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One contains disulfide bonds exhibiting a shorter (3 days) <jats:italic>in vivo<\/jats:italic> biodegradation time, while the other one has alkyl crosslinks and a longer biodegradation time (more than 7 days). According to the mechanical measurements, the tensile strength of the membranes is comparable to those of soft the connective tissues and visceral tissues. Furthermore, the suture retention test suggests, that the membranes would withstand surgical handling and <jats:italic>in vivo<\/jats:italic> fixation. The <jats:italic>in vivo<\/jats:italic> biocompatibility study demonstrates how membranes undergo <jats:italic>in vivo<\/jats:italic> hydrolysis and by the 3<jats:sup>rd<\/jats:sup> day they become poly(aspartic acid) fibrous membranes, which can be then enzymatically degraded. After one week, the disulfide crosslinked membranes almost completely degrade, while the alkyl-chain crosslinked ones mildly lose their integrity as the surrounding tissue invades them. Histopathology revealed mild acute inflammation, which diminished to a minimal level after seven days.<\/jats:p>","DOI":"10.1371\/journal.pone.0254843","type":"journal-article","created":{"date-parts":[[2021,8,13]],"date-time":"2021-08-13T17:42:34Z","timestamp":1628876554000},"page":"e0254843","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":15,"title":["Poly(amino acid) based fibrous membranes with tuneable in vivo biodegradation"],"prefix":"10.1371","volume":"16","author":[{"given":"Kristof","family":"Molnar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Constantinos","family":"Voniatis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniella","family":"Feher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gyorgyi","family":"Szabo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rita","family":"Varga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lilla","family":"Reiniger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Juriga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zoltan","family":"Kiss","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eniko","family":"Krisch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gyorgy","family":"Weber","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Ferencz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabor","family":"Varga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miklos","family":"Zrinyi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krisztina S.","family":"Nagy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2720-783X","authenticated-orcid":true,"given":"Angela","family":"Jedlovszky-Hajdu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2021,8,13]]},"reference":[{"key":"pone.0254843.ref001","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1016\/j.eurpolymj.2014.11.024","article-title":"Biomedical applications of hydrogels: A review of patents and commercial products","volume":"65","author":"E Cal\u00f3","year":"2015","journal-title":"Eur Polym J"},{"key":"pone.0254843.ref002","doi-asserted-by":"crossref","first-page":"20356","DOI":"10.1021\/acsami.8b06262","article-title":"Hydrogel Effects Rapid Biofilm Debridement with ex situ Contact-Kill to Eliminate Multidrug Resistant Bacteria in vivo","volume":"10","author":"CK Yeo","year":"2018","journal-title":"ACS Appl Mater Interfaces"},{"key":"pone.0254843.ref003","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1016\/j.jmbbm.2018.08.013","article-title":"The relationship between thiol-acrylate photopolymerization kinetics and hydrogel mechanics: An improved model incorporating photobleaching and thiol-Michael addition","volume":"88","author":"H Zhu","year":"2018","journal-title":"J Mech Behav Biomed Mater"},{"key":"pone.0254843.ref004","doi-asserted-by":"crossref","first-page":"12374","DOI":"10.1021\/acsami.7b17751","article-title":"Engineering the Cell Microenvironment Using Novel Photoresponsive Hydrogels","volume":"10","author":"Y Dong","year":"2018","journal-title":"ACS Appl Mater Interfaces"},{"key":"pone.0254843.ref005","article-title":"A dual-enzymatically cross-linked injectable gelatin hydrogel loaded with BMSC improves neurological function recovery of traumatic brain injury in rats","author":"M Yao","year":"2019","journal-title":"Biomater Sci"},{"key":"pone.0254843.ref006","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/B978-0-12-813665-2.00011-9","article-title":"Hydrogels for biomedical applications","volume":"64","author":"LR Feksa","year":"2018","journal-title":"Nanostructures Eng Cells, Tissues Organs From Des to Appl."},{"key":"pone.0254843.ref007","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1002\/adma.200800534","article-title":"Hydrogels for soft machines","volume":"21","author":"P. 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