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Ltd."}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Myxomatous mitral valve disease (MMVD) is characterized by thickening of the valve leaflets and omega-3 (\u03c9-3) supplementation has been associated with modulation of blood pressure (BP) and heart rate, improvement of doppler echocardiographic indices, antiarrhythmic, anti-inflammatory and anti-dislipidemic effects in dogs and humans, although prospective studies of it single use are still absent in the veterinary literature. The objective of this study was to evaluate the influence of \u03c9-3 supplementation in dogs with MMVD. Twenty-nine dogs were followed quarterly for 12 months by clinical evaluation, arterial blood pressure, electrocardiography, doppler echocardiography, thoracic radiography and laboratory tests including inflammatory mediators and cardiac biomarker blood concentrations. The dogs were classified in stages B2 and C, according to the classification proposed by ACVIM 2019. They were randomly assigned to either \u03c9-3 group (\u03c9-3G) or control group (CG). The ingestion of \u03c9-3 reduced the chance of developing arrhythmias by 2.96 times (p = 0.003). The vertebral heart size (VHS) measurements were higher in the control group (p = 0.033). In conclusion, at the dosages used in this study, \u03c9-3 dietary supplementation reduces the volumetric overload, has antiarrhythmic effect and keeps dogs with B2 and C stages of MMVD in milder stages of the disease.<\/jats:p>","DOI":"10.1371\/journal.pone.0254887","type":"journal-article","created":{"date-parts":[[2021,7,15]],"date-time":"2021-07-15T17:39:15Z","timestamp":1626370755000},"page":"e0254887","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":10,"title":["Protective effects of omega-3 fatty acids in dogs with myxomatous mitral valve disease stages B2 and C"],"prefix":"10.1371","volume":"16","author":[{"given":"Priscilla Regina","family":"Nasciutti","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aline Tavares","family":"Moraes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thaiz Krawczyk","family":"Santos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karine Kelly","family":"Gon\u00e7alves Queiroz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Paula Ara\u00fajo","family":"Costa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andressa Rodrigues","family":"Amaral","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigo","family":"Fernando Gomes Olivindo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristiana Ferreira Fonseca","family":"Pontieri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliana Toloi","family":"Jeremias","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thiago Henrique Annibale","family":"Vendramini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3240-0343","authenticated-orcid":true,"given":"Marcio Antonio","family":"Brunetto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ros\u00e2ngela de Oliveira Alves","family":"Carvalho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2021,7,15]]},"reference":[{"key":"pone.0254887.ref001","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.jvc.2012.01.011","article-title":"Historical review, epidemiology and natural history of degenerative mitral valve disease","volume":"14","author":"M Borgarelli","year":"2012","journal-title":"J Vet Cardiol"},{"key":"pone.0254887.ref002","doi-asserted-by":"crossref","first-page":"1127","DOI":"10.1111\/jvim.15488","article-title":"ACVIM consensus guidelines for the diagnosis and treatment of myxomatous mitral valve disease in dogs","volume":"33","author":"BW Keene","year":"2019","journal-title":"J Vet Intern Med"},{"key":"pone.0254887.ref003","doi-asserted-by":"crossref","first-page":"1142","DOI":"10.1111\/j.1939-1676.2009.0392.x","article-title":"Guidelines for the Diagnosis and Treatment of Canine Chronic Valvular Heart Disease","volume":"23","author":"C Atkins","year":"2009","journal-title":"J Vet Intern Med"},{"key":"pone.0254887.ref004","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1111\/j.1939-1676.2011.00838.x","article-title":"Cachexia and sarcopenia: Emerging syndromes of importance in dogs and cats","volume":"26","author":"LM Freeman","year":"2012","journal-title":"J Vet Intern Med"},{"key":"pone.0254887.ref005","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/j.jvc.2012.02.001","article-title":"Pathology of myxomatous mitral valve disease in the dog","volume":"14","author":"PR Fox","year":"2012","journal-title":"J Vet Cardiol"},{"key":"pone.0254887.ref006","first-page":"482","article-title":"Review on nutritional management of cardiac disorders in canines","author":"S Devi","year":"2009","journal-title":"Veterinary World"},{"key":"pone.0254887.ref007","doi-asserted-by":"crossref","DOI":"10.1111\/j.1939-1676.2008.0071.x","article-title":"Association of Body Weight and Body Condition with Survival in Dogs with Heart Failure","volume":"22","author":"JL Slupe","year":"2008","journal-title":"J Vet Intern Med"},{"key":"pone.0254887.ref008","first-page":"743","article-title":"Recent development in research and management of cancer anorexia-cachexia syndrome","author":"A. 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