulatory system (TCS) and a global inhibitor, ExoR. In <jats:italic>B<\/jats:italic>. <jats:italic>abortus<\/jats:italic>, the BvrR\/BvrS TCS is essential for intracellular survival. However, the presence of a TCS inhibitor, such as ExoR, in <jats:italic>Brucella<\/jats:italic> is still unknown. In this work, we identified a genomic sequence similar to <jats:italic>S<\/jats:italic>. <jats:italic>meliloti exoR<\/jats:italic> in the <jats:italic>B<\/jats:italic>. <jats:italic>abortus<\/jats:italic> 2308W genome, constructed an <jats:italic>exoR<\/jats:italic> mutant strain, and performed its characterization through <jats:italic>ex vivo<\/jats:italic> and <jats:italic>in vivo<\/jats:italic> assays. Our findings indicate that ExoR is related to the BvrR phosphorylation state, and is related to the expression of known BvrR\/BrvS gene targets, such as <jats:italic>virB8<\/jats:italic>, <jats:italic>vjbR<\/jats:italic>, and <jats:italic>omp25<\/jats:italic> when grown in rich medium or starving conditions. Despite this, the <jats:italic>exoR<\/jats:italic> mutant strain showed no significant differences as compared to the wild-type strain, related to resistance to polymyxin B or human non-immune serum, intracellular replication, or infectivity in a mice model. ExoR in <jats:italic>B<\/jats:italic>. <jats:italic>abortus<\/jats:italic> is related to BvrR\/BvrS as observed in other Rhizobiales; however, its function seems different from that observed for its orthologs described in <jats:italic>A<\/jats:italic>. <jats:italic>tumefaciens<\/jats:italic> and <jats:italic>S<\/jats:italic>. <jats:italic>meliloti<\/jats:italic>.<\/jats:p>","DOI":"10.1371\/journal.pone.0254568","type":"journal-article","created":{"date-parts":[[2021,8,13]],"date-time":"2021-08-13T17:27:06Z","timestamp":1628875626000},"page":"e0254568","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":6,"title":["A Sinorhizobium meliloti and Agrobacterium tumefaciens ExoR ortholog is not crucial for Brucella abortus virulence"],"prefix":"10.1371","volume":"16","author":[{"given":"Amanda","family":"Castillo-Zeled\u00f3n","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nazareth","family":"Ruiz-Villalobos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pamela","family":"Altamirano-Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos","family":"Chac\u00f3n-D\u00edaz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4428-3340","authenticated-orcid":true,"given":"El\u00edas","family":"Barquero-Calvo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Esteban","family":"Chaves-Olarte","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1036-920X","authenticated-orcid":true,"given":"Caterina","family":"Guzm\u00e1n-Verri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"340","published-online":{"date-parts":[[2021,8,13]]},"reference":[{"issue":"12","key":"pone.0254568.ref001","doi-asserted-by":"crossref","first-page":"e1929","DOI":"10.1371\/journal.pntd.0001929","article-title":"Clinical Manifestations of Human Brucellosis: A Systematic Review and Meta-Analysis","volume":"6","author":"AS Dean","year":"2012","journal-title":"PLoS Negl Trop Dis"},{"key":"pone.0254568.ref002","first-page":"1152","article-title":"Studies On Brucella abortus Infection In Bulls","volume":"24","author":"G Lambert","year":"1963","journal-title":"Am J Vet Res"},{"issue":"7","key":"pone.0254568.ref003","doi-asserted-by":"crossref","first-page":"2252","DOI":"10.1590\/S0103-84782009005000167","article-title":"The genus Brucella and clinical manifestations of brucellosis","volume":"39","author":"MN Xavier","year":"2009","journal-title":"Cienc Rural"},{"issue":"24","key":"pone.0254568.ref004","first-page":"213","article-title":"Retrospective and prospective perspectives on zoonotic brucellosis","volume":"5","author":"E Moreno","year":"2014","journal-title":"Front. 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