{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T01:25:13Z","timestamp":1787966713253,"version":"build-2784847793"},"reference-count":38,"publisher":"Public Library of Science (PLoS)","issue":"9","license":[{"start":{"date-parts":[[2021,9,2]],"date-time":"2021-09-02T00:00:00Z","timestamp":1630540800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100014125","name":"Fujian Provincial Health Commission","doi-asserted-by":"crossref","award":["2016-1-5"],"award-info":[{"award-number":["2016-1-5"]}],"id":[{"id":"10.13039\/501100014125","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100013795","name":"Fujian Medical University","doi-asserted-by":"crossref","award":["2016QH108"],"award-info":[{"award-number":["2016QH108"]}],"id":[{"id":"10.13039\/501100013795","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100017543","name":"Fujian Provincial Hospital","doi-asserted-by":"crossref","award":["2014YNZD04"],"award-info":[{"award-number":["2014YNZD04"]}],"id":[{"id":"10.13039\/501100017543","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:sec id=\"sec001\">\n<jats:title>Aim<\/jats:title>\n<jats:p>To determine the minimum inhibitory concentrations (MICs) of commonly used antibiotics against <jats:italic>Helicobacter Pylori<\/jats:italic> (<jats:italic>H<\/jats:italic>. <jats:italic>pylori<\/jats:italic>) in South China and compare their resistance rates by using EUCAST breakpoints and other breakpoints.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec002\">\n<jats:title>Methods<\/jats:title>\n<jats:p>Patients who had not previously received <jats:italic>H<\/jats:italic>. <jats:italic>pylori<\/jats:italic> treatment in clinical centers in South China were enrolled in this study from 2017 to 2020. Gastric biopsies were obtained for <jats:italic>H<\/jats:italic>. <jats:italic>pylori<\/jats:italic> culture. The MICs of amoxicillin (AMX), clarithromycin (CLA), metronidazole (MTZ), levofloxacin (LEV), tetracycline (TET) and furazolidone (FZD) were tested by broth microdilution method and assessed by two different breakpoints. ATCC43504 standard strain served as a control.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec003\">\n<jats:title>Results<\/jats:title>\n<jats:p>A total of 208 <jats:italic>H<\/jats:italic>. <jats:italic>pylori<\/jats:italic> strains were isolated from patients\u2019 biopsy samples. The MICs of AMX, CLA, MTZ, LEV, TET and FZD for <jats:italic>H<\/jats:italic>. <jats:italic>pylori<\/jats:italic> were 0.0156-256mg\/L (MIC<jats:sub>50<\/jats:sub> 0.125mg\/L, MIC<jats:sub>90<\/jats:sub> 4mg\/L), 0.0156- &gt;256 mg\/L (MIC<jats:sub>50<\/jats:sub> 0.0312mg\/L, MIC<jats:sub>90<\/jats:sub> 64mg\/L), 0.0156- &gt;256mg\/L (MIC<jats:sub>50<\/jats:sub> 8mg\/L, MIC<jats:sub>90<\/jats:sub> 256mg\/L), 0.0156-256mg\/L (MIC<jats:sub>50<\/jats:sub> 0.25mg\/L, MIC<jats:sub>90<\/jats:sub> 16mg\/L), 0.0156-256mg\/L (MIC<jats:sub>50<\/jats:sub> 0.0625mg\/L, MIC<jats:sub>90<\/jats:sub> 4mg\/L), and 0.0156- &gt;256mg\/L (MIC<jats:sub>50<\/jats:sub> 0.0312mg\/L, MIC<jats:sub>90<\/jats:sub> 2mg\/L), respectively. The MICs of AMX, CLA, MTZ, LEV, TET and FZD for ATCC43504 strain were 0.25mg\/L, 0.0625mg\/L, 64mg\/L, 0.5mg\/L, 1mg\/L and 0.25mg\/L, respectively. The resistance rate of FZD was 11.05%. The overall resistance rates according to EUCAST breakpoints and other breakpoints were 57.21% and 14.90% for AMX (<jats:italic>p<\/jats:italic>&lt;0.001), 38.94% and 38.94% for CLA (<jats:italic>p<\/jats:italic> = 1), 39.42% and 50.96% for MTZ (<jats:italic>p<\/jats:italic>&lt;0.001), 12.98% and 10.58% for TET (<jats:italic>p<\/jats:italic> = 0.025), 35.10% and 35.10% for LEV (<jats:italic>p<\/jats:italic> = 1), respectively.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"sec004\">\n<jats:title>Conclusions<\/jats:title>\n<jats:p>Our results demonstrate that AMX, FZD, and TET, but not MTZ, CLR or LEV, showed good anti-<jats:italic>H<\/jats:italic>. <jats:italic>pylori<\/jats:italic> activity <jats:italic>in vitro<\/jats:italic> in South China. When different breakpoints were used, similar results were found with CLA, and LEV, but not with AMX, MTZ, or TET.<\/jats:p>\n<\/jats:sec>","DOI":"10.1371\/journal.pone.0256225","type":"journal-article","created":{"date-parts":[[2021,9,2]],"date-time":"2021-09-02T18:13:12Z","timestamp":1630606392000},"page":"e0256225","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":22,"title":["Minimum inhibitory concentrations of commonly used antibiotics against Helicobacter Pylori: A multicenter study in South China"],"prefix":"10.1371","volume":"16","author":[{"given":"Xueping","family":"Huang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhihui","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baihe","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gaohui","family":"Nong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yushan","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuping","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinhua","family":"Ji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Biao","family":"Suo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiuzhao","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9448-985X","authenticated-orcid":true,"given":"Jinqi","family":"Wei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"340","published-online":{"date-parts":[[2021,9,2]]},"reference":[{"issue":"1","key":"pone.0256225.ref001","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1136\/gutjnl-2016-312288","article-title":"Management of Helicobacter pylori infection-the Maastricht V\/Florence Consensus Report","volume":"66","author":"P Malfertheiner","year":"2017","journal-title":"Gut"},{"issue":"3","key":"pone.0256225.ref002","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1111\/j.1440-1746.2009.06188.x","article-title":"Epidemiology of Helicobacter pylori infection and gastric cancer in Asia","volume":"25","author":"KM Fock","year":"2010","journal-title":"Journal of gastroenterology and hepatology"},{"issue":"4","key":"pone.0256225.ref003","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1016\/j.gastrohep.2017.12.009","article-title":"Update on the management of Helicobacter pylori infection. 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