{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T10:07:17Z","timestamp":1791540437967,"version":"4.3.4"},"reference-count":44,"publisher":"Public Library of Science (PLoS)","issue":"7","license":[{"start":{"date-parts":[[2021,7,26]],"date-time":"2021-07-26T00:00:00Z","timestamp":1627257600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Start-up grant from CTAHR University of Hawaii at Manoa, and USDA Multistate","award":["2052R"],"award-info":[{"award-number":["2052R"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>\n                    Heat stress accounts for substantial economic loss in the poultry industry by altering the health and performance of chickens. Alpha-lipoic acid (ALA) is a water and fat-soluble antioxidant which is readily absorbed from the intestine resulting in maximum bioavailability. Moreover, ALA acts as a coenzyme in glucose metabolism and helps generate other antioxidants. Considering these benefits, we hypothesized that dietary supplementation of ALA would help mitigate heat stress in poultry. A total of 72 Day-old broiler chicks were randomly assigned into three treatment groups: no heat stress (NHS), heat stress with basal diet (HS), and heat stress with alpha-lipoic acid (HS+ALA); each treatment group had 6 replicate pens with 4 birds in each pen (n = 24\/group). The allocated birds were raised under standard husbandry practices for 3 weeks. After 21 d, birds in the HS and HS+ALA groups were exposed to heat stress (33\u00b0C for 8 hours during the day) for 3 weeks, while the NHS group was reared under normal conditions (22\u201324\u00b0C). The HS+ALA group received a basal finisher diet fortified with ALA (500 mg\/kg) during the treatment period (22 to 42 d), while other birds were provided with the basal finisher diet. Weekly body weight and feed intake were recorded. The cecum digesta for volatile fatty acids (VFAs) analysis and 16S rRNA sequencing for the gut microbiota analysis; and the ileum tissue samples for histological and gene expression analyses were collected on d 42. Exposure to heat stress decreased (P&lt;0.05) average daily gain (ADG) and final body weight (FBW) in the HS group compared to the NHS group, the supplementation of ALA improved (P&lt;0.05) ADG and FBW in heat-stressed birds. Furthermore, birds in the HS+ALA group had increased (P&lt;0.05) expression of\n                    <jats:italic>HSP90<\/jats:italic>\n                    ,\n                    <jats:italic>PRDX1<\/jats:italic>\n                    ,\n                    <jats:italic>GPX3<\/jats:italic>\n                    ,\n                    <jats:italic>SOD2<\/jats:italic>\n                    ,\n                    <jats:italic>OCLN<\/jats:italic>\n                    , and\n                    <jats:italic>MUC2<\/jats:italic>\n                    genes and higher (P&lt;0.05) concentrations of major VFAs (acetate, propionate, and butyrate). The dietary ALA supplementation also improved the villus height and villus height to crypt depth ratio in the HS+ALA group. The microbial diversity analysis revealed significant abundance (P&lt;0.05) of beneficial bacteria\n                    <jats:italic>Lactobacillus<\/jats:italic>\n                    and\n                    <jats:italic>Peptostreptococcaceae<\/jats:italic>\n                    in the cecum of the ALA group. These results indicate that dietary ALA supplementation effectively mitigates the negative effects of heat stress in broilers by improving the expression of heat-shock, tight-junction, antioxidants, and immune-related genes in the intestine, improving villus structures, increasing concentration of major VFAs, and enriching the beneficial microbiota.\n                  <\/jats:p>","DOI":"10.1371\/journal.pone.0254936","type":"journal-article","created":{"date-parts":[[2021,7,26]],"date-time":"2021-07-26T13:33:13Z","timestamp":1627306393000},"page":"e0254936","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":21,"title":["Dietary supplementation of alpha-lipoic acid mitigates the negative effects of heat stress in broilers"],"prefix":"10.1371","volume":"16","author":[{"given":"Sanjeev","family":"Wasti","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nirvay","family":"Sah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chin N.","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2891-8353","authenticated-orcid":true,"given":"Rajesh","family":"Jha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8786-3700","authenticated-orcid":true,"given":"Birendra","family":"Mishra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2021,7,26]]},"reference":[{"key":"pone.0254936.ref001","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/0020-711X(94)90190-2","article-title":"Rapidly growing broiler (meat-type) chickens. 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