{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T05:36:50Z","timestamp":1782193010003,"version":"3.54.5"},"reference-count":58,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2021,8,12]],"date-time":"2021-08-12T00:00:00Z","timestamp":1628726400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"faculty of graduate studies, mahidol university","award":["research assistance"],"award-info":[{"award-number":["research assistance"]}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Mitral valve disease (MVD) is an important and most frequently acquired heart disease found in dogs. MVD is classified into different stages according to its severity. There is a challenge in differentiation between asymptomatic and symptomatic stages of the MVD. Moreover, pulmonary hypertension (PH) is a common complication in dogs affected by MVD. In clinical practice, there are also some limitations to identify PH. Matrix-assisted laser desorption\/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a technique that can characterize specific patterns of peptide mass called peptide barcodes from various samples. Besides, in combination with liquid chromatography-tandem mass spectrometry (LC-MS\/MS), potential peptide sequences associated with specific conditions could be identified. The present study aimed to use MALDI-TOF coupled with LC-MS\/MS to characterize specific peptide barcodes and potential peptide candidates in serum samples from healthy dogs, dogs with MVD stage B (MVD B, asymptomatic stage), MVD stage C (MVD C, symptomatic stage), MVD stage B with PH (MVD B PH), and MVD stage C with PH (MVD C PH). Discrete clusters of the 5 sample groups were identified by 3D plot analysis. Peptide barcodes also revealed differences in peptide patterns among the 5 groups. Six amino acid sequences of peptide candidates at 1,225.60, 1,363.85, 1,688.71, 1789.52, 2020.21, and 2156.42 Da were identified as part of the proteins CLCN1, CLUL1, EDNRA, PTEN, SLC39A7, and CLN6, respectively. The network interactions between these discovered proteins and common cardiovascular drugs were also investigated. These results demonstrate that MALDI-TOF MS has promise as an optional technique for diagnosing dogs affected by asymptomatic and symptomatic stages of MVD with and without PH. Further studies are required to identify peptide barcodes in dogs with other diseases to create peptide barcode databases in veterinary medicine before using this method as a novel diagnostic tool in the future.<\/jats:p>","DOI":"10.1371\/journal.pone.0255611","type":"journal-article","created":{"date-parts":[[2021,8,12]],"date-time":"2021-08-12T19:02:22Z","timestamp":1628794942000},"page":"e0255611","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":9,"title":["Peptide barcodes in dogs affected by mitral valve disease with and without pulmonary hypertension using MALDI-TOF MS and 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Med"},{"issue":"1","key":"pone.0255611.ref040","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1111\/j.1748-5827.2007.00510.x","article-title":"The diagnostic accuracy of different natriuretic peptides in the investigation of canine cardiac disease","volume":"49","author":"A Boswood","year":"2008","journal-title":"J Small Anim Pract"},{"issue":"2","key":"pone.0255611.ref041","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/j.tvjl.2007.12.026","article-title":"Predictive value of natriuretic peptides in dogs with mitral valve disease","volume":"180","author":"I Tarnow","year":"2009","journal-title":"Vet J"},{"issue":"3","key":"pone.0255611.ref042","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1016\/j.jvc.2012.05.005","article-title":"Lowered N-terminal pro-B-type natriuretic peptide levels in response to treatment predict survival in dogs with symptomatic mitral valve disease","volume":"14","author":"J Wolf","year":"2012","journal-title":"J Vet Cardiol"},{"issue":"2","key":"pone.0255611.ref043","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1111\/j.1748-5827.2009.00889.x","article-title":"N-terminal pro B-type natriuretic peptide and left ventricular diameter independently predict mortality in dogs with mitral valve disease","volume":"51","author":"W Moonarmart","year":"2010","journal-title":"J Small Anim Pract"},{"issue":"9","key":"pone.0255611.ref044","doi-asserted-by":"crossref","first-page":"1072","DOI":"10.14202\/vetworld.2017.1072-1082","article-title":"N-terminal-pro brain natriuretic peptides in dogs and cats: A technical and clinical review","volume":"10","author":"GV de Lima","year":"2017","journal-title":"Vet World"},{"issue":"1","key":"pone.0255611.ref045","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1111\/jvim.13801","article-title":"Cardiac Troponins in Dogs and Cats","volume":"30","author":"R Langhorn","year":"2016","journal-title":"J Vet Intern Med"},{"issue":"2","key":"pone.0255611.ref046","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/S0009-8981(99)00079-0","article-title":"Clinical insights on the use of highly sensitive cardiac troponin assays","volume":"284","author":"ED Missov","year":"1999","journal-title":"Clin Chim Acta"},{"issue":"33","key":"pone.0255611.ref047","doi-asserted-by":"crossref","first-page":"20545","DOI":"10.1074\/jbc.272.33.20545","article-title":"Stretch-induced hypertrophic growth of cardiocytes and processing of brain-type natriuretic peptide are controlled by proprotein-processing endoprotease furin","volume":"272","author":"Y Sawada","year":"1997","journal-title":"J Biol Chem"},{"issue":"12","key":"pone.0255611.ref048","doi-asserted-by":"crossref","first-page":"1078","DOI":"10.1212\/WNL.0b013e31828868e7","article-title":"Novel brain expression of ClC-1 chloride channels and enrichment of CLCN1 variants in epilepsy","volume":"80","author":"TT Chen","year":"2013","journal-title":"Neurology"},{"issue":"10","key":"pone.0255611.ref049","doi-asserted-by":"crossref","first-page":"4542","DOI":"10.1167\/iovs.02-1202","article-title":"Comparative analysis and expression of CLUL1, a cone photoreceptor-specific gene","volume":"44","author":"Q Zhang","year":"2003","journal-title":"Invest Ophthalmol Vis Sci"},{"issue":"6303","key":"pone.0255611.ref050","doi-asserted-by":"crossref","first-page":"730","DOI":"10.1038\/348730a0","article-title":"Cloning and expression of a cDNA encoding an endothelin receptor","volume":"348","author":"H Arai","year":"1990","journal-title":"Nature"},{"issue":"6303","key":"pone.0255611.ref051","doi-asserted-by":"crossref","first-page":"732","DOI":"10.1038\/348732a0","article-title":"Cloning of a cDNA encoding a non-isopeptide-selective subtype of the endothelin receptor","volume":"348","author":"T Sakurai","year":"1990","journal-title":"Nature"},{"issue":"2","key":"pone.0255611.ref052","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1007\/s00018-010-0518-0","article-title":"Endothelin","volume":"68","author":"Y Kawanabe","year":"2011","journal-title":"Cell Mol Life Sci"},{"issue":"22","key":"pone.0255611.ref053","doi-asserted-by":"crossref","first-page":"1922","DOI":"10.1093\/jnci\/91.22.1922","article-title":"Mutational spectra of PTEN\/MMAC1 gene: a tumor suppressor with lipid phosphatase activity","volume":"91","author":"IU Ali","year":"1999","journal-title":"J Natl Cancer Inst"},{"issue":"3","key":"pone.0255611.ref054","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1354\/vp.39-3-372","article-title":"Tumor suppressor PTEN is mutated in canine osteosarcoma cell lines and tumors","volume":"39","author":"RA Levine","year":"2002","journal-title":"Vet 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