{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T14:17:59Z","timestamp":1760710679717,"version":"3.40.5"},"reference-count":14,"publisher":"Public Library of Science (PLoS)","issue":"8","license":[{"start":{"date-parts":[[2021,8,18]],"date-time":"2021-08-18T00:00:00Z","timestamp":1629244800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.plosone.org"],"crossmark-restriction":false},"short-container-title":["PLoS ONE"],"abstract":"<jats:p>Efficient and effective viral detection methodologies are a critical piece in the global response to COVID-19, with PCR-based nasopharyngeal and oropharyngeal swab testing serving as the current gold standard. With over 100 million confirmed cases globally, the supply chains supporting these PCR testing efforts are under a tremendous amount of stress, driving the need for innovative and accurate diagnostic solutions. Herein, the utility of a direct-to-PCR method of SARS-CoV-2 detection grounded in mechanical homogenization is examined for reducing resources needed for testing while maintaining a comparable sensitivity to the current gold standard workflow of nasopharyngeal and oropharyngeal swab testing. In a head-to-head comparison of 30 patient samples, this initial clinical validation study of the proposed homogenization-based workflow demonstrated significant agreeability with the current extraction-based method utilized while cutting the total resources needed in half.<\/jats:p>","DOI":"10.1371\/journal.pone.0256316","type":"journal-article","created":{"date-parts":[[2021,8,18]],"date-time":"2021-08-18T17:38:26Z","timestamp":1629308306000},"page":"e0256316","update-policy":"https:\/\/doi.org\/10.1371\/journal.pone.corrections_policy","source":"Crossref","is-referenced-by-count":8,"title":["Validation of a direct-to-PCR COVID-19 detection protocol utilizing mechanical homogenization: A model for reducing resources needed for accurate testing"],"prefix":"10.1371","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5719-0947","authenticated-orcid":true,"given":"Zachary 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colorimetric LAMP","volume":"12","author":"L Bokelmann","year":"2021","journal-title":"Nat Commun"},{"key":"pone.0256316.ref009","doi-asserted-by":"crossref","first-page":"627679","DOI":"10.3389\/fmed.2021.627679","article-title":"SARS-CoV-2 Direct Detection Without RNA Isolation With Loop-Mediated Isothermal Amplification (LAMP) and CRISPR-Cas12","volume":"8","author":"A Garcia-Venzor","year":"2021","journal-title":"Front Med (Lausanne)"},{"issue":"1","key":"pone.0256316.ref010","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1186\/s12985-020-01405-y","article-title":"A novel two-step, direct-to-PCR method for virus detection off swabs using human coronavirus 229E","volume":"17","author":"ZP Morehouse","year":"2020","journal-title":"Virol J"},{"key":"pone.0256316.ref011","unstructured":"Interim Guidelines for Collecting, Handling, and Testing Clinical Specimens from Persons for Coronavirus Disease 2019 (COVID-19) 2020. [Accessed 01\/25\/2021]. https:\/\/www.cdc.gov\/coronavirus\/2019-nCoV\/lab\/guidelines-clinical-specimens.html. 2021."},{"issue":"3","key":"pone.0256316.ref012","first-page":"184","article-title":"Laboratory Tests for COVID-19: A Review of Peer-Reviewed Publications and Implications for Clinical Use","volume":"117","author":"D Shyu","year":"2020","journal-title":"Mo Med"},{"issue":"5","key":"pone.0256316.ref013","doi-asserted-by":"crossref","first-page":"e00297","DOI":"10.1128\/JCM.00297-20","article-title":"Negative Nasopharyngeal and Oropharyngeal Swabs Do Not Rule Out COVID-19","volume":"58","author":"P Winichakoon","year":"2020","journal-title":"J Clin Microbiol"},{"issue":"18","key":"pone.0256316.ref014","first-page":"1843","article-title":"Detection of SARS-CoV-2 in Different Types of Clinical Specimens","volume":"323","author":"W Wang","year":"2020","journal-title":"JAMA"}],"container-title":["PLOS ONE"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0256316","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,8,18]],"date-time":"2021-08-18T17:39:15Z","timestamp":1629308355000},"score":1,"resource":{"primary":{"URL":"https:\/\/dx.plos.org\/10.1371\/journal.pone.0256316"}},"subtitle":[],"editor":[{"given":"Etsuro","family":"Ito","sequence":"first","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2021,8,18]]},"references-count":14,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2021,8,18]]}},"URL":"https:\/\/doi.org\/10.1371\/journal.pone.0256316","relation":{},"ISSN":["1932-6203"],"issn-type":[{"type":"electronic","value":"1932-6203"}],"subject":[],"published":{"date-parts":[[2021,8,18]]}}}